Air-Dryable RT-qPCR and qPCR mixes
S imple and easy to use Ideal for multiplex assays on POC diagnostic platforms or automated high-throughput instruments C ompatible with a range of air-drying protocols to produce an ambient temperature stable mix R educes cost and complexity
PRODUCT
CAT NO.
VOLUME REACTIONS
5 mL
1,000 Rxn
Air-Dryable™ 1-Step RT-qPCR Mix, 4x
MDX095
50 mL
10,000 Rxn
5 mL
1,000 Rxn
Air-Dryable™ qPCR Mix, 4x
MDX082
50 mL
10,000 Rxn
RT-qPCR Data Highlights
High-tolerance to PCR inhibitors in sputum Amplification profile of a mouse RNA target spiked into samples containing 10%, 5% or 0% artificial sputum in Universal Transport Media (UTM). The data illustrates that the performance of Air-Dryable ™ 1-Step RT-qPCR Mix (MDX095) exhibits high tolerance towards inhibitors present in artificial sputum and UTM.
Full enzyme activity following air-drying Activity of Air-Dryable ™ 1-Step RT-qPCR Mix in both wet and air-dried formats were compared on 10-fold dilution mouse RNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mix up to the assay limit of detection.
0% UTM+Sputum 5% UTM+Sputum 10% UTM+Sputum
Wet | Air-dried
High efficiency and sensitivity in multiplex reactions
Amplification profiles of different RNA viruses (Influenza A, Norovirus and Rotavirus) in a multiplex reaction. The results demonstrate the increased performance of air-dried MDX095 ( red ) against a standard wet RT-qPCR Mix ( black ) for the detection of viral RNA.
Influenza A
Novovirus
Rotavirus
Air-dried | Wet
Air-dried | Wet
Air-dried | Wet
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