Isothermal Applications
Bst DNA Polymerase
Bst DNA Polymerase, short for Bacillus stearothermophilus DNA Polymerase, is a type of enzyme commonly used in isothermal DNA amplification methods such as Loop-Mediated Isothermal Amplification (LAMP), Isothermal Helicase-Dependent Amplification (HAD) and Isothermal Multiple Cross Displacement Amplification (MCA). Meridian’ Bst DNA Polymerase is derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I and exhibits fast 5´- 3´ DNA polymerase activity and strong strand displacement activity to deliver the fastest amplification speed (time-to-results), yield, salt tolerance, and sensitivity. It is available in two concentrations, 8 U/ µ L or 100 U/ µ L and is supplied with a separate Enzyme Dilution Buffer and a Bst Reaction Buffer for complete assay design flexibility.
> Optimized for isothermal amplification such as LAMP, HAD and MCA > Designed for fast polymerization and quicker time-to-results > Supplied with a separate reaction buffer and enzyme dilution buffer > Available in two concentrations, including a high 100 U/ µ L concentration
Figure 10. Bst DNA Polymerase: Faster time-to-results and better reproducibility compared to competitors
Figure 11. High-concentration Bst DNA Polymerase: Faster time-to-results and better reproducibility compared to competitors
CFTR (cystic fibrosis transmembrane receptor) was screened using High Conc. Glycerol-Free Bst ( orange ), NEB Bst 2.0 ( purple ), NEB wild-type Bst Large Fragment ( green ) and ArcticZymes IsoPol DNA Polymerase ( blue ), using the same test conditions across four technical replicates, to demonstrate the faster time to results (TTR) and better reproducibility of High Conc. Glycerol-Free Bst (MDX018).
A human pathogenic parasite (Strongyloides stercoralis) was screened using Bst Polymerase ( orange ), NEB Bst 2.0 ( purple ) and NEB wild-type Bst Large Fragment ( green ), using the same test conditions across four technical replicates, to demonstrate the faster time to results (TTR) and better reproducibility of Bst Polymerase (MDX012).
PRODUCT
CAT NO.
VOLUME
REACTIONS
1 mL
8,000 Rxns
MDX012
Bst DNA Polymerase
10 mL
80,000 Rxns
0.08 mL
8,000 Rxns
High Conc. Glycerol-Free Bst (100 U/ µ L)
MDX018
0.8 mL
80,000 Rxns
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
Connect with us:
ISO 13485 Certified
08/23
Powered by FlippingBook