DNA Polymerases
Thermostable Tth DNA Polymerase
HIGH-THERMOSTABILITY: Enhances sensitivity for targets with complex secondary structures via high-temp amplification Glycerol-Free HS Tth DNA Polymerase (HC) is a high-concentration thermostable DNA polymerase from Thermus thermophilus with high reverse transcriptase activity in the presence of Mn2+ ions. Its high processivity and reverse transcriptase activity at elevated temperatures allow Glycerol-Free HS Tth DNA Polymerase (HC) to overcome the problems posed by RNA secondary structures. Glycerol-Free HS Tth DNA Polymerase uses antibody-mediated hot start technology that enhances the specificity and sensitivity of PCR. It is supplied with a reaction buffer that contains dNTPs and excipients required for lyophilization. Glycerol-Free HS Tth DNA Polymerase (HC)enables flexible and scalable reaction volumes, and is suitable for applications such as fast RT-qPCR amplification of difficult templates from crude samples.
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Meridian’s Tth polymerase enables high-efficiency amplification of structurally complex RNA and DNA templates ”
Performance of MDX205 ( red ), MMLV-RT ( blue ) and AMV-RT ( black ) after pre-incubation from 40°C to 95°C for 10 mins, in a multiplex one-step RT-qPCR assay on mouse RNA. ∆ Ct values were calculated against the Ct value produced by the same enzyme stored at -20 °C. The data illustrates that MDX205 maintains its full performance even after 10 min at 95°C, in contrast to MMLV-RT and AMV-RT which are inactivated above 55°C and 60°C, respectively.
Isothermal Enzymes
Glycerol-free Phi29 DNA Polymerase
Glycerol-Free Phi29 DNA Polymerase (HC) is a highly processive DNA-dependent DNA polymerase with strong strand displacement activity, which enables efficient isothermal DNA amplification from low DNA inputs. Glycerol-Free Phi29 DNA Polymerase (HC) high-fidelity amplification, makes it useful for sequencing DNA template preparation, rolling circle amplification (RCA) and multiple displacement amplification (MDA).
AMPLIFICATION POWER: Strong DNA yield even at low template concentrations
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Glycerol-Free Phi29 is Proven to Deliver Higher Amplification Efficiency ”
DNA polymerase activity of lyophilized Glycerol-Free Phi 29 DNA Polymerase (HC) ( red ) was measured in a rolling circle amplification (RCA) assay, to amplify single stranded circular microphage DNA, and compared against Φ 29 DNA Polymerase (high concentration) from Qiagen ( black ). The results demonstrate that Glycerol-Free Phi 29 DNA Polymerase (HC) has greater DNA polymerization activity than the product from other suppliers.
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