Meridian High-performing Enzymes Guide 0525

Reverse Transcriptases

MMLV-RT

Reverse transcriptases are essential for synthesizing complementary DNA (cDNA) strands from RNA templates. They direct the synthesis of first strand cDNA, which can be used directly as a template for applications such as PCR, qPCR, LAMP, or sequencing workflows. Meridian offers several RTs, each containing unique product features such as high thermostability or lyophilization compatibility for creating room-temperature stable mixes.

One Step RT-qPCR/ RT-LAMP

Two Step RT-qPCR/ RT-LAMP

View our selection chart for details:

Thermostability

Name / Features

Conc.

Cat. No

55C MMLV-RT Compatible with lyophilization or air-drying

MDX117

200 U/ µ L

Up to 60°C

RNase-Tolerant MMLV-RT Contains an RNase inhibitor to improve reaction efficiency

MDX043

Up to 50°C

100x

MMLV-RT Lower RNase H activity

MDX044

100x

Up to 45°C

Lyo-Compatible MMLV-RT Glycerol-free formulation

MDX042

100x

THERMOSTABILITY: Meridian’s 55C MMLV-RT exhibits better thermostablility at temperatures up to 60°C

SENSITIVITY: Meridian’s Lyo-Compatible MMLV-RT allows high sensitivity of low copy number RNA targets

55C MMLV-RT | MMLV-RT | SuperScript III Reverse Transcriptase

Hepatitis virus RNA | NTC

Performance of Meridian’s 55C MMLV-RT ( red , MDX117), standard MMLV-RT ( grey , MDX044) and a SuperScript III Reverse Transcriptase (Thermo, black ) after pre-incubation at 40°C to 70°C for 10 mins, in a multiplex one-step RT-qPCR assay. Δ ct values were calculated against the ct value produced by the same enzyme stored at -20 °C. The data illustrates the increased thermal stability of 55C MMLV-RT when compared to other MMLV-RT enzymes and its ability to efficiently synthesize cDNA at temperatures up to 60°C.

The high efficiency of Lyo-compatible MMLV-RT (MDX042) when used with Lyo-Ready qPCR Mix (MDX021) to detect Hepatitis virus (RNA) allows for high sensitivity detection of low copy number targets (as few as five viral particles).