Detection of Cervical Cancer-associated p53 Mutation from 20% Pooled Human Urine
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B)
Detection of cervical cancer-associated p53 mutation (SNP accession number: rs1042522) was performed using A/ Lyo-Ready ™ Genotyping Direct qPCR Urine, B/ Kapa Probe Force, C/ TaqPath ™ and D/ Type-it Fast Kits, in presence of 20% pooled human urine. Homozygous Allele G ( blue ) and Allele C ( red ) and heterozygous Allele C/G ( green ) with a NTC ( black ) and x for undetermined. Meta-analysis suggested that p53 rs1042522 C>G polymorphisms are associated with the increased risk of cervical cancer 3 . Genotyping analysis from the Lyo-Ready ™ Genotyping Direct qPCR Urine Mix illustrate accurate allelic discrimination clustering in the presence of 20% urine.
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D)
Allele G | Allele C | Allele C/G | NTC
Detection of Bladder Cancer-associated PAI1 Mutation from 30% Pooled Human Urine
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B)
Detection of bladder cancer-associated PAI1 mutation (SNP accession number: rs7242) was performed using A/ Lyo-Ready ™ Genotyping Direct qPCR Urine, B/ Kapa Probe Force, C/ TaqPath ™ and D/ Type-it Fast Kits, in presence of 30% pooled human urine. Homozygous Allele G ( blue ) and Allele T ( red ) and heterozygous Allele G/T ( green ) with a NTC ( black ) and x for undetermined. Bladder cancer is one of the most common cancer types worldwide and is characterized by a high rate of recurrence, recent studies suggest SNP rs7242 increased the anti-apoptotic effect of plasminogen activator inhibitor-1 (PAI1) indicating a potentially higher risk of bladder cancer recurrence 4 . Genotyping analysis from the Lyo-Ready ™ Genotyping Direct qPCR Urine Mix illustrate accurate allelic discrimination clustering in the presence of 30% urine.
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D)
Allele G | Allele T | Allele G/T | NTC
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