High Inhibitor Tolerance in the Presence of Whole Blood K2-EDTA or Plasma
(A) Lyo-Ready™ DNA LAMP Blood (MDX124)
HBV with 5% blood
60
50
40
30
20
10
0
HBV + 5% Blood
EBV + 20% Blood
P. malaria + 5% Blood
HSV2 + 20% Plasma
M. pneumoniae + 5% Blood
Lyo-Ready ™ Direct DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™
Dengue 1 with 10% blood
(B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
50
40
30
20
10
0
Dengue 1 + 10% Blood
HCV + 10% Blood
WNV + 5% Blood
ZIKV + 5% Blood
HIV-1 Plasma extract
Lyo-Ready ™ Direct RNA/DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™
(A) Lyo-Ready ™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for either HSV-2, EBV, HBV, Plasmodium malaria and Mycoplasma pneumonia and (B) Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for either Dengue Type-1, HCV, WNV, Zika or HIV-1. The performance of the mixes were compared against liquid formats of NEB WarmStart ® LAMP kit ( orange ) and Thermo SuperScript ™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-20% Blood K2-EDTA or plasma extract. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that both MDX124 and MDX125 have earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready ™ Direct DNA LAMP Blood (MDX124) and Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125) perform better and with faster amplification across the range of inhibitors compared to the other mixes.
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