Veterinary & Animal Health

Molecular and Immunoassay solutions for infectious disease testing

Veterinary & Animal Health Molecular and Immunoassay solutions for infectious disease testing

Veterinary medicine has transformed the health and welfare of livestock and companion animals worldwide. As the globalized trade of animals and their products increases, the threat of infectious disease impacting livestock health and food supplies has also increased in addition to the emergence of zoonotic diseases. Some infectious diseases have highly visible consequences, while others remain silent for weeks or months. Accurate and rapid diagnostic tests are therefore an essential component to detect, control and eradicate such diseases. With so many different species and diseases in veterinary heath, vet diagnostic tests rely on both immunoassay and molecular technologies. qPCR has become an indispensable component of routine veterinary diagnostics due to its speed, sensitivity and selectivity, and potential for multiplex analysis. However, there is a growing need for field-based point-of-care testing to enable a rapid diagnosis and prevent transmission, especially when animals live in flocks and herds. Transport of specimens from the field to the laboratory can be a lengthy process that can delay critical decision-making and severely affect the quality of the samples. By using lateral point-of-care devices or ambient temperature stable qPCR assays, the need for transporting specimens is reduced as assays can be performed on-site in low-resource settings such as farms and in the wild.

Optimized qPCR & RT-qPCR Mixes for Whole Blood, Urine and other Inhibitor-Rich Clinical Samples

100 High qPCR Reaction Efficiencies Across a Range of Crude Sample Types

Inhibitor-Tolerant qPCR Mix ( MDX013 ) was used in a series of qPCR reactions containing a variety of crude clinical samples including whole blood, cerebrospinal fluid (20%) and urine (20%). The results demonstrate that the reaction efficiency of the Inhibitor- Tolerant qPCR Mix remained within 90-110% in the presence of a wide range of common PCR inhibitors.

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Types of qPCR/RT-qPCR Optimized Mixes

Inhibitor-Tolerant Mixes Optimized for extraction- free assays from crude clinical specimens (sputum, saliva and stool)

Fast Mixes Ideal for multiplex assays on fast, automated high- throughput systems

Lyo-ready Mixes Pre-formulated with lyo-excipients for lyophilization into beads or cakes

Air-Dryable Mixes Ready-to-use mix

compatible with a range of air-drying protocols to produce an ambient temperature stable assay

Features

MDX021 MDX023 MDX024 2x

MDX082 MDX092 MDX095 4x

MDX013 MDX016

MDX020 MDX032

Cat#

Concentration

Master Mix

Antibody

Hot-Start

Ambient- Temperature Assays

Multiplex Reactions

RNA/DNA Extraction-Free Protocols

Inhibitor-Tolerant

RT-qPCR Extraction Control • Monitors assay inhibition

VLP RNA Extraction Control

• Closely mimics the test sample • Undergoes the same processing from lysis and extraction to detection • Compatible with lyophilization for creating freeze-dried mixes

• Suitable for use with inhibitor-rich samples

MDX068 (Red, Cy5) MDX069 (Orange, HEX) MDX071 (Custom)

MDX028 (Red, Quasar 670) MDX029 (Orange, Cal Fluor Orange)

Recommended

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Molecular Product Selection Chart

• Suitable •• Recommended

              

