Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety.
All-in-One dUTP qPCR Master Mix Optimized for High-Throughput & Multiplex Assays Inhibitor-Tolerant | Prevents Carryover Contamination | Lyophilization-Ready
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Stronger Performance. Proven. Independent studies show Meridian’s mixes outperform Roche, Thermo, and QuantaBio in real-world conditions.
Meridian’s Lyo-Ready AOF dUTP DNA qPCR and RT-qPCR high-concentration master mixes are engineered for superior performance, preventing carryover contamination and minimizing false positives. With exceptional inhibitor tolerance, they enable ultra-sensitive amplification from crude lysates or purified nucleic acids derived from blood, saliva, and urine samples. Their optimized formulation ensures accurate, reliable results, making them ideal for high-throughput and multiplex assays. BSA-free and lyophilization-ready, these master mixes offer a cost-effective, room-temperature stable solution, reducing reliance on animal-sourced reagents while enhancing efficiency and consistency.
Highly sensitive detection of RNA/DNA from purified or crude samples, including blood, saliva, and urine. Ready-to-use, glycerol-free qPCR master mixes can be used as a liquid or lyophilized for ambient-temperature stable assays. Incorporates dUTP to prevent carryover contamination and reduce false positives, making it ideal for high-throughput and multiplex assays
Compared to leading competitors, Meridian’s qPCR master mixes deliver: • Ready-to-use master mix providing a complete solution to prevent PCR inhibition, eliminate carryover contamination, and enable ambient-temperature assay stabilization • Consistently earlier Ct values with inhibitor-rich samples, ensuring higher sensitivity • Superior multiplexing efficiency , enabling detection of multiple pathogens with higher reproducibility. • Unmatched flexibility with liquid and lyophilized formats that maintain equivalent high performance. • Cost-effective solutions that enhance operational efficiency and reduce reagent waste. Why Choose Meridian’s All-in-One Inhibitor-Tolerant dUTP qPCR Master Mixes?
Blood Specific Mixes: Optimized for Specific Clinical Samples:
Ideal for detection of low-copy number bacterial and viral pathogens, and cell-free nucleic acids. PRODUCT CAT No. VOLUME
REACTIONS 200 Rxns 1,000 Rxns 10,000 Rxns 200 Rxns 1,000 Rxns 10,000 Rxns
1 mL 5 mL
Lyo-Ready AOF dUTP DNA qPCR Blood Mix, 4x
MDX322
50 mL
1 mL 5 mL
Lyo-Ready AOF dUTP RNA/DNA qPCR Blood Mix, 4x
MDX323
50 mL
A) Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322) B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323) Enhanced Performance in Anticoagulant-Stabilized Blood (K2-EDTA, Sodium Heparin, and Sodium Citrate)
EDTA | Heparin | Citrate
Anticoagulants and high concentrations of whole blood are known to inhibit efficiency of qPCR reactions. Whole human blood at concentrations of 2%, 5%, and 10% were tested in the presence of K2-EDTA ( orange ), sodium heparin ( green ), and sodium citrate ( grey ). These tests were conducted using A) Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322) and compared with Roche KAPA Probe Force qPCR Master Mix and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix and B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323) and compared with Quantabio UltraPlex ™ 1-Step ToughMix ® , and ThermoFisher TaqPath ™ 1-Step Multiplex Master Mix. The results demonstrate that Lyo-Ready AOF dUTP Blood mixes maintain higher performance and consistently earlier Ct values in the presence of inhibitors in high concentrations of blood containing common anticoagulants, ensuring reliable amplification even under challenging conditions.
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A) Lyo-Ready AOF dUTP DNA qPCR Blood with 10% EDTA Plasma Mycobacterium tuberculosis Plasmodium malariae Superior Multiplex Performance in Inhibitor-Rich Blood Samples
Salmonella typhimurium
B) Lyo-Ready AOF dUTP DNA qPCR Blood with 10% Serum Staphylococcus Aureus
Plasmodium falciparum
Epstein-Barr Virus
MDX322 | KAPA Probe Force | TaqPath | PerfeCTa
A) Targets for three pathogens (Mycobacterium tuberculosis, Plasmodium malariae, and Salmonella typhimurium) in the presence of 10% EDTA Plasma and B) three different pathogens ( Staphylococcus Aureus, Plasmodium falciparum , and Epstein-Barr Virus) in the presence of 10% serum were amplified in a triplex reaction using lyophilized Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322) ( red ), Roche KAPA Probe Force qPCR Master Mix ( green ), Thermofisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ), or QuantaBio PerfeCTa ® MultiPlex qPCR ToughMix ( grey ). The results illustrate that Lyo-Ready AOF dUTP DNA qPCR Blood has higher multiplexing capabilities, greater inhibitor tolerance and better reproducibility than other suppliers’ inhibitor tolerant mixes across a range of pathogen types.
