Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety.
Mouse-FREE IgG An animal-free, sustainable alternative to serum-derived mouse IgG
Immunoassays that use animal-derived products can have batch-to-batch variation, low specificity, high background and interference from cross-reactive proteins — all of which can compromise assay performance. Recombinant technologies have enabled animal-free alternatives that offer greater reproducibility, scale-up and alleviate ethical concerns. The European Union Directive 2010/63/EU encourages the use of animal –free reagents. Companies that truly care about sustainability and the environment are encouraging scientist to minimize the use of animal-derived products in their work. Mouse-FREE IgG (Cat# BN1300) is a recombinant HAMA blocker made from a proprietary mixture of full-length antibodies present in native mouse IgG (IgG1, IgG2a, IgG2b, IgG3). Mouse-FREE IgG is a direct replacement to the animal version and it has the same HAMA blocking efficiency.
HAMA Blocking Effectiveness - Mouse-FREE IgG vs. Native Mouse Serum
0.035
Cruelty free Animal free
Same HAMA blocking potency as native version
Sustainable commercial-scale manufacturing
Economically Feasible
Supply continuity
0.03
0.025
0.02
Mouse-FREE IgG (Cat# BN1300)
0.015
Product Highlights • Recombinant mixture of full-length antibody isotypes present in native Mouse IgG. • Slightly better HAMA blocking performance when compared to native version. • No animal products used in the manufacturing process
0.01
Did you know? 1kg of Mouse IgG is produced from 80,000 mice.
0.005
0
Mouse-FREE IgG
Native Mouse IgG
Human HAMA-positive serum was incubated with Meridian’s Mouse-FREE IgG (Cat No. BN1300) or serum-derived purified native mouse IgG at concentrations ranging from 0.0001-1.25 µ g/mL for 20 mins at room temperature. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA signal – the lower EC50 values indicate greater interference suppression.
www.meridianbioscience.com/lifescience
Interfering HA (e.g. HAMA) Mouse IgG
Mouse IgG
Why use blockers such as Mouse IgG?
IgM RF
Blockers are used in ELISA and LF assays to reduce interference from proteins in patient samples that could produce false results and an incorrect diagnosis. Specifically in double mouse monoclonal assays, blockers are required to remove particular types of HA interference called human anti-mouse antibodies (HAMA) and Rheumatoid Factor (RF). Passive blocking reagents such as Mouse IgG work by preventing interfering antibodies from binding to the capture or detection antibodies by providing alternate binding sites. Mouse IgG must be added in excess concentration and the effectiveness depends on the affinity of interfering antibody for the animal IgG.
Detection Antibody (mouse origin) Capture Antibody (mouse origin) Antigen
Blocking Buffer
Figure 2. Blocking antibodies such as mouse IgG neutralize the interfering antibodies present in serum samples resulting in accurate binding of the analyte of interest by the capture and detection antibodies.
Mouse-FREE IgG Performance Data
A) HAMA Interference
100
100
100
80
80
69
66
60
60
42
40
40
20
20
0
0
Mouse-FREE IgG
Native Mouse IgG
Competitor Blocker #1
Competitor Blocker #2
Blocker concentration ( µ g/ml)
B) RF Interference
100
100
100
80
80
60
60
40
40
20
20
14
14
11
0
0
Mouse-FREE IgG
Native Mouse IgG
Competitor Blocker #1
Competitor Blocker #2
0.01
0.1
1
10
100
Blocker concentration ( µ g/ml)
Native mouse IgG | Mouse-FREE IgG | Competitor Blocker #1 | Competitor Blocker #2
Figure 3. A double mouse monoclonal sandwich assay was used to compare the performance of Meridian’s Mouse-FREE IgG (100% animal-free, recombinant Mouse IgG, Cat No. BN1300) against serum-derived purified native mouse IgG and two competitor HAMA blockers. (A) Human HAMA-positive or (B) RF-positive serum was preincubated with the individual blockers for 20 minutes at room temperature. Blocker concentrations ranged from 0.0003-1.25 μ g/mL for the HAMA assay and 0.0195-80 μ g/mL for the RF assay. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA or RF signal – lower EC50 values indicate greater interference suppression. The results demonstrate that Mouse-Free IgG is significantly more effective than native mouse IgG and the two competitor blockers at blocking HAMA and RF interference.
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
Connect with us:
ISO 13485 Certified
06/23
Page 1 Page 2Powered by FlippingBook