••

Low DNA qPCR Mix

MDX030

Chemical

•• ••

••

Fast qPCR Mix

MDX020

Antibody

MDX031 & MDX060

• ••

Hi-Throughput dUTP Mix

Antibody

•• ••

Inhibitor-Tolerant qPCR Mix

MDX013

Antibody

•• •• •• •• ••

Inhibitor-Tolerant RT-qPCR Mix

MDX016

Antibody

•• ••

Fast 1-Step RT-qPCR Mix

MDX032

Antibody

•• •• ••

Low LOD 1-Step RT-qPCR Mix

MDX025

Antibody

••

• •

Lyo-Compatible MMLV-RT

MDX042

• ••

RNase-Tolerant MMLV-RT

MDX043

• •

MMLV-RT

MDX044

••

•• ••

•

Air-Dryable ™ qPCR Mix

MDX082

Antibody

••

•• •• • •

Air-Dryable ™ 1-Step RT-qPCR Mix

MDX095

Antibody

••

•• •• •• •

Air-Dryable ™ Direct DNA qPCR Blood

MDX092

Antibody

MDX021 & MDX023

••

•• ••

•

Lyo-Ready ™ qPCR Mix

Antibody

••

• ••

Lyo-Ready ™ 1-Step RT-qPCR Mix

MDX024

Antibody

••

• ••

Lyo-Ready ™ 1-Step RT-qPCR Virus Mix

MDX062

Antibody

••

•• ••

Lyo-Ready ™ LAMP Mix

MDX097

••

•• ••

Lyo-Ready ™ RT-LAMP 1-Step Mix

MDX108

••

Bst DNA Polymerase

MDX012

••

•

High Conc. Glycerol-Free Bst

MDX018

• • •

Taq DNA Polymerase

MDX001

•• •• •• ••

Taq HS DNA Polymerase

MDX008

Antibody

••

•• •

Glycerol-Free Taq HS

MDX011

Antibody

••

•• ••

••

Aptamer Taq HS (Glycerol-Free)

MDX015

Aptamer

MDX009 & MDX010

••

Low DNA Taq HS

Chemical

••

High-Fidelity Pfu

MDX003

••

•• •• www.MeridianLifeScience.com

High-Specificity Pfu HS

MDX006

Aptamer

Optimized Mixes

PRODUCT

CAT NO.

VOLUME

REACTIONS

qPCR (DNA Template) Hot-Start Master Mixes

5 mL

500 Rxn

Low DNA qPCR Mix Ideal for microbial and fungal DNA detection assays.

MDX030

100 mL

10,000 Rxn

5 mL

500 Rxn

Fast qPCR Mix Suitable for fast high-multiplex qPCR assays.

MDX020

100 mL

10,000 Rxn

2 mL

500 Rxn

Fast qPCR Mix, 5x Suitable for fast high-multiplex qPCR assays (5x concentration).

MDX072

40 mL

10,000 Rxn

2 mL

500 Rxn

Digital PCR Mix, 5x Suitable for digital PCR (dPCR) assays (5x concentration).

MDX074

40 mL

10,000 Rxn

Hi-throughput dUTP qPCR Mix Optimized formulation for efficient dUTP incorporation to remove background PCR product contamination. Inhibitor-Tolerant qPCR Mix Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant qPCR Mix, 5x Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants (5x concentration).

5 mL

500 Rxn

MDX031

100 mL 10,000 Rxn

5 mL

500 Rxn

MDX013

100 mL 10,000 Rxn

2 mL

500 Rxn

MDX073

40 mL

10,000 Rxn

RT-qPCR (RNA Template) Hot-Start Master Mixes

10 mL 1,000 Rxn 100 mL 10,000 Rxn

Fast 1-Step RT-qPCR Mix RT-qPCR mix formulated for fast, automated, high-throughput systems.

MDX032

Inhibitor-Tolerant RT-qPCR Mix, 4x All-in-one, 4x master mix designed for RT-qPCR from crude samples such as sputum, stool and saliva. No sample extraction required. Low LOD 1-Step RT-qPCR Mix Suitable for detecting RNA and DNA viruses at very low levels for applications such as blood bank or transplant viral testing.

1 mL

200 Rxn

MDX016

50 mL 10,000 Rxn

10 mL

1,000 Rxn

MDX025

100 mL 10,000 Rxn

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Optimized Mixes

PRODUCT

CAT NO.

VOLUME

REACTIONS

Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes

5 mL 1,000 Rxn 50 mL 10,000 Rxn

Air-Dryable ™ qPCR Mix, 4x Ready-to-use qPCR mix ideal for oven or air-drying technologies.

MDX082

5 mL

1,000 Rxn

Air-Dryable ™ RT-qPCR Mix, 4x Ready-to-use RT-qPCR Mix ideal for oven or air drying technologies.

MDX095

50 mL

10,000 Rxn

Air-Dryable ™ Direct DNA qPCR Blood, 4x Inhibitor-tolerant qPCR mix ideal for oven or air-drying technologies. Can be used for direct detection using whole blood, serum or plasma samples. Lyo-Ready ™ qPCR Mix Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ qPCR Mix, 2.6x Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets (2.6x concentration). Lyo-Ready ™ 1-Step RT-qPCR Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ 1-Step RT-qPCR Virus Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients and highly suited for amplification of RNA viruses with a high secondary structure. Loop-Mediated Isothermal Amplification (LAMP) Master Mixes Lyo-Ready ™ LAMP Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ RT-LAMP 1-Step Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.

