Meridian Lyo-Ready Direct DNA LAMP Blood Mixes

High Inhibitor Tolerance in the Presence of Whole Blood K2-EDTA or Plasma

(A) Lyo-Ready™ DNA LAMP Blood (MDX124)

HBV with 5% blood

60

50

40

30

20

10

0

HBV + 5% Blood

EBV + 20% Blood

P. malaria + 5% Blood

HSV2 + 20% Plasma

M. pneumoniae + 5% Blood

Lyo-Ready ™ Direct DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™

Dengue 1 with 10% blood

(B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)

60

50

40

30

20

10

0

Dengue 1 + 10% Blood

HCV + 10% Blood

WNV + 5% Blood

ZIKV + 5% Blood

HIV-1 Plasma extract

Lyo-Ready ™ Direct RNA/DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™

(A) Lyo-Ready ™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for either HSV-2, EBV, HBV, Plasmodium malaria and Mycoplasma pneumonia and (B) Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for either Dengue Type-1, HCV, WNV, Zika or HIV-1. The performance of the mixes were compared against liquid formats of NEB WarmStart ® LAMP kit ( orange ) and Thermo SuperScript ™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-20% Blood K2-EDTA or plasma extract. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that both MDX124 and MDX125 have earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready ™ Direct DNA LAMP Blood (MDX124) and Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125) perform better and with faster amplification across the range of inhibitors compared to the other mixes.

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