Immunoassay Blocking Agents: Reducing False Results

Mouse IgG Blocker & Mouse Serum

Neutralizing HAMA for Cleaner Results Mouse IgG blocker is a highly purified immunoglobulin designed to block human anti-mouse antibodies (HAMA), which are a common source of interference in assays using mouse monoclonal antibodies. It binds to HAMA in the sample, preventing it from interacting with assay antibodies. Mouse serum, on the other hand, offers a broader blocking profile. It contains a natural mix of mouse proteins, including immunoglobulins, making it ideal for assays where multiple types of interference may be present.

WHEN TO USE EACH

BENEFITS

• Reduces false positives and false negatives • Improves assay reproducibility • Compatible with ELISA, CLIA, and LFA formats

Mouse IgG Blocker

Mouse Serum

For targeted HAMA interference in high- sensitivity assays

For broader interference blocking in early-stage assay development or when multiple interferences are suspected

ORDERING INFORMATION Mouse Serum

Cat. No.

Cat. No. A66181M A66185M

Mouse IgG

88131

Heat Inactivated

22—28 mg/mL 50—55 mg/mL

N14010M

Normal

A66185M-NA

50—55 mg/mL, Azide Free

A66186M

9—13 mg/mL

A66186M-NA

9—13 mg/mL, Azide Free

A66187M A66189M

10—20 mg/mL

45—55 mg/mL, Proclin 30—40 mg/mL, Azide Free

A66190M-NA

WHEN TO USE BLOCKERS: ASSAY FORMATS AT RISK OF INTERFERENCE

SEROLOGY (IgM, IgG, IgA)

SANDWICH ASSAY

COMPETITIVE ASSAY

Detector Labels

Inhibitor Antigens Patient Analytes Detector Labels

Secondary Antibody

Detector Labels

Detection Antibodies

Patient’s IgG Ab Patient’s IgM Ab

Antigens

Capture Antibodies

Antigens

DIRECT DETECTION

INDIRECT DETECTION

IgG DETECTION

IgM DETECTION

DIRECT DETECTION