5 mL

1,000 Rxn

MDX092

50 mL

10,000 Rxn

5 mL

500 Rxn

MDX021

100 mL

10,000 Rxn

8 mL

1,000 Rxn

MDX023

100 mL 12,500 Rxn

10 mL

1,000 Rxn

MDX024

100 mL

10,000 Rxn

10 mL

1,000 Rxn

MDX062

100 mL

10,000 Rxn

5 mL

800 Rxn

MDX097

50 mL

8,000 Rxn

5 mL

800 Rxn

MDX108

50 mL

8,000 Rxn

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Polymerases

PRODUCT

CAT NO.

VOLUME

REACTIONS

DNA Polymerases

100 µL 500 Units 10 mL 50,000 Units 10 mL 50,000 Units*

Taq DNA Polymerase DNA Polymerase provided with optimized buffer system for fast PCR reactions across a range of templates. (*Enzyme only)

MDX001

100 µL

500 Units

Taq HS DNA Polymerase Antibody-mediated hot-start enzyme ideal for fast multiplex reactions. Robust performance with low-copy number targets even in the presence of PCR inhibitors. (*Enzyme only) Glycerol-Free Taq HS 50 U/µL Lyophilization-compatible, high concentration (50 U/µL), glycerol free DNA enzyme for automated high-throughput testing (provided as separate antibody, enzyme and dilution buffer). Aptamer Taq HS (Glycerol-Free) 50 U/μL A high concentration, lyophilization-compatible Taq DNA polymerase containing a DNA aptamer which binds reversibly to the polymerase. Suitable for developing highly specific, high-throughput assays.

MDX008

10 mL

50,000 Units

10 mL

50,000 Units*

20 µL 1,000 Units

MDX011

500 µL 25,000 Units

20 µL 1,000 Units

MDX015

500 µL

50,000 Units

Low DNA Taq HS 5 U/µL Heat-activated, thermostable DNA polymerase suited amplification of bacterial and fungal DNA. Low DNA Taq HS 10 U/µL Heat-activated, thermostable DNA polymerase suited to amplification of bacterial and fungal DNA.

100 µL

500 Units

MDX009

10 mL

50,000 Units

50 µL

500 Units

MDX010

5 mL

50,000 Units

High-Fidelity Polymerases High-Fidelity Pfu 3’ - 5’ proofreading exonuclease activity with an error rate of 3.0 x 10 -6 and generates blunt-ended amplicons up to 5 kb in length.

200 µL

500 Units

MDX003

10 mL

25,000 Units

5 mL

200 Rxn

High-Specificity Pfu HS Mix High-fidelity, aptamer-based hot start DNA polymerase tailored for low GC bias amplification.

MDX006

100 mL

4,000 Rxn

Isothermal Polymerases Bst DNA Polymerase

1 mL

Stable, heat resistant DNA polymerase optimized for LAMP and contains 5´ > 3´ DNA polymerase activity and strong strand displacement activity.

MDX012

20 mL

8 µL

High Conc. Glycerol-Free Bst Lyophilization-compatible DNA polymerase for isothermal applications.

MDX018

80 µL

Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media.

2 mL

MDX019

100 mL

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Polymerases

PRODUCT

CAT NO.

VOLUME

REACTIONS

Reverse Transcriptase

8 µL 1,000 Rxn 80 µL 10,000 Rxn

Lyo-compatible MMLV-RT High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays.

MDX042

200 µL 1,000 Rxn

RNase-Tolerant MMLV-RT Mixture of MMLV-RT and RNase inhibitor ideal for RT-qPCR assays.

MDX043

2 mL

10,000 Rxn

200 µL 1,000 Rxn 2 mL 10,000 Rxn

MMLV-RT MMLV-RT suitable for generating first strand cDNA of up to 9 kb.

MDX044

Next-Generation Sequencing NGS Clean and Select Beads Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries. NGS ER Enzyme Mix A mix that processes both 3’ and 5’ overhangs generating product that is 5’ phosphorylated with 3’ A overhang.

50 mL

MDX041

500 mL

MDX040

6 mL

1,000 Rxn

NGS Ligase Enzyme that catalyzes the ligation of adaptors to 3’ A overhangs.

MDX037

2 mL

1,000 Rxn

NGS Library Quantification qPCR-based assay for the quantification of only adapter-ligated library molecules.

MDX039

1 Kit

500 Rxn

Components

PRODUCT

CAT NO.

VOLUME

REACTIONS

Buffers NGS High-Fidelity Pfu Buffer, 10x Optimized for use with High-Fidelity Pfu (MDX003) . NGS End-Repair Buffer, 5x Optimized for use with NGS ER Enzyme Mix (MDX040) .

