Equivalent High Sensitivity and Reproducibility in Liquid and Lyophilized Formats
A) Lyo-Ready AOF dUTP DNA qPCR Blood (MDX322)
B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood (MDX323)
10,000 copies
10,000 copies
1,000 copies
1,000 copies
100 copies
100 copies
10 copies 1 copy
10 copies
Liquid | Lyophilized
A) Lyo-Ready AOF dUTP DNA qPCR Blood was used to amplify a 10-fold serial dilution of synthetic DNA target (10,000, 1,000, 100, 10, and 1 copy, respectively) in the presence of 20% Serum and B) Lyo-Ready AOF dUTP RNA/DNA qPCR Blood was used to amplify a 10-fold serial dilution of synthetic RNA target (10,000, 1,000, 100 and 10 copies, respectively) in the presence of 5% EDTA Blood, in liquid ( blue ) and lyophilized ( red ) format. The results illustrate that the lyophilized mixes maintain equivalent application efficiency as the liquid mix, with high sensitivity and reproducibility.
Saliva Specific Mixes:
Optimized for robust RNA detection in saliva samples, suitable for pathogens including M. tuberculosis, M. pneumoniae , Adenovirus, Cytomegalovirus, Influenza A and RSV. PRODUCT CAT No. VOLUME REACTIONS
1 mL 5 mL
200 Rxns 1,000 Rxns 10,000 Rxns 200 Rxns 1,000 Rxns 10,000 Rxns
Lyo-Ready AOF dUTP DNA qPCR Saliva Mix, 4x
MDX332
50 mL
1 mL 5 mL
Lyo-Ready AOF dUTP RNA/DNA qPCR Saliva Mix, 4x
MDX333
50 mL
Lyo-Ready AOF dUTP DNA qPCR Saliva (MDX332) Mycobacterium tuberculosis Superior Multiplexing in Respiratory Diagnostics Adenovirus
Four respiratory pathogens ( Mycobacterium tuberculosis , Adenovirus, Cytomegalovirus and Mycoplasma pneumoniae ) were amplified in a quadruplex reaction in the presence of 30% artificial sputum using Lyo-Ready AOF dUTP DNA qPCR Saliva in lyophilized format ( red ) and compared to Quantabio ToughMix PCR Master Mix ( grey ), Promega GoTaq Enviro qPCR Master Mix ( orange ) and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ). Results demonstrate that Lyo-Ready AOF dUTP DNA qPCR Saliva has higher multiplexing capabilities, inhibitor tolerance and better reproducibility than other supplier’s qPCR mixes for respiratory targets.
Cytomegalovirus
Mycoplasma pneumoniae
MDX332 | ToughMix | GoTaq | TaqPath
www.meridianbioscience.com/lifescience
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