Product Spotlights
TetroScript Reverse Transcriptase The only Reverse Transcriptase with comparable performance to SuperScript
TetroScript Reverse Transcriptase is a high concentration, robust thermostable Moloney Murine Leukemia Virus- Reverse Transcriptase (MMLV-RT), designed to reduce RNase H activity and increase thermal stability. The enzyme offers higher cDNA yields and improved efficiency on GC-rich target RNAs and RNA with high secondary structure, such as viral RNA. Key Features: • High thermostability: reverse transcriptase activity up to 60°C, ideal for RNA with high secondary structure such as viral genomes • Higher enzyme efficiency and sensitivity: reliable quantification of low abundance targets and scarce samples • Low glycerol: formulation compatible with lyophilization and air-drying applications • Fast: delivers reproducible, multiplex accurate RT-qPCR assay results in as little as 30 minutes
TetroScript vs Superscript – thermostability comparison
Performance of TetroScript Reverse Transcriptase ( red ), standard MMLV-RT ( grey ) and SuperScript III (ThermoFisher) ( black ) after pre-incubation at 40°C to 70°C for 10 mins, in a multiplex one-step RT-qPCR assay. Δct values were calculated against the ct value produced by the same enzyme stored at-20 °C. The data illustrates the increased thermal stability of TetroScript Reverse Transcriptase when compared to other MMLV-RT enzymes and its ability to efficiently synthesize cDNA at temperatures up to 60°C.
Compatible with lyophilization and air-drying TetroScript Reverse Transcriptase proprietary formulation allows for incorporation into assays designed for subsequent lyophilization.
Retains the same activity after drying
Air-dried | Wet
TetroScript Reverse Transcriptase was used in an air-dryable RT-qPCR Mix and dried down in a fan assisted oven ( red ). After rehydration, the mix was tested against freshly prepared liquid mix ( blue ), in a triplex one-step RT-qPCR assay on respiratory RNA virus targets (Influenza A, MERS-CoV and RSV). The results demonstrate that TetroScript Reverse Transcriptase retains the same activity level after air-drying even in challenging conditions such as multiplex RT-qPCR reactions.
Applications: The high-quality first-strand cDNA generated makes TetroScript Reverse Transcriptase ideal for: • Construction of cDNA libraries • 2-Step qPCR assays • Viral RNA targets • Gene cloning
PRODUCT
CAT NO.
REACTIONS
BIO-65111 10,000 Units BIO-65112 4 x 10,000 Units
TetroScript Reverse Transcriptase (200 U/μL)
To review the product in detail or download our flyer, visit: https://www.bioline.com/thermostable-reverse-transcriptase.html
www.bioline.com
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