MDX038

5 mL

1,000 Rxn

MDX035

10 mL

1,000 Rxn

NGS Ligase Buffer, 5x Optimized for use with NGS Ligase (MDX037) .

MDX036

3 mL

1,000 Rxn

Taq PCR Buffer, 5x Optimized for use with Taq DNA Polymerase (MDX001) and Taq HS DNA Polymerase (MDX008) . Bst Reaction Buffer, 10x Optimized for use with Bst DNA Polymerase (MDX012) and High Conc. Glycerol-Free Bst (MDX018) .

MDX002

2 mL

500 Rxn

5 mL

MDX076

10 mL

5 mL

Enzyme Dilution Buffer, 1x A glycerol containing 1x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX078

100 mL

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Components

PRODUCT

CAT NO.

VOLUME

REACTIONS

5 mL

Enzyme Dilution Buffer (10x) Glycerol free A glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX080

10 mL

Tissue Extract-PCR Buffers Lysis and neutralization buffer optimized for use with Taq HS DNA Polymerase (MDX008) to perform PCR direct from crude lysate.

MDX004

1 Kit

1,000 Rxn

2.5 mL 500 Rxn 50 mL 10,000 Rxn

Fast qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) .

MDX033

1-Step RT-qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) and RNase-Tolerant MMLV-RT (MDX043) . Lyo-Ready ™ qPCR Buffer, 2.5x Optimized for use with Glycerol-Free Taq HS (MDX011) . In order to produce lyophilized, ambient- temperature stable qPCR reagents, dNTPs, MgCl 2 and glycerol-free Taq DNA polymerase are not included in the buffer.

2.5 mL

500 Rxn

MDX034

50 mL 10,000 Rxn

8 mL

1,000 Rxn

MDX022

100 mL 12,500 Rxn

2.5 mL 500 Rxn 100 mL 20,000 Rxn

Lyo-Ready ™ qPCR Buffer w/o Excipients, 4x Optimized for use with Glycerol-Free Taq HS (MDX011) .

MDX061

Taq Dilution Buffer This Buffer provides the optimal conditions to store Meridian polymerases, conferring long-term stability at -20 °C (MDX008) . Inhibitor-Tolerant PCR Buffer, 5x Used with Taq HS DNA Polymerase (MDX008) for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media. General Reagents Uracil DNA Glycosylase (UDG) # Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free. Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA.

5 mL

MDX007

100 mL

2 mL

500 Rxn

MDX075

100 mL 20,000 Rxn

2 mL

MDX019

100 mL

MDX054

10 mL

10,000 Units

MDX055

25 mL

500 mg

RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays. Taq HS Antibody A mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity at room temperature. For use in hot-start PCR.

250 µL 10,000 Units

MDX056

2.5 mL 100,000 Units

20 µL 1,000 Units

MDX014

250 µL 12,500 Units

# Not available in the US

* Not available in China

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Ready-to-use sequencing grade dNTPs available individually, as sets, or pre-blended dNTP mixes. Ultra-pure (>99% by HPLC) free of PCR inhibitors, DNase and RNase.

dNTPs

PRODUCT

CAT NO.

VOLUME

dNTPs Lithium Salt dNTP Set, 100 mM Provided as four separate solutions at 100 mM concentration (dATP, dCTP, dGTP & dTTP).

4 x 250 µL 4 x 50 mL

MDX050

500 µL 50 mL 100 mL 1 L

dNTP Mix, 100 mM Supplied as a mixture (dATP, dCTP, dGTP & dTTP). Total concentration of mix is 100 mM (25 mM of each nucleotide).

MDX051

dUTP Mix, 50 mM Supplied as a mixture (10 mM dATP, dCTP, dGTP and 20 mM dUTP).

