DNA Polymerases
Glycerol-free Taq DNA Polymerase
Glycerol-free polymerases are ideal for developing ambient-temperature stable assays and offer performance advantages in terms of activity and stability. Meridian’s Glycerol-Free Taq HS polymerase (MDX011) and Glycerol-Free Taq HS High Conc. DNA Polymerase (MDX170) are high-concentration (50 U/ μ L) enzymes optimized to deliver robust amplification without glycerol. The high concentration format of the enzymes is well suited for assay optimization and enzyme titration. In addition, Meridian Glycerol-Free Taq HS DNA Polymerases are compatible with drying technologies such as air-drying or lyophilization. LYO-COMPATIBILTIY: Glycerol-Free Taq Polymerase exhibits the same efficiency and sensitivity after lyophilization
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Glycerol-Free Hot Start Polymerase is compatible with lyophilization without any loss in sensitivity and speed ”
Wet mix | Lyophilized mix
A 10-fold serial dilution of human DNA (10 3 copies down to 10 copies) using Glycerol-Free Taq HS 50U/ μ L (MDX011) as a wet mix ( black ) or lyophilized mix ( blue ) to illustrate that lyophilization does not have an effect on the quality of the Glycerol-Free Taq HS.
Proof-reading Pfu DNA Polymerase
Pfu is a high-fidelity antibody-mediated hot-start DNA polymerase from the hyperthermophilic archea Pyrococcus furiosus (Pfu). The Pfu polymerase enzyme synthetises DNA in the 5´ → 3´ direction and also possesses a 3´ → 5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. Meridian’s High- Fidelity Pfu has an error rate of 3.0 x 10 -6 generating blunt-ended amplicons. It can be supplied in a novel buffer system containing dNTPs and enhancers, along with MgCl 2 and is perfect for applications such as target enrichment, NGS library amplification, or cloning. HIGH-FIDELITY: Proof-reading activity of Pfu allows for accurate NGS library amplification.
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Meridian’s High-Fidelity Pfu exhibits an error rate of 3.0 x 10 -6 ”
Four NGS libraries were created using E. coli DNA. The libraries were amplified using High-Fidelity Pfu (MDX003) prior to being placed on flow cells and sequenced. For each sample, the fraction of uniquely mapped (mapped to only one place on a reference sequence), ambiguously mapped and unmapped reads relative to the total number of reads per sample is shown. The results illustrate that with E. coli DNA, High-Fidelity Pfu allowed for over 90% unique reads that could be aligned to the reference sequence.
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