Cardiac & Metabolic Markers Catalog

Thrombomodulin

Troponin C-Cardiac (cTnC) cTnC is part of the Troponin Complex and is responsible for binding calcium to activate muscle contraction. In cardiac muscle, cTnC binds to Cardiac Troponin I (cTnI) and Cardiac Troponin T (cTnT), whereas cTnC binds to slow Skeletal Troponin I (ssTnI) and Troponin T (ssTnT) in slow-twitch skeletal muscle. Antigens A86857H • Native antigen from human heart tissue • > 98% pure (SDS-PAGE), lyophilized • Suitable for use as a standard, immunogen for antisera production, tracer for iodination Troponin I-Cardiac (cTnI) As part of the troponin complex, cTnI binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place and is present in cardiac muscle tissue in a single isoform. cTnI has several phosphorylation sites and the pattern of phosphorylation changes in response to disease. Phosphorylated cTnI changes the conformation of the protein and modifies its interaction with other troponins and alters the myofilament response to calcium. Assays for Cardiac Troponins I and T (cTnI and cTnT) have become widely accepted tools for diagnosing acute myocardial infarction. Antibody Pairs • Suitable for use in ELISA, LF • Recombinant MAbs produced in HEK specific for Troponin I-cardiac protein CAPTURE DETECTION BN1148 BN1149 * New recombinant antibodies enabling highly sensitive cTnI detection Monoclonals • Suitable for use in ELISA and WB • Reacts equally with free cardiac Troponin I (cTnI) and Complexed cTnI (unless otherwise noted) • Not affected by phosphorylation • Produced in vivo EPITOPE BINDING H01250M a.a. 1-15 * Also works in IHC H86008M a.a. 18-28 * Also works in IHC H01247M a.a. 148-158 * Also works in IHC, reacts mostly with free cTnI (not complexed) H86267M a.a. 169-178 H86550M a.a. 186-192 H86004M a.a. 190-196 * Also works in IHC H86307M a.a. 25-40 H01340M a.a. 41-49

Tissue Plasminogen Activator (tPA) tPA is a serine protease that catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown. An elevated level of tPA antigen is a proposed marker of increased coronary risk. Polyclonals CATALOG SOURCE FORMAT APPLICATION K63250R Rabbit Purified EIA,IHC,WB Thrombomodulin is a protein present in the endothelial membrane of cells which serves as a co-factor to protein C in the coagulation cascade. Its functions as an anti-coagulant regulator, and can be measured as a soluble protein in the serum after vascular injury. Antigens R01802 • Human Thrombomodulin (THBD) Protein, Recombinant (His-tag C-terminus) • Source: HEK293) • ≥ 90% (SDS-PAGE) in phosphate buffer saline pH 7.2 • Suitable for ELISA, lateral flow, and western blot

Troponin Complex

Troponin is a complex of three regulatory proteins (Troponin C, Troponin I, and Troponin T) that is integral to muscle contraction in skeletal muscle and cardiac muscle, but not smooth muscle. Troponin I and T are specific to cardiac muscles and are very sensitive and specific markers for unstable angina and myocardial infarction. Troponin C is associated with both cardiac and skeletal muscles and is not used in the diagnosis of myocardial damage. Monoclonals CATALOG SOURCE FORMAT APPLICATION BN1147 Mouse Purified ELISA, LF

Antigens A86608H

• Native antigen from human heart tissue • >95% pure (SDS-PAGE) • Native antigen from human heart tissue • Complex of Troponin I-T-C in a molar ratio 1:1:1

A86111H