PCR & qPCR Applications
Taq DNA Polymerase & Taq Hot-Start (HS) DNA Polymerase
Taq DNA polymerase is from the bacterium Thermus aquaticus and is a heat-stable enzyme commonly used in PCR. Meridian’s Taq DNA polymerase is designed for fast and sensitive PCR from complex templates and is supplied with a novel buffer containing dNTPs, MgCl 2 and enhancers that have been optimized for robust inhibitor tolerance and fast cycling conditions, considerably reducing the reaction time without compromising on PCR sensitivity or yield. It is also available as an antibody-mediated hot start enzyme, ensuring a reaction remains completely inactive during PCR set-up to prevent non-specific amplification.
> Fast DNA polymerase, delivering high yield under very fast PCR cycling conditions (less than 30 min) > Novel optimized buffer system maximizes the efficiency of PCR amplification, even in the presence of PCR inhibitors > Ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
Figure 1. Taq DNA Polymerase: Robust amplification of GC-rich DNA
Figure 2. Taq DNA HS Polymerase: Sensitive amplification across a 10-fold serial dilution
A serial dilution in duplicate of human genomic DNA (1 μ g-12.5 ng, 1-6 respectively) was used with Taq DNA Polymerase to amplify 61% GC rich fragment human myc gene, to demonstrate the high yield and sensitivity of the Taq DNA Polymerase.
10-fold serial dilution (10 4 copies down to 10 copies) of human DNA using Taq HS DNA Polymerase and Fast qPCR Buffer, 4x (MDX033) to illustrate the speed, sensitivity and reproducibility when using Taq HS DNA Polymerase in a qPCR reaction.
PRODUCT
CAT NO.
VOLUME
REACTIONS
0.1 mL
500 Rxns
MDX001
Taq DNA Polymerase
10 mL
50,000 Rxns
0.1 mL
500 Rxns
MDX008
Taq HS DNA Polymerase
10 mL
50,000 Rxns
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