Superior reaction efficiency using crude urine samples in multiplex reactions
Lyo-Ready™ Direct DNA qPCR Urine (MDX152)
Mycoplasma genitalium
Treponema pallidum
Chlamydia trachomatis
Neisseria gonorrheae
MDX152 | Quantabio ToughMix | Roche KAPA | ThermoFisher TaqPath ™
Four sexually transmitted pathogens ( M. genitalium, T. pallidum (Syphilis), C. trachomatis and N. gonorrhoeae ) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready ™ Direct DNA qPCR Urine in a lyophilized format ( red ) and compared to Quantabio ToughMix PCR Master Mix ( grey ), Roche KAPA Probe Force qPCR kit ( green ) and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ) in a liquid format. The results demonstrate that Lyo-Ready ™ Direct DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.
Lyo-Ready™ Direct RNA/DNA qPCR Urine (MDX153)
Dengue
Zika
Chikungunya
Malaria
MDX153 | ThermoFisher TaqPath ™ | Quantabio ToughMix | BioRad Reliance 1-Step
Three viral RNA (Dengue, Zika and Chikungunya) and one DNA (Malaria) mosquito-borne infectious disease targets were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready ™ Direct RNA/DNA qPCR Urine in a lyophilized format ( red ) and compared to ThermoFisher TaqPath ™ 1-Step Multiplex Master Mix ( black ), Quantabio UltraPlex 1-Step ToughMix ™ ( grey ) and BioRad Reliance 1-Step Multiplex RT-qPCR Supermix ™ ( pink ) in liquid formats. The results illustrate that dry Lyo-Ready ™ Direct RNA/DNA qPCR Urine has higher multiplexing capacity and reproducibility than the liquid competitor mixes.
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