Performance Data
Maximize qPCR workflow flexibility with our ambient-stable 5x liquid mix – add primers and probes at the point of use, reduce waste, cut costs and eliminate cold storage.
Superior Stability to Comparative Mixes with No Performance Loss Over Time
Performance of qPCR mixes after 1 year at ambient temperature
Performance of qPCR mixes after 1 year at ambient temperature Performance of qPCR mixes after 1 year at ambient temperature Performance of qPCR mixes after 1 year at ambient temperature
Supplier A
MDX360
100
100
100
80
80
80
60
60
60
40
40
40
20
20
20
0
0
0
MDX360
Supplier A
MDX360
MDX360
Supplier A
Supplier A
–20°C | 22°C (ambient temperature)
Liquid Stable Flex DNA qPCR Mix (MDX360) stored at ambient-temperature was compared to an alternative master mix (from supplier A), stored at –20°C ( blue ) or ambient temperature (22°C) for 12 months ( red ). The multiplexing capacity and sensitivity of both mixes was tested in triplex assays using TaqMan probes and two template dilutions. The results demonstrate that Liquid Stable Flex DNA qPCR Mix stored at –20°C shows no significant loss in performance, as measured by fluorescence intensity and Ct values. In contrast, the qPCR mix from supplier A showed significantly reduced performance under the same conditions. These results indicate that the MDX360 mix remains stable at ambient temperature (22°C) for at least 12 months.
Proven Ambient Stability with Oligos for One Week
Target 1
Target 2
Target 3
–20°C | 22°C (ambient temperature)
Liquid Stable Flex DNA qPCR Mix (MDX360) was stored at –20°C ( blue ) or ambient temperature (22°C) ( red ) for 1 week with primers and probe and then tested in triplex assays using two template dilutions. The results demonstrate that Liquid Stable Flex DNA qPCR Mix can be stored at ambient temperature for up to a week with oligos without showing significant loss in performance, as measured by fluorescence intensity and Ct values, supporting the use of MDX360 in long, preassembled, high-throughput assays.
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Liquid Stable Flex DNA qPCR Mix (MDX360) | 09/25
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