Isothermal Enzymes
Bst DNA Polymerase
Bst DNA Polymerase, short for Bacillus stearothermophilus DNA Polymerase, is a type of enzyme commonly used in isothermal DNA amplification methods such as Loop-Mediated Isothermal Amplification (LAMP), Isothermal Helicase-Dependent Amplification (HAD) and Isothermal Multiple Cross Displacement Amplification (MCA). Meridian’s Bst DNA Polymerase exhibits fast 5´- 3´ DNA polymerase activity and strong strand displacement activity to deliver fast amplification speed (time-to-results), yield, salt tolerance, and sensitivity.
SPEED: Bst DNA Polymerase exhibits superior speed and specificity
COMPATIBILTIY: Glycerol-Free Bst Polymerase demonstrates faster time-to-results (TTR) and better reproducibly compared to glycerol containing enzymes
Meridian Bst Polymerase | NEB Bst 2.0 | NEB Bst
Meridian Bst Polymerase | NEB Bst 2.0 | NEB Bst | ArcticZymes
A human pathogenic parasite (Strongyloides stercoralis) was screened using Bst Polymerase ( red ), NEB Bst 2.0 ( purple ) and NEB wild-type Bst Large Fragment ( green ), using the same test conditions across four technical replicates..
CFTR (cystic fibrosis transmembrane receptor) was screened using High Conc. Glycerol-Free Bst ( red ), NEB Bst 2.0 ( purple ), NEB wild-type Bst Large Fragment ( green ) and ArcticZymes IsoPol DNA Polymerase ( blue ), using the same test conditions across four technical replicates..
Nucleic Acid Sequence-Based Amplification (NASBA) is an isothermal amplification technique that uses three enzymes (reverse transcriptase, RNase H, and T7 RNA polymerase) to directly amplify an RNA target sequence at 41°C . Meridian offers a full solution for NASBA enzymes, empowering fast and sensitive isothermal amplification direct from an RNA target. These enzymes are glycerol- free and compatible with lyophilization to develop ambient temperature stable assays, ideal for point-of-care testing. NASBA Enzymes SPEED: High specificity of detection with Meridian’s NASBA enzymes under 1 hour with no detectable NTCs SENSITIVITY: Sensitive detection with Meridian’s NASBA enzymes down to 10 copies of RNA under 1 hour
HIV-1 RNA
0 10 20 30 40
1000 copies 100 copies 1000 copies
100 copies 10 copies
10 copies
HIV-1 RNA | NTC
HIV-1 RNA
Primers and a Molecular Beacon probe were designed for HIV-1 RNA and used in NASBA reactions, with a 1,000 copy, 100 copy and 10 copy serial dilution of the RNA targets.
Primers and a Molecular Beacon probe were designed for HIV-1 RNA and used in a nucleic acid sequence-based amplification (NASBA) reaction using Glycerol-Free T7 RNA Polymerase (HC) (MDX201), Glycerol-Free RNase H (HC) (MDX202) and AMV reverse transcriptase, using 1,000 copies of an HIV-1 RNA target.
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