Next Generation Sequencing (NGS)
NGS is becoming increasingly accessible, affordable, and user-friendly across research, clinical, and industrial settings. However, most NGS workflows still rely on cold-chain shipping and storage for assay reagents. Meridian’s glycerol-free NGS enzymes support lyophilization, offering a sustainable, ambient-stable alternative. Supplied at high concentration, these enzymes are also ideal for assay miniaturization — a key requirement for point-of-care devices.
Glycerol-Free T4 DNA Polymerase (HC)
A template dependent DNA polymerase that catalyzes the addition of nucleotides to the 3’ end of a DNA strand, extending it in the 5’ to 3’ direction. It also possesses 3’ to 5’ exonuclease activity but lacks a 5´to 3´ exonuclease activity. In NGS library preparation it can be used separately or as part of an End-Repair Mix, where it can blunt DNA ends of double-stranded DNA fragments for subsequent ligation of adaptors.
No loss of activity after lyophilization
Lyophilized Glycerol-Free T4 DNA Polymerase (HC) ( red ) was incubated at 37°C for 1 month and its DNA polymerization activity tested against a non-lyophilized aliquot stored at -20°C ( blue ). The results show that the lyophilized Glycerol-Free T4 DNA Polymerase (HC) is stability at ambient temperature for 12 months.
Glycerol-Free DNA Pol I Klenow Fragment (HC)
A large N-terminal truncated protein fragment produced from E. coli DNA polymerase I, which retains polymerase and 3’-5’ exonuclease activity but has lost 5’-3’ exonuclease activity. It can be used to fill in 5´-protruding ends and is ideal for DNA blunting by 3’ overhang removal and fill-in of 5’ overhangs prior to adapter ligation in next-generation sequencing library preparation.
Fast kinetics with high performance
Glycerol-Free DNA Pol I Klenow Fragment (HC) was used in a primer extension assay and compared to other suppliers under the same conditions. The results demonstrate that Glycerol-Free DNA Pol I Klenow Fragment (HC) has a faster reaction rate and higher end fluorescence than most suppliers.
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