Designed for validating the steps within a qPCR or RT-qPCR assay to produce more reliable and accurate results.
qPCR & RT-qPCR Extraction Controls
The qPCR Extraction Controls are E. coli cells of a known concentration containing the Internal Control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample DNA). Genetic material from the test sample and the DNA extraction control are simultaneously extracted by common extraction methods with the control being as sensitive to the same inhibition and extraction failure as the test sample. qPCR
Product Highlights
• E xtraction control undergoes the same sample processing as test sample • C an be used with a variety of sample types including inhibitor-rich samples such as blood, urine and sputum
• C onfirms successes of extraction step and monitors co-purification of PCR inhibitor inhibitors • Probe based designed specifically for multiplex qPCR assays • Control DNA is unique with no homology to any organism
PRODUCT
CAT NO. VOLUME REACTIONS
qPCR Extraction Control RED (Quasar ® 670)
MDX026
10 mL 2,000 Rxn 10 mL 2,000 Rxn
qPCR Extraction Control ORANGE (Cal Fluor ® 560) MDX027
VLP-RNA Extraction Control is RNA molecule without any known homology, protected in a protein nanocage, harnessing the stability of viral structures. VLP-RNA Extraction Control is spiked into the biological sample and undergoes the entirety of the sample processing, enabling users of RT-qPCR assay to not only validate the success of the extraction and the reverse transcription steps, but also to determine the presence of PCR inhibitors, reducing the chance of obtaining false negatives and resulting in reliable and accurate results. The VLP-RNA Extraction Control is provided with its own detection system and can have its sequence customized if necessary. RT-qPCR
Product Highlights
• C ontains a defined number of copies of target RNA molecules, encapsidated within a virus-like particle (VLP) • R NA sequence is customizable up to 1000nt • N on-infectious material for ease of handling and shipping
• C losely mimics the test sample, undergoing the same processing route from lysis and extraction to RT-qPCR detection • C ompatible with commonly used RNA extraction methods and lyophilization
PRODUCT
CAT NO.
REACTIONS
1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL) 1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL)
VLP-RNA Extraction Control Red
MDX068
VLP-RNA Extraction Control Orange
MDX069
VLP-RNA Extraction Control
MDX071
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