Specialized master mixes for molecular diagnostic assays.
Novel RT-qPCR Reagents Specialized master mixes for molecular diagnostic assays
Meridian’s portfolio of RT-qPCR master mixes have been designed to simplify the development of molecular assays and enable new features such as extraction-free amplification. Each master mix contains a hot-start polymerase, dNTPs, buffer and other components optimized for each particular application (e.g. lyophilization). Only primers and probes are required to complete the assay formula. By using a master mix in which the upstream formulation chemistry has already been refined for superior performance under fast or challenging cycling conditions, assay development can be concentrated to other elements of the assay design. Important features for successful next generation molecular assays include earlier detection, simplified workflow format and shorter time to results.
RT-qPCR Master Mixes for a range of applications
Extraction-free assays
Automated platforms
Ambient temperature assays
Multiplex detection
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Product Selection Chart
Inhibitor-Tolerant RT-qPCR Mix
Fast 1-Step RT-qPCR Mix
Low LOD 1-Step RT-qPCR
Lyo-Ready ™ 1-Step RT-qPCR Mix Pre-formulated with lyo-excipients for lyophilization into beads or cakes
Air-Dryable ™ 1-Step RT-qPCR Mix Ready-to-use mix compatible with a range of air-drying protocols to produce an ambient temperature stable assay
Optimized for extraction-free assays from crude clinical specimens (sputum, saliva and stool)
Ideal for multiplex assays on fast, automated high- throughput systems
Designed for multiplex detection of viruses for applications such as blood bank testing
Features
MDX016
MDX032
MDX025
MDX024
MDX095
Cat#
4x
2x
2x
2x
4x
Concentration
3 tubes (Polymerase, Reverse Transcriptase, RNase Inhibitor)
3 tubes (Polymerase, Reverse Transcriptase, RNase Inhibitor)
Presentation
Single-tube mix
Single tube mix
Single tube mix
Master Mix
Hot-Start
Antibody
Antibody
Antibody
Antibody
Antibody
Ambient- Temperature Assays
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-
-
Multiplex Reactions
RNA/DNA Extraction-free protocols
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-
-
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Contains dUTP
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RT-qPCR Extraction Control • Monitors assay inhibition • Suitable for use with inhibitor-rich samples MDX028 (Red, Quasar 670) MDX029 (Orange, Cal Fluor Orange)
VLP RNA Extraction Control
• Closely mimics the test sample • Undergoes the same processing from lysis and extraction to detection • Compatible with lyophilization for creating freeze-dried mixes
MDX068 (Red, Cy5) MDX069 (Orange, HEX) MDX071 (Custom)
Recommended
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com
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