Enhanced Multiplexing, Inhibitor Tolerance, and Reproducibility
A) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood with 5% Whole Blood EDTA with blood-borne viral pathogens Hepatitis B virus (HBV) Hepatitis C virus (HCV)
Human Immunodeficiency virus (HIV)
B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood with 5% Whole Blood EDTA with Tropical Viral Pathogens Chikungunya virus (CHIKV) West Nile virus (WNV) Dengue virus (DENV)
Zika virus (ZIKV)
MDX323 | TaqPath | UltraPlex
Targets for A) three blood-borne pathogens (Hepatitis B virus (HBV), Hepatitis C virus (HCV), and Human Immunodeficiency virus (HIV)) and B) four tropical pathogens (Chikungunya virus (CHIKV), West Nile virus (WNV), Dengue virus (DENV) and Zika virus (ZIKV)) in the presence of 5% whole blood EDTA were amplified in a triplex or quadruplex reaction using lyophilized Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323) ( red ), Thermofisher TaqPath ™ 1-Step Multiplex Master Mix ( black ), or QuantaBio UltraPlex ™ 1-Step ToughMix ( grey ). The results illustrate that Lyo-Ready AOF dUTP RNA/DNA qPCR Blood has higher multiplexing capabilities, greater inhibitor tolerance and better reproducibility than other suppliers’ inhibitor tolerant mixes across a range of pathogen types.
Equivalent High Sensitivity and Reproducibility in Liquid and Lyophilized Formats
A) Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322)
B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323)
10,000 copies
10,000 copies
1,000 copies
1,000 copies
100 copies
100 copies
10 copies 1 copy
10 copies
Liquid | Lyophilized
A) Lyo-Ready AOF dUTP DNA qPCR Blood was used to amplify a 10-fold serial dilution of synthetic DNA target (10,000, 1,000, 100, 10, and 1 copy, respectively) in the presence of 20% Serum and B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood was used to amplify a 10-fold serial dilution of synthetic RNA target (10,000, 1,000, 100 and 10 copies, respectively) in the presence of 5% EDTA Blood, in liquid ( blue ) and lyophilized ( red ) format. The results illustrate that the lyophilized mixes maintain equivalent application efficiency as the liquid mix, with high sensitivity and reproducibility.
Saliva Specific Mixes:
Optimized for robust RNA detection in saliva samples, suitable for pathogens including M. tuberculosis, M. pneumoniae , Adenovirus, Cytomegalovirus, Influenza A and RSV. PRODUCT CAT No. VOLUME REACTIONS
1 mL 5 mL
200 Rxns 1,000 Rxns 10,000 Rxns 200 Rxns 1,000 Rxns 10,000 Rxns
Lyo-Ready AOF dUTP DNA qPCR Saliva Mix, 4x
MDX332
50 mL
1 mL 5 mL
Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva Mix, 4x
MDX333
50 mL
Lyo-Ready AOF dUTP DNA qPCR Saliva (MDX332) Mycobacterium tuberculosis Superior Multiplexing in Respiratory Diagnostics Adenovirus
Four respiratory pathogens ( Mycobacterium tuberculosis , Adenovirus, Cytomegalovirus and Mycoplasma pneumoniae ) were amplified in a quadruplex reaction in the presence of 30% artificial sputum using Lyo-Ready AOF dUTP DNA qPCR Saliva in lyophilized format ( red ) and compared to Quantabio ToughMix PCR Master Mix ( grey ), Promega GoTaq Enviro qPCR Master Mix ( orange ) and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ). Results demonstrate that Lyo-Ready AOF dUTP DNA qPCR Saliva has higher multiplexing capabilities, inhibitor tolerance and better reproducibility than other supplier’s qPCR mixes for respiratory targets.
Cytomegalovirus
Mycoplasma pneumoniae
MDX332 | ToughMix | GoTaq | TaqPath
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Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva (MDX333) Influenza A RSV A
Adenovirus
MDX333 | Reliance One-Step | DuraPlex
Three viral targets (Influenza A, RSV A and Adenovirus) were amplified in a triplex reaction using Lyo-Ready Direct RNA/DNA qPCR Saliva in lyophilized format ( red ) or Reliance One-Step Multiplex RT-qPCR Supermix ( purple ) or TaqPath ™ DuraPlex ™ 1-Step RT-qPCR Master Mix ( black ) in the presence of 50% UTM swab (UTM dipped with artificial sputum swab). The results illustrate that dry Lyo-Ready Direct RNA/DNA qPCR Saliva has higher multiplexing capabilities, inhibitor tolerance and better reproducibility than other supplier’s inhibitor tolerant mixes.
