Novel RT-qPCR Reagents

Novel RT-qPCR Reagents Specialized master mixes for molecular diagnostic assays

Meridian’s portfolio of RT-qPCR master mixes have been designed to simplify the development of molecular assays and enable new features such as extraction-free amplification. Each master mix contains a hot-start polymerase, dNTPs, buffer and other components optimized for each particular application (e.g. lyophilization). Only primers and probes are required to complete the assay formula. By using a master mix in which the upstream formulation chemistry has already been refined for superior performance under fast or challenging cycling conditions, assay development can be concentrated to other elements of the assay design. Important features for successful next generation molecular assays include earlier detection, simplified workflow format and shorter time to results.

RT-qPCR Master Mixes for a range of applications

Extraction-free assays

Automated platforms

Ambient temperature assays

Multiplex detection

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Product Selection Chart

Inhibitor-Tolerant RT-qPCR Mix

Fast 1-Step RT-qPCR Mix

Low LOD 1-Step RT-qPCR

Lyo-Ready ™ 1-Step RT-qPCR Mix Pre-formulated with lyo-excipients for lyophilization into beads or cakes

Air-Dryable ™ 1-Step RT-qPCR Mix Ready-to-use mix compatible with a range of air-drying protocols to produce an ambient temperature stable assay

Optimized for extraction-free assays from crude clinical specimens (sputum, saliva and stool)

Ideal for multiplex assays on fast, automated high- throughput systems

Designed for multiplex detection of viruses for applications such as blood bank testing

Features

MDX016

MDX032

MDX025

MDX024

MDX095

Cat#

4x

2x

2x

2x

4x

Concentration

3 tubes (Polymerase, Reverse Transcriptase, RNase Inhibitor)

3 tubes (Polymerase, Reverse Transcriptase, RNase Inhibitor)

Presentation

Single-tube mix

Single tube mix

Single tube mix

Master Mix

Hot-Start

Antibody

Antibody

Antibody

Antibody

Antibody

Ambient- Temperature Assays

-

-

-

Multiplex Reactions

RNA/DNA Extraction-free protocols

-

-

-

-

Contains dUTP

-

-

-

-

RT-qPCR Extraction Control • Monitors assay inhibition • Suitable for use with inhibitor-rich samples MDX028 (Red, Quasar 670) MDX029 (Orange, Cal Fluor Orange)

VLP RNA Extraction Control

• Closely mimics the test sample • Undergoes the same processing from lysis and extraction to detection • Compatible with lyophilization for creating freeze-dried mixes

MDX068 (Red, Cy5) MDX069 (Orange, HEX) MDX071 (Custom)

Recommended

Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299

Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com

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