MDX058

50 mL

dUTP, 100 mM

MDX059

50 mL

dATP, 100 mM

MDX046

50 mL

dCTP, 100 mM

MDX047

50 mL

dGTP, 100 mM

MDX048

50 mL

dTTP, 100 mM

MDX049

50 mL

dNTPs Sodium Salt dNTP Mix, 10 mM sodium salt*

MDX067

Please Enquire

dNTP Mix, 40 mM sodium salt*

MDX083

Please Enquire

dNTP Mix, 100 mM sodium salt*

MDX084

Please Enquire

dATP Mix, 100 mM sodium salt*

MDX063

Please Enquire

dCTP Mix, 100 mM sodium salt*

MDX064

Please Enquire

dGTP Mix, 100 mM sodium salt*

MDX065

Please Enquire

dTTP Mix, 100 mM sodium salt*

MDX066

Please Enquire

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Designed for validating the steps within a qPCR or RT-qPCR assay to produce more reliable and accurate results.

qPCR & RT-qPCR Extraction Controls

The qPCR Extraction Controls are E. coli cells of a known concentration containing the Internal Control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample DNA). Genetic material from the test sample and the DNA extraction control are simultaneously extracted by common extraction methods with the control being as sensitive to the same inhibition and extraction failure as the test sample. qPCR

Product Highlights

• E xtraction control undergoes the same sample processing as test sample • C an be used with a variety of sample types including inhibitor-rich samples such as blood, urine and sputum

• C onfirms successes of extraction step and monitors co-purification of PCR inhibitor inhibitors • Probe based designed specifically for multiplex qPCR assays • Control DNA is unique with no homology to any organism

PRODUCT

CAT NO. VOLUME REACTIONS

qPCR Extraction Control RED (Quasar ® 670)

MDX026

10 mL 2,000 Rxn 10 mL 2,000 Rxn

qPCR Extraction Control ORANGE (Cal Fluor ® 560) MDX027

VLP-RNA Extraction Control is RNA molecule without any known homology, protected in a protein nanocage, harnessing the stability of viral structures. VLP-RNA Extraction Control is spiked into the biological sample and undergoes the entirety of the sample processing, enabling users of RT-qPCR assay to not only validate the success of the extraction and the reverse transcription steps, but also to determine the presence of PCR inhibitors, reducing the chance of obtaining false negatives and resulting in reliable and accurate results. The VLP-RNA Extraction Control is provided with its own detection system and can have its sequence customized if necessary. RT-qPCR

Product Highlights

• C ontains a defined number of copies of target RNA molecules, encapsidated within a virus-like particle (VLP) • R NA sequence is customizable up to 1000nt • N on-infectious material for ease of handling and shipping

• C losely mimics the test sample, undergoing the same processing route from lysis and extraction to RT-qPCR detection • C ompatible with commonly used RNA extraction methods and lyophilization

PRODUCT

CAT NO.

REACTIONS

1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL) 1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL)

VLP-RNA Extraction Control Red

MDX068

VLP-RNA Extraction Control Orange

MDX069

VLP-RNA Extraction Control

MDX071

Please enquire

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Antigens & Antibodies for Immunoassays

DESCRIPTION TYPE

APPLICATION CAT #

DESCRIPTION TYPE

APPLICATION CAT #

Brucella abortus Intact Cells

African Swine Fever Virus (ASFV) p30 Protein MAb

C86116M

ELISA, IFA, IHC, IP, WB C01881M ELISA, IFA, IHC, IP, WB C01882M

MAb MAb

ELISA, WB ELISA, WB

C86131M

Intact Cells

p30 Protein p30 Protein p30 Protein p62 Protein p30 Protein p30 Protein p54 Protein

MAb MAb MAb

9609

Coronavirus, Bovine Surface Antigen (Peplomer)

ELISA, LF, Pair ELISA, LF, Pair

9610

C86540M

MAb

ELISA, HIA

9605

Ag (Rec.) Ag (Rec.) Ag (Rec.) Ag (Rec.)

ELISA, WB

Cryptosporisium , Bovine Intact Oocysts

R01793

ELISA ELISA ELISA

B65651G

PAb (Goat) ELISA, IFA

R01795

Foot-and-mouth disease (FMD) Non-Structural Protein (NS) MAb

R01796

Nipah Virus (NiV) G Protein

C01452M

ELISA

C01974M

MAb MAb

ELISA, pair ELISA, pair

C01448M

Serotype O1

MAb

ELISA

C01975M

G Protein

Nebraska Calf Diarrhea Virus (NCDV, Rotavirus) NCDV p43 (vp6) MAb ELISA

G Protein (Malaysia strain)

R01766

Ag (Rec.)

ELISA, pair

C11222M

B65110G

NCDV (ICPs) NCDV (ICPs) NCDV (ICPs) NCDV (ICPs)

PAb (Goat) IFA PAb (Goat) ICC PAb (Goat) IFA PAb (Goat) IFA

Porcine Epidemic Diarrhea Virus (PEDV) PEDV MAb

B65213G

C02005M

ELISA, LF, WB, pair ELISA, LF, WB, pair

B65211G

C02006M

PEDV

MAb

B65212G

R01778

Nucleoprotein

Ag (Rec.)