Consistent High-Efficiency Amplification in Liquid and Lyophilized Formats
A) Lyo-Ready AOF dUTP DNA qPCR Saliva (MDX332)
B) Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva (MDX333)
1,000 copies 100 copies
1 copy 10 copies
Liquid | Lyophilized
Lyo-Ready AOF dUTP DNA qPCR Saliva in liquid (blue) and lyophilized (red) format was compared in the ability to amplify a 10-fold synthetic DNA target (1,000, 100, 10 and 1 copies respectively) in the presence of 20% artificial sputum. The results illustrate that the lyophilized mix maintains the same high application efficiency as the liquid mix, with high sensitivity and reproducibility. A) Lyo-Ready AOF dUTP DNA qPCR Saliva was used to amplify a 10-fold serial dilution of synthetic DNA target (10,000, 1,000, 100, 10, and 1 copy, respectively) in the presence of 20% artificial sputum and B) Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva was used to amplify a 10-fold serial dilution of synthetic RNA target (10,000, 1,000, 100 and 10 copies, respectively) in the presence of 5% artificial sputum, in liquid (blue) and lyophilized (red) format. The results illustrate that the lyophilized mixes maintain the same high application efficiency as the liquid mix, with high sensitivity and reproducibility.
No Cold Chain? No Problem.
Lyophilization-ready master mixes provide ambient-temperature stability, reducing shipping and storage costs.
Urine Specific Mixes:
Perfect for detecting sexually transmitted pathogens such as M. genitalium, T. pallidum, C. trachomatis and N. gonorrhoeae and tumor-related biomarkers such as IGFBP5, CDC2 and MDK. PRODUCT CAT No. VOLUME REACTIONS
1 mL 5 mL
200 Rxns 1,000 Rxns 10,000 Rxns 200 Rxns 1,000 Rxns 10,000 Rxns
Lyo-Ready AOF dUTP DNA qPCR Urine Mix, 4x
MDX352
50 mL
1 mL 5 mL
Lyo-Ready AOF dUTP RNA/DNA qPCR Urine Mix, 4x
MDX353
50 mL
Lyo-Ready AOF dUTP DNA qPCR Urine (MDX352) Mycoplasma genitalium Robust Multiplex qPCR in Crude Urine Samples
Treponema pallidum
Chlamydia trachomatis
Neisseria gonorrhoeae
MDX352 | ToughMix | KAPA | TaqPath
Four sexually transmitted pathogens ( Mycoplasma genitalium, Treponema pallidum (Syphilis), Chlamydia trachomatis and Neisseria gonorrhoeae ) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready AOF dUTP DNA qPCR Urine in lyophilized format ( red ) and compared to Quantabio ToughMix PCR Master Mix ( grey ), Roche KAPA Probe Force qPCR kit ( green ) and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ). Results demonstrate that Lyo-Ready AOF dUTP DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.
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Precision Multiplexing for Reliable Urinary Cancer Biomarker Detection
Lyo-Ready AOF dUTP RNA/DNA qPCR Urine (MDX353)
IGFBP5
CDC2
MDK
MDX353 | DuraPlex | UltraPlex
Three tumor-related mRNA markers (IGFBP5, CDC2 and MDK) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready AOF dUTP RNA/DNA qPCR Urine in lyophilized format ( red ) and compared to ThermoFisher TaqPath ™ DuraPlex ™ 1-Step RT-qPCR Master Mix ( black ) and Quantabio UltraPlex 1-Step ToughMix ( grey ). Results demonstrate that Lyo-Ready AOF dUTP RNA/DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.
Equivalent High-Sensitivity Amplification of Low-Copy Targets in Liquid and Lyophilized Formats
A) Lyo-Ready AOF dUTP DNA qPCR urine
B) Lyo-Ready AOF dUTP RNA/DNA qPCR urine
Activity of A) Lyo-Ready AOF dUTP DNA qPCR urine in lyophilized ( red ) and liquid ( blue ) format was compared in the ability to amplify a 10-fold serial dilution of synthetic DNA (1,000, 100, 10 and 1 copies respectively) and B) Activity of Lyo-Ready AOF dUTP RNA/DNA qPCR urine in lyophilized ( red ) and wet ( blue ) format was compared in the ability to amplify a 10-fold synthetic RNA target (1,000, 100, 10 and 1 copies respectively) in the presence of 10% human urine. The results illustrate that the dried mix retains the ability to efficiently amplified to the same level as the wet mix with the same level of sensitivity and reproducibility for both.
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
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