Detect down to 1 copy DNA in presence of inhibitors
Activity of Lyo-Ready Genotyping Direct qPCR FFPE in liquid format ( blue ) and lyophilized ( red ) in a qPCR assay, using a 10-fold serial dilution of synthetic DNA (1,000, 100, 10 and 1 copies respectively), in the presence of 20% MDX168 Extraction Buffer. The results illustrate that the lyophilized Lyo-Ready Genotyping Direct qPCR FFPE retains the ability to efficiently amplify to the same level as the liquid mix with the same level of sensitivity and reproducibility.
100 copy 10 copy 1,000 copy
1 copy
Liquid | Lyophilized
Lyophilized mixes maintain a stable shelf life for up to 18 months
A)
Allele 1
Allele 2
Allele 1/2
B)
Lyophilized
Liquid
RFU for Allele 1
RFU for Allele 1
Lyo-Ready Genotyping Direct qPCR FFPE was lyophilized, and its stability tested in an accelerated stability study. The lyophilized mix ( red ) was incubated at 37°C for 1 month and tested against the fresh liquid mix ( blue ) in a standard dual-labelled probe genotyping assay in the presence of 20% MDX168 Extraction Buffer. Results show that the lyophilized mix retain A) the Ct-values and end-fluorescence in real-time PCR and B) allelic discrimination as the liquid mix following accelerated stability tests with projected 18-months stability at ambient temperature.
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