ELISA, LF, WB

Low Density Lipoprotein (LDL) Ligand binding region within repeat #1 MAb Luteinizing Hormone (LH) LH (X-reacts with sheep, goat and deer) PAb (Rabbit)

Transmissible Gastroenteritis Virus (TGV) Peplomer Protein MAb ELISA, Neut

C55802M

H44070M

IFA, WB, FC

C55803M

Peplomer Protein MAb

ELISA

D01237R

ELISA, IHC, WB

Trichomonas foetus T. foetus

C01573M

MAb MAb

ELISA, IFA, pair ELISA, IFA, pair

C01572M

T. foetus

Bovine Viral Diarrhoea Virus BVDV-Erns EP Glycoprotein Ag (Rec.)

R01801

ELISA, LF, WB

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Antigens & Antibodies for Immunoassays

Description

Type

Application

Cat #

Description

Type

Application

CAT #

Parvovirus Parvovirus Parvovirus Parvovirus Parvovirus Parvovirus

C02003M

MAb MAb MAb MAb

ELISA, LF ELISA, LF

Canine Distemper Virus (CDV) Surface Envelope Antigen MAb

C02004M

C86504M

ELISA, LF, IFA, pair

C86004M

ELISA, LF, pair

C86005M

ELISA, pair

Surface Envelope Antigen

C86801M

MAb

ELISA, IFA, pair

A01727C

Ag (Native) ELISA

C01986M

CDV CDV CDV CDV CDV CDV

MAb MAb MAb MAb MAb

ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair

VP2 Protein R01770 pro-Brain Natriuretic Peptide, N-Terminal (NT-proBNP) N-Terminal, a.a. 40-50 MAb ELISA H01353M N-Terminal, a.a. 64-72 MAb ELISA H01354M Serum Amyloid A (SAA) SAA MAb ELISA, WB, pair H01381M SAA MAb ELISA, WB, pair H01383M SAA MAb ELISA, WB, pair H01384M SAA MAb ELISA, WB, pair H01382M SAA MAb ELISA, WB, pair H01425M SAA MAb ELISA, WB, pair H01426M SAA MAb ELISA, WB, pair H01427M SAA MAb ELISA, WB, pair H01429M SAA MAb ELISA, WB, pair H01430M SAA Ag (Rec.) ELISA, pair R01771 Thyroid Stimulating Hormone (TSH) Beta subunit MAb ELISA, WB, pair E01369M Beta subunit MAb ELISA, WB, pair E01370M Thyroglobulin Thyroglobulin Ag (Native) ELISA A01725C Ag (Rec.) ELISA, pair

C01987M

C01988M

C01989M

C01990M

A01726C

Ag (Native) ELISA

Coronavirus Coronavirus Coronavirus Coronavirus Nucleoprotein Nucleoprotein

C02011G

Goat MAb MAb MAb MAb MAb

ELISA, IEP, pair

C01998M

ELISA, pair ELISA, pair ELISA, pair ELISA, pair

C01999M

C02000M

C02009M

C02010M

Nucleocapsid

ELISA, LF, WB, pair

A01730N

Coronavirus

Ag (Native) ELISA

C-Reactive Protein CRP

M01345M

MAb MAb MAb MAb

ELISA, pair ELISA, WB ELISA, pair ELISA, pair ELISA, pair

M01346M

CRP CRP CRP CRP

M01347M

M01348M

R01772

Ag (Rec.)

Cystatin C Cystatin C

H01303M

MAb MAb

ELISA, pair ELISA, pair

H01304M

Cystatin C

Heartworm (Dirofilaria immitis) D. immitis MAb

C01991M

ELISA, pair ELISA, pair ELISA, pair

C01992M

D. immitis D. immitis

MAb MAb

C01993M

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Antigens & Antibodies for Immunoassays

Description

Type

Application

Cat #

Description

Type

Application

CAT #

Cystatin C Cystatin C Cystatin C Cystatin C

Infectious Bronchitis Virus (IBV) Nucleoprotein MAb Infectious Bursal Disease (IBDV) VP2 Protein MAb

H01431M

MAb MAb MAb

ELISA, pair ELISA, pair ELISA, pair

C01530M

ELISA, WB

H01303M

H86217M

C01524M

ELISA, WB, pair ELISA, WB, pair

Feline Immunodeficiency Virus (FIV) Core Antigen Ag (Rec.)

