A) Lyo-Ready AOF dUTP DNA qPCR Blood with 10% EDTA Plasma Mycobacterium tuberculosis Plasmodium malariae Superior Multiplex Performance in Inhibitor-Rich Blood Samples
Salmonella typhimurium
B) Lyo-Ready AOF dUTP DNA qPCR Blood with 10% Serum Staphylococcus Aureus
Plasmodium falciparum
Epstein-Barr Virus
MDX322 | KAPA Probe Force | TaqPath | PerfeCTa
A) Targets for three pathogens (Mycobacterium tuberculosis, Plasmodium malariae, and Salmonella typhimurium) in the presence of 10% EDTA Plasma and B) three different pathogens ( Staphylococcus Aureus, Plasmodium falciparum , and Epstein-Barr Virus) in the presence of 10% serum were amplified in a triplex reaction using lyophilized Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322) ( red ), Roche KAPA Probe Force qPCR Master Mix ( green ), Thermofisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ), or QuantaBio PerfeCTa ® MultiPlex qPCR ToughMix ( grey ). The results illustrate that Lyo-Ready AOF dUTP DNA qPCR Blood has higher multiplexing capabilities, greater inhibitor tolerance and better reproducibility than other suppliers’ inhibitor tolerant mixes across a range of pathogen types.
Enhanced Multiplexing, Inhibitor Tolerance, and Reproducibility
A) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood with 5% Whole Blood EDTA with blood-borne viral pathogens Hepatitis B virus (HBV) Hepatitis C virus (HCV)
Human Immunodeficiency virus (HIV)
B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood with 5% Whole Blood EDTA with Tropical Viral Pathogens Chikungunya virus (CHIKV) West Nile virus (WNV) Dengue virus (DENV)
Zika virus (ZIKV)
MDX323 | TaqPath | UltraPlex
Targets for A) three blood-borne pathogens (Hepatitis B virus (HBV), Hepatitis C virus (HCV), and Human Immunodeficiency virus (HIV)) and B) four tropical pathogens (Chikungunya virus (CHIKV), West Nile virus (WNV), Dengue virus (DENV) and Zika virus (ZIKV)) in the presence of 5% whole blood EDTA were amplified in a triplex or quadruplex reaction using lyophilized Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323) ( red ), Thermofisher TaqPath ™ 1-Step Multiplex Master Mix ( black ), or QuantaBio UltraPlex ™ 1-Step ToughMix ( grey ). The results illustrate that Lyo-Ready AOF dUTP RNA/DNA qPCR Blood has higher multiplexing capabilities, greater inhibitor tolerance and better reproducibility than other suppliers’ inhibitor tolerant mixes across a range of pathogen types.
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