Compatible with very fast cycling conditions to deliver very fast results
A/ Lyo-Ready™ Direct DNA qPCR Urine (MDX152)
A) Targets from Neisseria gonorrhoeae , Chlamydia trachomatis and group B streptococcus (GBS) were amplified in a triplex reaction in the presence of 25% human urine under standard cycling conditions: 40 cycles of 10 sec @ 95°C and 30 sec @ 60°C and fast cycling conditions: 3 min at 95°C followed by 40 cycles of 1 sec @ 95°C and 1 sec @ 60°C. B) Three mammalian RNA targets were amplified in a triplex reaction in the presence of 5% human urine under standard cycling conditions: 10 min reverse transcription step followed by 40 cycles of 10 sec @ 95°C and 30 sec @ 60°C and fast cycling conditions: 5 min reverse transcription step followed by 40 cycles of 1 sec @ 95°C and 1 sec @ 60°C. All reactions were carried out in the Quantstudio ™ 7 Flex Real-Time PCR System. The results illustrate the ability of Lyo-Ready Direct Urine mixes to deliver the fastest possible results, without compromising the sensitivity and reproducibility of an assay.
B/ Lyo-Ready™ Direct RNA/DNA qPCR Urine (MDX153)
Extended shelf-life of greater than 12 months at ambient temperature
A) MDX152
B) MDX153
Lyophilized | Liquid
Lyo-Ready ™ Direct DNA qPCR Urine and Lyo-Ready ™ Direct RNA/DNA qPCR Urine were lyophilized, and the stability tested in accelerated stability studies. The lyophilized mixes ( red ) were incubated at 37°C for 1 month and tested against the fresh liquid mixes ( blue ) in a triplex qPCR assay with 10% human urine (MDX152) and a triplex RT-qPCR assay with 5% human urine (MDX153). Results suggest that the lyophilized mix is active following accelerated stability tests with projected at least 12 months stability at ambient temperature.
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