C01526M

VP2 Protein VP3 Protein VP3 Protein

MAb MAb MAb

R65360

ELISA, LF

ELISA, WB, IHC, pair C01525M ELISA, WB, IHC, pair C01528M

A01731F

FIV

ELISA

Feline Leukemia Virus (FeLV) Glycoprotein 70 (gp70) MAb

Influenza A H5 (H1H5) Hemagglutinin H5 MAb Hemagglutinin H5 MAb Influenza A H7 (H1H7) Hemagglutinin H7 MAb Hemagglutinin H7 MAb Marek Disease Virus Serotypes 1, 2 & 3 MAb Serotypes 1, 2 & 3 MAb Serotypes 1, 2 & 3 MAb

ELISA, DB, HIA, pair C86240M

C65712M

ELISA, IFA

C01309M

ELISA, pair

C01995M

p27 Protein p27 Protein p27 Protein p27 Protein

MAb MAb

ELISA, WB, pair ELISA, WB, pair

C01996M

C01610M

ELISA, WB ELISA, WB

B65221G

PAb (Goat) WB

C01611M

C01997G

PAb (Goat) ELISA, IFA, WB

p27 Protein (a.a. 272 - 519)

C01264M

ELISA ELISA ELISA

R01776

Ag (Rec.)

ELISA, WB

C01267M

Parvovirus

C01268M

Ag (Lysate)

A01728F

Parvovirus

ELISA

Newcastle Disease Virus (NVD) HN Glycoprotein MAb

C86012M

ELISA, HIA ELISA, HIA

Serum Amyloid A (SAA) SAA MAb

C86014M

HN Glycoprotein MAb HN Glycoprotein MAb HN Glycoprotein MAb HN Glycoprotein MAb Ribonucleoprotein MAb

H01381M

ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair

C86016M

ELISA, HIA, WB

H01383M

SAA SAA SAA SAA SAA

MAb MAb MAb MAb

C01351M

ELISA, HIA

H01425M

C01352M

ELISA

H01426M

C01629M

ELISA, IFA, IHC

H01427M

R01774

Ag (Rec.)

ELISA

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Antigens & Antibodies for Immunoassays

Description

Type

Application

Cat #

Description

Type

Application

CAT #

Suitable for use with dog, bovine, monkey and rat serum

Burkholderia (Pseudomonas) mallei Whole cells MAb

Adenovirus Hexon Hexon Antigen MAb

C01752M

Indirect ELISA

ELISA, IHC, ID, pair C86804M

C86315M

LPS

MAb

ELISA, WB

Hexon Antigen of 1, 5, 8 & 27 Hexon Antigen of 1, 5, 8 & 27

MAb, biclonal MAb, biclonal

Cystatin C Cystatin C Cystatin C Cystatin C

C86006M

ELISA, LF, pair

H01303M

MAb MAb MAb

ELISA, pair ELISA, pair ELISA, pair

C86007M

ELISA, ID, pair

H86013M

H01431M

Borrelia (Lyme Disease) B. burgdorferi garinii MAb

Serum Amyloid A (SAA) SAA MAb

C86420M

ELISA, IFA, WB

H01381M

ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair ELISA, WB, pair

H01383M

SAA SAA SAA SAA SAA SAA SAA SAA SAA

MAb MAb MAb MAb MAb MAb MAb MAb

ELISA, LF, CLIA, WB R01609

B. afzelii VlsE

Ag (Rec.)

H01384M

B. burgdorferi VlsE, multi-epitope chimera B. garinii p14 antigen (flagellin)

R01523

Ag (Rec.)

ELISA, DB, WB

H01382M

H01425M

ELISA, LF, CLIA, BD, WB

R01521

Ag (Rec.)

H01426M

H01427M

ELISA, LF, CLIA, WB R01610

B. garinii VlsE

Ag (Rec.)

H01429M

Cryptosporidium parvum C. parvum MAb

H01430M

C02007M

ELISA, LF, WB, pair ELISA, LF, WB, pair

R01773

Ag (Rec.)

