SensiFAST TM Probe Direct SuperMix Advanced mix for direct DNA amplification from crude samples without extraction SensiFAST™ Probe Direct SuperMix is a highly inhibitor-resistant qPCR master mix that provides quick and easy extraction and amplification of DNA from a variety of tissue types. The SuperMix maximizes sensitivity while simultaneously minimizing the effect of blood, tissue and plant PCR inhibitors, to deliver greater experiment success rates. Key Features: • Inhibitor tolerant: optimized proprietary buffer system designed for overcoming common PCR inhibitors in crude lysate or unprocessed blood, tissue and plant samples • Specific: antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability • Sensitive: reliable quantification of low abundance targets and scarce samples • Reproducible: consistent results between technical replicates for increased confidence in results • Fast: delivers reproducible, multiplex accurate assay results in as little as 30 minutes High qPCR efficiency across a range of inhibitors SensiFAST™ Probe Direct SuperMix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, dNTPs and MgCl 2 . It has been designed for highly reproducible, accurate assay results in the presence of inhibitors, making it ideal for direct amplification from the most challenging samples. SensiFAST™ Probe Direct SuperMix has been tested with a variety of crude samples known to inhibit PCR, from whole blood to proteinase lysed crude DNA extracts, making it ideal for everything from biofluids to animal and environmental samples.
Efficient amplification from different sample types
SensiFAST Probe Direct SuperMix | Roche
Applications: Eliminating costly, time-consuming, cumbersome purification procedures that can lead to sample loss, not only increase the speed and sensitivity of the assay, but also makes SensiFAST™ Probe Direct SuperMix especially valuable for high-throughput applications with limited starting material, such as gene expression experiments, making it ideal for A 10-fold serial dilution of genomic DNA was spiked into cerebrospinal fluid, human urine and cow whole milk was amplified, using SensiFAST™ Probe Direct SuperMix ( green ) and Kapa Probe Force (Roche) ( red ) and the manufacturers’ recommended protocol. The results illustrate Sensi- FAST™ Probe Direct SuperMix is more sensitive than Kapa Probe Force, as lower dilutions could be detected, with better efficiencies for all three inhibitor samples.
• Gene expression analysis • Viral and bacterial detection • GMO testing • SNP genotyping • Mutation detection • Environmental monitoring
PRODUCT
CAT NO.
REACTIONS
BIO-86105 500 Reactions BIO-86120 2,000 Reactions
SensiFAST™ Probe Direct SuperMix
To review the product in detail or download our flyer, visit: https://www.bioline.com/sensifast-probe-direct-supermix.html
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