DNA Polymerases
Standard & Hot-Start Taq DNA Polymerase
Taq DNA polymerase is from the thermophilic bacterium Thermus aquaticus and is a heat-stable enzyme commonly used in PCR. Meridian Bioscience’s Taq DNA polymerases are designed for fast and sensitive PCR from complex templates and are available in several hot-start formats including antibody, chemical and aptamer-based technology. The enzymes are supplied with a novel buffer containing dNTPs, MgCl 2 and enhancers that have been optimized for robust amplification and fast cycling conditions, considerably reducing the reaction time without compromising on PCR sensitivity or yield. Hot-start enzymes also ensure that a reaction remains completely inactive during PCR set-up to prevent non-specific amplification.
SPECIFICITY: Highly sensitive and specific amplification using antibody hot-start Taq DNA polymerase to detect down to 1 copy of template
Using antibody hot-start Taq DNA polymerase (MDX008) in Air-Dryable ™ Direct DNA qPCR Blood mix to detect EGFR Exon 19 mutation from 10% human plasma extract. The data demonstrates the specific and sensitive detection of a single copy of EGFR Exon19 deletion amongst 10,000 EGFR Exon19 wildtype copies.
EGFR_EX19 Deletion | EGFR_EX19 Widetype
THERMOSTABILITY: Stable at room temperature for up to one month, streamlining manufacturing processes and enabling convenient shipping and handling
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4 ℃ | room temperature
The stability of Taq HS DNA Polymerase (MDX008) is examined in Air-Dryable ™ Direct RNA/DNA qPCR Saliva Mix in a triplex reaction on A) g-actin B) b2mg and C) GAPDH DNA targets at two different concentrations, after being stored at 4°C ( blue ) and room temperature ( red ) for one month. The results illustrate that the stability of the Taq HS DNA polymerase is unaffected, with no loss of functional activity, even at room temperature for one month, facilitating manufacturing process and allowing for easy shipping and handling.
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