ELISA, pair

C02008M

C. parvum

MAb

Rabies

Virus Neut., ELISA, IHC, IFA

C86307M

Rabies

MAb

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Recommended Blockers

Blockers for interference in sandwich immunoassays

Animal IgG – Passive Blockers • Suited for mixed species assays (e.g. MAb/PAb) • Species of blocker must be the same as the host of the capture or detection antibody

Passive blocking reagents work by preventing interfering antibodies from binding to the capture or detection antibodies by providing alternate binding sites. Animal IgG (e.g. Goat IgG) can only block one type of interference (e.g. human anti-goat antibodies) so typically more than one type must be used, depending on the host of both the capture and detection antibodies. Animal IgG must be added in excess concentration and the effectiveness depends on the affinity of interfering antibody for the animal IgG. RECOMMENDED CONCENTRATION: • 10x the concentration of the MAb/PAb being used in the assay (e.g. if 5μg/mL of Ab/conjugate, add 50μg/mL Animal IgG). • Can be added to the sample or conjugate diluent but ideally should be in contact with the patient sample before incubation with the assay capture antibody. In double mouse monoclonal assays, a specific blocker is required to remove a particular type of HA interference called human anti-mouse antibodies (HAMA) and Rheumatoid Factor (RF). A HAMA blocker contains a specific binder directed against all types of heterophilic interference including HAMA and RF. Once bound to the interfering antibodies, TRU Block prevents further binding of HA to other assay components through steric hindrance. Active blockers can typically be used in lower concentrations than passive blocking reagents, which minimizes the reduction in assay signal commonly associated with passive blockers. RECOMMENDED CONCENTRATION: For best performance, TRU Block should be included as part of the sample or conjugate diluent, at a recommended concentration range: Product Protein Concentration Application TRU Block ™ Ready Single-step dilution with recommended dilution of 1:1000 to 1:10 ELISA & LF

Mouse IgG A66186M (9-13mg/mL) A66185M (50-55mg/mL) A66189M (45-55mg/mL, No Azide) A66185M-LY (Lyophilized)

Goat IgG A66200H

Rat IgG A64391R

Chicken IgY A01302C (Lyophilized)

Rabbit IgG A66100H

Sheep IgG A66400S (70-77mg/mL, Liquid)

TRU Block ™ – Active HAMA & RF Blocker • Suited for double mouse monoclonal assays • Removes HAMA, HA & RF interference

TRU Block ™ Ready 8001 TRU Block ™ Ultra 8000 TRU Block ™ A66800H TRU Block ™ 2 A66802H TRU Block ™ 3 A66803H

Interfering HA (e.g. HAMA) TRU Block

TRU Block

IgM RF

Detection Antibody (mouse origin) Capture Antibody (mouse origin) Antigen

Blocking Buffer

*Formulations differ in their ratio of various proprietary ingredients that confers unique blocking characteristics.

TRU Block ™ ULTRA TRU Block ™ TRU Block ™ 2 TRU Block ™ 3

Range: 24-26 mg/mL ELISA, CLIA & LF

Range: 24-26 mg/mL ELISA & CLIA

Range: 24-26 mg/mL

CLIA & LF

24.3 mg/mL

ELISA

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Pairs

Capture Antibody

Detection Antibody

Capture Antibody

Detection Antibody

Antigen Application

C86804M C86006M C86007M C86006M

Infectious Bursal Disease (IBDV)

C01524M C01526M

Adenovirus

C01525M C01528M

C. parvum

C02008M C02007M

Nipah Virus C01975M C01974M R01766

C01986M C01988M C01987M C01988M C01990M C01989M C86504M C86801M C01998M C01998M C02010M C02009M C02009M C20211G C02010M C20211G C01999M C01998M

C02003M C02004M C86005M C86004M R01770

Parvovirus

Canine Distemper Virus

Porcine Epidemic Diarrhea Virus (PEDV)

C02006M C02005M R01778

H01381M H01383M H01426M H01425M R01771 H01426M H01425M R01773 H01426M H01425M R01773 H01427M H01425M R01771 H01427M H01425M R01773 H01427M H01425M R01774 H01429M H01384M R01771 H01429M H01384M R01773 H01429M H01383M R01771 H01429M H01383M R01773 H01384M H01430M R01771 H01384M H01430M R01773 H01382M H01384M

Coronavirus

M01347M M01345M R01772 M01348M M01345M R01772

CRP

Serum Amyloid A (SAA)

H01303M H86013M H01304M H01303M H01431M H01303M H01431M H86217M

Cystatin C

Feline Leukemia Virus (FeLV)

C01996M C01995M

H1H5 C01309M C86240M

Trichmonas foetus

C01992M C01992M C01993M C01992M

C01573M C01572M

Heartworm

TSH E01370M E01369M

Canine

Bovine

Equine

Avian

Feline

Swine

Multi-species

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