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Liquid Stable Ready DNA qPCR Mix (MDX364) High-concentration | Liquid | Long-term ambient-temperature stable even after adding your primers and probes
Product Highlights
Revolutionize your assay workflow with Meridian’s Liquid Stable Ready DNA qPCR Mix (MDX364) – the only liquid qPCR master mix offering true long- term ambient stability, even after primers and probes are added. MDX364 allows complete reactions to be shipped and stored at room temperature, removing the need for cold chain transport, drying, or rehydration. It maintains sensitivity, speed, and reliability, and has been validated for up to 12 months at 15-25°C, with or without oligos. MDX364 delivers consistent performance under fast cycling and multiplex conditions, while simplifying logistics, enabling confident scale-up, and reducing the risk of false positives. True Assay Stability Maintains full performance for up to 12 months at 15–25°C in liquid form – with or without primers and probes. No refrigeration or cold shipping required.
• Ambient-stable formulation : Retains full qPCR performance for at least 12 months at 15–25°C and for 2 weeks at 50°C, removing the need for cold chain shipping and storage. • Compatible with pre-added oligos : Maintains stability and activity even with primers and probes in the mix. • Liquid, ready-to-use format : Eliminates lyophilization or rehydration steps. • High-concentration 5× mix : Supports reduced reaction volumes and efficient reagent use. • Automation-ready : Designed for liquid handling and high-throughput workflows.
PRODUCT
CAT NO.
VOLUME REACTIONS
0.8 mL
200 Rxns
Liquid Stable Ready DNA qPCR Mix, 5x
MDX364
4 mL 1,000 Rxns 40 mL 10,000 Rxns
Create your assay under ambient-temperature conditions
Customer runs assay at point of test
ADD TEMPLATE
ADD PRIMERS & PROBES
Run assay
qPCR master mix is shipped at ambient- temperature (15-25 ˚ C)
Aliquot mix into assay and ship to customer at ambient-temperature (15-25 ˚ C) *Stable up to 50˚C for 2 weeks
Ambient- temperature storage for up to 12 months
Ambient- temperature storage for up to 3 weeks
www.meridianbioscience.com/lifescience
Performance Data
Stability at Ambient Temperature for 12 Months
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–20°C | 22°C (ambient temperature)
Liquid Stable Ready DNA qPCR Mix (MDX364) was stored at –20°C ( blue ) and at an ambient temperature (22°C) ( red ) for 12 months and then tested in triplex assays with two template dilutions. These results indicate that compared to a frozen master mix stored at –20°C (control), the Liquid Stable Ready DNA qPCR Mix stored at an ambient temperature (22°C) for 12 months showed no significant loss in performance, as measured by fluorescence intensity and Ct values, demonstrating that Liquid Stable Ready DNA qPCR Mix is stable at ambient temperatures (15–25°C) for at least 12 months.
12-Month Ambient Stability with Oligos
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–20°C | 22°C (ambient temperature)
Liquid Stable Ready DNA qPCR Mix (MDX364) was stored at –20°C ( blue ) or at an ambient temperature (22°C) ( red ) for 12 months with primers and probe and then tested in a triplex assay. Results show no significant difference in fluorescence intensity or Ct values compared to the frozen control, confirming that MDX364 pre-assembled with oligonucleotides remains stable at ambient temperatures for at least 12 months.
Stability at 50°C for 2 Weeks
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50C-1 | 50C-2 | 50C-3 | 50C-4 | 50C-5 | F1-frozen | F2-frozen | F3-frozen | F4-frozen | F5-frozen | NC-50C | NTC-frozen
–20°C | 50°C
Liquid Stable Ready DNA qPCR Mix (MDX364) was stored at −20°C ( blue ) or 50°C ( red ) for 2 weeks with primers and probes, then tested in a triplex assay. Compared to the same mix stored at −20°C, MDX364 stored at 50°C showed no significant loss of performance, as indicated by consistent fluorescence intensity and Ct values. This demonstrates that MDX364, pre-assembled with assay oligonucleotides, remains stable even at elevated temperatures.
Connect with us
USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223 Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com
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www.meridianbioscience.com/lifescience
Liquid Stable Ready DNA qPCR Mix (MDX364) | 09/25
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