Performance Data
Stability at Ambient Temperature for 12 Months
Target 1
Target 2
Target 3
–20°C | 22°C (ambient temperature)
Liquid Stable Ready DNA qPCR Mix (MDX364) was stored at –20°C ( blue ) and at an ambient temperature (22°C) ( red ) for 12 months and then tested in triplex assays with two template dilutions. These results indicate that compared to a frozen master mix stored at –20°C (control), the Liquid Stable Ready DNA qPCR Mix stored at an ambient temperature (22°C) for 12 months showed no significant loss in performance, as measured by fluorescence intensity and Ct values, demonstrating that Liquid Stable Ready DNA qPCR Mix is stable at ambient temperatures (15–25°C) for at least 12 months.
12-Month Ambient Stability with Oligos
Target 1
Target 2
Target 3
–20°C | 22°C (ambient temperature)
Liquid Stable Ready DNA qPCR Mix (MDX364) was stored at –20°C ( blue ) or at an ambient temperature (22°C) ( red ) for 12 months with primers and probe and then tested in a triplex assay. Results show no significant difference in fluorescence intensity or Ct values compared to the frozen control, confirming that MDX364 pre-assembled with oligonucleotides remains stable at ambient temperatures for at least 12 months.
Stability at 50°C for 2 Weeks
Target 1
Target 2
Target 3
50C-1 | 50C-2 | 50C-3 | 50C-4 | 50C-5 | F1-frozen | F2-frozen | F3-frozen | F4-frozen | F5-frozen | NC-50C | NTC-frozen
–20°C | 50°C
Liquid Stable Ready DNA qPCR Mix (MDX364) was stored at −20°C ( blue ) or 50°C ( red ) for 2 weeks with primers and probes, then tested in a triplex assay. Compared to the same mix stored at −20°C, MDX364 stored at 50°C showed no significant loss of performance, as indicated by consistent fluorescence intensity and Ct values. This demonstrates that MDX364, pre-assembled with assay oligonucleotides, remains stable even at elevated temperatures.
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Liquid Stable Ready DNA qPCR Mix (MDX364) | 09/25
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