PCR & qPCR Applications
Aptamer Taq Hot-Start Polymerase (Glycerol-Free)
A high concentration (50 U/ µ L) glycerol‑free Taq DNA Polymerase with a DNA aptamer that serves as a molecular switch to regulate the polymerase’s activity. Unlike traditional hot start methods that require activation for several minutes at very high temperatures, aptamers can immediately disassociate from the polymerase (e.g. activate it) at relatively low temperatures (45 °C). The aptamer reversibly binds to the polymerase and inhibits its activity at room temperature before and after reaction completion, providing tighter control over the start of the PCR reaction and enhancing its efficiency and specificity. Aptamer Taq HS is an ideal polymerase for challenging high-throughput assays where fast reaction protocols and high specificity are required.
> Uses aptamer technology acts as a molecular switch to regulate the polymerase’s activity and reduces assay run time by up to 15 minutes > Convenient room-temperature reaction set-up > Highly suited to multiplex, high-throughput viral detection assays requiring high specificity
Figure 5. Aptamer Taq: Specific, sensitive and reproducible amplification in a multiplex assay
Figure 6. Aptamer Taq: Fast Hot-Start
Four viral sequences, two DNA (Cytomegalovirus ( green line ) and Adenovirus ( blue line )) and two RNA (Rotavirus ( orange line ) and Norovirus ( red line )) were amplified using Glycerol Free Instant Taq HS (MDX015) using Lyo-Ready qPCR Buffer (MDX022) and RNase-Tolerant MMLV-RT (MDX043) in quadruplex qPCR probe assays. The results illustrate that the use of aptamer hot-start gives just as sensitive results with RNA as it does with DNA templates.
Comparison of qPCR performance of Aptamer Taq HS (Glycerol-Free) ( dark blue ) and an antibody hot-start Taq (MDX008) light blue ). qPCR reactions were run with and without an initial 2-minute, high-temperature activation step for both polymerases and Ct values were compared. The data illustrates the immediate activation of the Aptamer Taq HS (Glycerol-Free), with no differences in Ct values with and without an activation step, unlike the antibody hot-start Taq, allowing for faster hot-start and faster reaction protocols without compromising sensitivity.
PRODUCT
CAT NO.
VOLUME
REACTIONS
0.1 mL
500 Rxns
Aptamer Taq HS (Glycerol-Free)
MDX015
10 mL
50,000 Rxns
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