Meridian All-in-One Lyo-Ready dUTP qPCR Master Mixes

Compared to leading competitors, Meridian’s qPCR master mixes deliver: • Ready-to-use master mix providing a complete solution to prevent PCR inhibition, eliminate carryover contamination, and enable ambient-temperature assay stabilization • Consistently earlier Ct values with inhibitor-rich samples, ensuring higher sensitivity • Superior multiplexing efficiency , enabling detection of multiple pathogens with higher reproducibility. • Unmatched flexibility with liquid and lyophilized formats that maintain equivalent high performance. • Cost-effective solutions that enhance operational efficiency and reduce reagent waste. Why Choose Meridian’s All-in-One Inhibitor-Tolerant dUTP qPCR Master Mixes?

Blood Specific Mixes: Optimized for Specific Clinical Samples:

Ideal for detection of low-copy number bacterial and viral pathogens, and cell-free nucleic acids. PRODUCT CAT No. VOLUME

REACTIONS 200 Rxns 1,000 Rxns 10,000 Rxns 200 Rxns 1,000 Rxns 10,000 Rxns

1 mL 5 mL

Lyo-Ready AOF dUTP DNA qPCR Blood Mix, 4x

MDX322

50 mL

1 mL 5 mL

Lyo-Ready AOF dUTP RNA/DNA qPCR Blood Mix, 4x

MDX323

50 mL

A) Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322) B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323) Enhanced Performance in Anticoagulant-Stabilized Blood (K2-EDTA, Sodium Heparin, and Sodium Citrate)

EDTA | Heparin | Citrate

Anticoagulants and high concentrations of whole blood are known to inhibit efficiency of qPCR reactions. Whole human blood at concentrations of 2%, 5%, and 10% were tested in the presence of K2-EDTA ( orange ), sodium heparin ( green ), and sodium citrate ( grey ). These tests were conducted using A) Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322) and compared with Roche KAPA Probe Force qPCR Master Mix and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix and B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323) and compared with Quantabio UltraPlex ™ 1-Step ToughMix ® , and ThermoFisher TaqPath ™ 1-Step Multiplex Master Mix. The results demonstrate that Lyo-Ready AOF dUTP Blood mixes maintain higher performance and consistently earlier Ct values in the presence of inhibitors in high concentrations of blood containing common anticoagulants, ensuring reliable amplification even under challenging conditions.