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Liquid Stable Flex DNA qPCR Mix (MDX360) High-concentration | Liquid | Long-term ambient-temperature stable
Product Highlights
Simplify your assay development with Meridian’s Liquid Stable Flex DNA qPCR Mix (MDX360), a 5× liquid master mix engineered for long-term ambient stability and workflow adaptability. By eliminating cold chain logistics, thawing, drying and rehydration, MDX360 streamlines assay development and deployment while delivering high sensitivity and reproducibility across diverse assay formats. Designed for flexible oligo addition at the point of use, MDX360 supports customized assay design without compromising stability. Validated for up to 12 months of reliable performance after being stored at 15-25°C without primers and probes, this mix ensures consistent amplification under fast cycling and multiplex conditions. MDX360 empowers developers to simplify logistics, reduce waste and scale confidently with a practical, ambient-stable solution. True Ambient Stability Maintains full performance for up to 12 months at 15-25°C as a liquid, without refrigeration or cold shipping.
• True ambient stability : Maintains full performance for up to 12 months at 15-25°C without refrigeration or cold shipping. • Rapid setup : Simply add primers, probes and template; no thawing required. • Bench-stable after assembly : Once oligos are added, reactions remain stable for up to 1 week at ambient temperature. • Automation compatible : Suitable for both low- and high-throughput liquid handling and automated platforms. • Consistent performance : Delivers high sensitivity, specificity, and broad dynamic range in singleplex and multiplex assays, including fast cycling protocols.
PRODUCT
CAT NO.
VOLUME REACTIONS 0.8 mL 200 Rxns 4 mL 1,000 Rxns 40 mL 10,000 Rxns
Liquid Stable Flex DNA qPCR Mix, 5x
MDX360
Create your assay under ambient-temperature conditions
Customer runs assay at point of test
ADD PRIMERS & PROBES
Run assay
qPCR master mix is shipped at ambient- temperature (15-25 ˚ C)
Aliquot mix into assay
Ship to customer at ambient- temperature (15-25 ˚ C)
Ambient-temperature storage for up to 12 months
Ambient-temperature storage for up to 1 week
www.meridianbioscience.com/lifescience
Performance Data
Maximize qPCR workflow flexibility with our ambient-stable 5x liquid mix – add primers and probes at the point of use, reduce waste, cut costs and eliminate cold storage.
Superior Stability to Comparative Mixes with No Performance Loss Over Time
Performance of qPCR mixes after 1 year at ambient temperature
Performance of qPCR mixes after 1 year at ambient temperature Performance of qPCR mixes after 1 year at ambient temperature Performance of qPCR mixes after 1 year at ambient temperature
Supplier A
MDX360
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MDX360
Supplier A
MDX360
MDX360
Supplier A
Supplier A
–20°C | 22°C (ambient temperature)
Liquid Stable Flex DNA qPCR Mix (MDX360) stored at ambient-temperature was compared to an alternative master mix (from supplier A), stored at –20°C ( blue ) or ambient temperature (22°C) for 12 months ( red ). The multiplexing capacity and sensitivity of both mixes was tested in triplex assays using TaqMan probes and two template dilutions. The results demonstrate that Liquid Stable Flex DNA qPCR Mix stored at –20°C shows no significant loss in performance, as measured by fluorescence intensity and Ct values. In contrast, the qPCR mix from supplier A showed significantly reduced performance under the same conditions. These results indicate that the MDX360 mix remains stable at ambient temperature (22°C) for at least 12 months.
Proven Ambient Stability with Oligos for One Week
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–20°C | 22°C (ambient temperature)
Liquid Stable Flex DNA qPCR Mix (MDX360) was stored at –20°C ( blue ) or ambient temperature (22°C) ( red ) for 1 week with primers and probe and then tested in triplex assays using two template dilutions. The results demonstrate that Liquid Stable Flex DNA qPCR Mix can be stored at ambient temperature for up to a week with oligos without showing significant loss in performance, as measured by fluorescence intensity and Ct values, supporting the use of MDX360 in long, preassembled, high-throughput assays.
Connect with us
USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223 Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com
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www.meridianbioscience.com/lifescience
Liquid Stable Flex DNA qPCR Mix (MDX360) | 09/25
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