Meridian Lyo-Ready Direct qPCR Urine flyer 0922

High amplification sensitivity with low copy templates using either liquid and dried-down assay formats

A) MDX152

B) MDX153

10,000 copies

1,000 copies

100 copies

10 copies

Lyophilized | Liquid

Activity of A) Lyo-Ready ™ Direct DNA qPCR Urine in lyophilized ( red ) and liquid ( blue ) format was compared in the ability to amplify a 10-fold serial dilution of synthetic DNA (10,000, 1,000, 100 and 10 copies respectively) and B) Activity of Lyo-Ready ™ Direct RNA/DNA qPCR Urine in lyophilized ( red ) and wet ( blue ) format was compared in the ability to amplify a 10-fold synthetic RNA target (10,000, 1,000, 100 and 10 copies respectively) in the presence of 10% pooled human urine. The results illustrate that the dried mix retains the ability to efficiently amplified to the same level as the wet mix with the same level of sensitivity and reproducibility for both.

Fast and more sensitive detection of cancer markers in urine samples

CDC2

IGFBP5

MDK

Three tumor-related mRNA markers (CDC2 kinase, IGFBP5 and MDK) were amplified from total RNA (from a bladder cancer patient) in a triplex reaction using lyophilized Lyo-Ready ™ Direct RNA/DNA qPCR Urine ( red ) or liquid BioRad Reliance One-Step Multiplex Supermix ™ ( pink ) in the presence of 10% Human Urine. The results illustrate that Lyo-Ready ™ Direct RNA/DNA qPCR Urine is able to detect cancer related RNA markers from urine faster and with higher sensitivity compared to the other mix.

MDX153 | BioRad Reliance 1-Step