SARS-CoV-2 Antibody Pairs for Rapid COVID-19 Antigen Assays
SARS-CoV-2 Antibody Pairs For Rapid COVID-19 Antigen Assays
In May 2020, the FDA announced the approval of the first antigen test to help in the rapid detection of the virus that causes COVID-19. The performance of a rapid antigen test is limited by the sensitivity of the antibodies used. Meridian’s antibodies are highly sensitive for the detection of SARS-CoV-2 and they are ideal for developing reliable and sensitive rapid antigen assays for the detection of active COVID-19 infections. Meridian’s novel monoclonal antibody pair to trimeric SARS-CoV-2 S1 protein is capable of recognizing the spike 1 (S1) protein in saliva samples regardless of its confirmation state (e.g. “up” or “down”). The antibodies bind to a linear epitope on the S1 protein that is distinct from the RBD protein which has epitopes that are conformation-dependent. Recent studies have shown that linear epitopes on the S1 protein are able elicit neutralizing antibodies in COVID-19 patients and have potent antigenicity, making them prime targets for diagnostic, therapeutic and vaccine targets.
Li, Y., Lai, D., Zhang, H. et al. Linear epitopes of SARS-CoV-2 spike protein elicit neutralizing antibodies in COVID-19 patients. Cell Mol Immunol (2020).
NUCLEOCAPSID ANTIBODIES
For use in lateral flow antigen detection COVID-19 assays Highly sensitive antibody pair currently used in commercial diagnostic kits worldwide (LOD: 2.87 x 10 3 TCID 50 / mL, heat-inactivated culture fluid) Manufactured in multigram quantities weekly No cross-reaction with other respiratory pathogens, e.g., MERS-Coronavirus, Human Coronavirus (NL63, 229E and OC43), Influenza A, Influenza B, Respiratory Syncytial Virus (RSV) (A and B), Streptococcus A, Mycoplasma, Human Adenovirus (Types 1, 3, 5, 7, 8, 11, 18, and 23), Human Parainfluenza Virus (Types 1, 2, 3 and 4), Human Rhinovirus (Types 1, 14 and 42) and Human Metapneumovirus
Cat# 9548
MAb to SARS-CoV-2 NP
*pairs with both detection antibodies 9547 and 9549
Cat# 9547
MAb to SARS-CoV-2 NP
Cat# 9549
MAb to SARS-CoV-2 NP
www.MeridianLifeScience.com
TRIMERIC SPIKE ANTIBODIES
For use in lateral flow antigen detection COVID-19 assays Designed to work on saliva samples (no lysis required) Novel antibody pair to the trimeric form of the Spike 1 surface glycoprotein, recognizes both the G614 and D614G strains Using a mixture of detection antibodies Cat# 9565 and #9551 in a 1:1 ratio creates a synergistic effect that increases assay sensitivity in ELISA Recognizes a linear epitope - binding is not conformation-dependent Sensitivity of approx. 300 pg/mL in ELISA Does not cross-react with SARS-CoV, HCoV-229E, HCoV-HKU1, HCoV-NL63 and HCoV-OC43
Cat# 9550
MAb to SARS-CoV-2 S1 (Trimeric)
Cat# 9551
MAb to SARS-CoV-2 S1 (Trimeric)
Cat# 9565
MAb to SARS-CoV-2 S1 (Trimeric)
*9551 and 9565 can be used together in a 1:1 ratio to increase assay sensitivity in ELISA
SYNERGISTIC EFFECT OF CAT# 9551 AND 9565 ENHANCES DETECTION OF SARS-COV-2 TRIMERIC SPIKE PROTEIN
MAb sandwich ELISAs were compared for their ability to detect recombinant SARS-CoV-2 across 4 serial dilutions (10 ng/mL – 0.3 ng/mL). One assay used Cat# 9550 as the capture and Cat# 9551 as the detection antibody a second assay used Cat# 9550 as the capture and Cat# 9565 as the detection antibody and a third assays used Cat#9550 as the capture and Cat#9551 and Cat#9565 together in a 1:1 ratio as the detection antibodies. Using Cat# 9551 and 9565 in combination creates a synergistic effect that results in an enhanced detection of SARS-CoV-2 Trimeric Spike protein across all concentrations.
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