African Swine Fever Virus (ASFV)

African Swine Fever Virus (ASFV)

An outbreak of African Swine Fever Virus (ASFV) is ravaging Asia’s pig industry and continuing to spread globally. The outbreak began in China in August 2018 and since the initial infection, the country has lost 22% of its pig herd and is predicted to lose up to 50%, representing the largest livestock loss in modern history. The disease has spread to neighboring countries including Mongolia, Russia, Cambodia and Vietnam and there is a high risk of it spreading throughout Europe and to the USA. ASFV is a DNA virus that is transmitted through direct contact with an infected pig, contaminated feed or through the bite of an infected tick. Virus isolates can vary in virulence from highly pathogenic strains that cause acute disease and high mortality (90-100%) to low–virulence isolates that present similar but less intense symptoms making the disease chronic and difficult to diagnose. There is no vaccine or treatment. The genome of ASFV is very large and complex, consisting of over 150 genes with variability in expression, leading to 22 different genotypes of ASFV. Several immunogenic viral antigens have been identified, including p30 which is one of the most antigenic structural proteins involved in ASFV entry and is expressed very early on in infection (2-4 hours post-infection) (Zhang

et. al., 2017) . Research has shown that with low-virulence strains, virus shedding and antibodies to the virus can persist for months after infection. ASFV can be diagnosed by virus isolation, ELISA, immunofluorescence or PCR. Clinical samples used for testing are lymph nodes, kidneys, spleen, lung, blood and serum. The simultaneous detection of both antigens and antibodies is recommended in order to identify the presence of potentially chronic infections that continue to spread the disease.

Zhang, J. et al. (2017). Roles of African Swine Fever Virus structural proteins in viral infection. J. Vet Res., 61, 135-143.

Monoclonal Antibodies to ASFV MAb to ASFV p30 Detects the early-expressed p30 structural antigen. Suitable for ELISA, IFA, IHC, IP and WB.

Recombinant Antigens to ASFV

Produced in insect cells and affinity purified by proprietary chromatography. ASFV p30 Cat# R01793 ASFV p30 Cat# R01795 ASFV p54 Cat# R01796

Electron microscopy image of the extracellular African Swine Virus particle. The ASFV virion is composed of different concentric layers: the external envelope (red), the viral capsid (green), the inner envelope (yellow), the core shell (blue), and

the nucleoid (purple). Zhang, J. et al., (2017)

Cat# C01881M Cat# C01882M

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Molecular reagents

PRODUCT

CATALOG #

DESCRIPTION

ENZYMES Low DNA Taq HS 5 U/μL Low DNA Taq HS 10 U/μL

MDX009 MDX010

Heat-activated, thermostable DNA polymerase with low residual DNA content. Ideal for multiplex assays involving amplification of bacterial and fungal DNA. A high concentration (50U/ml), lyophilization-compatible Taq DNA polymerase containing a DNA aptamer which binds reversibly to the polymerase. Suitable for developing highly specific, high-throughput assays. Designed for multiplex assays detecting microbial or fungal DNA. Incorporates a heat-activated DNA polymerase with low residual DNA content. Ideal for multiplex assays requiring sensitive detection of DNA targets in inhibitor-rich samples. Contains an antibody-mediated hot-start polymerase. Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Contains an antibody-mediated hot-start polymerase.

Aptamer Taq HS (Glycerol-Free)

MDX015

OPTMIZED MASTER MIXES

Low DNA qPCR Mix

MDX030

Fast qPCR Mix

MDX020

Inhibitor-Tolerant qPCR Mix

MDX013

COMPANION REAGENTS Tissue Extract-PCR Buffers

MDX004

Lysis and neutralization buffer optimized for use with Taq HS DNA.

Fast qPCR Buffer, 4x

MDX033

Optimized for use with Taq HS DNA Polymerase (MDX008) .

Optimized for use with Taq HS DNA Polymerase (MDX008) and RNase-Tolerant MMLV-RT (MDX043) . Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays. A mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity at room temperature. For use in hot-start PCR.

1-Step RT-qPCR Buffer, 4x

MDX034

RNase Inhibitor

MDX056

Taq HS Antibody

MDX014

Product Reference Chart

Master Mix Hot-Start

DNA Detection

RNA Detection

Multiplex Reactions

High-Sensitivity (Low copy target)

Inhibitor-Rich Samples

Room-Temperature Assay Set-Up

Product Name

Cat#

MDX001

Taq DNA Polymerase

No

Taq DNA HS Polymerase

MDX008

Antibody

MDX015

Aptamer Taq HS

Aptamer

MDX009 & MDX010

Low DNA Taq HS

Chemical

MDX020

Fast qPCR Mix

Antibody

Inhibitor-Tolerant qPCR Mix

MDX013

Antibody

MDX030

Low DNA qPCR Mix

Antibody

• Suitable | ••

Recommended

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