Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or Universal Transport Media (up to 35% UTM)
Air-Dryable ™ Direct DNA qPCR Saliva
Mycoplasma pneumoniae
ADV
CMV
0% human saliva | 10% human saliva | 20% human saliva | 60% human saliva
Mycoplasma pneumoniae genomic DNA, adenovirus (ADV) and cytomegalovirus (CMV) were amplified in a triplex qPCR assay in the presence of 0% ( black ), 10% ( red ), 20% ( blue ) and 60% ( grey ) homogenized human saliva. The results illustrate that the performance of the air-dried, Air-Dryable Direct DNA qPCR Saliva is not affected by increasing concentrations of human saliva.
Air-Dryable ™ Direct RNA/DNA qPCR Saliva
Influenza A
MERS-CoV
RSV
Air-dried | TaqPath ™ (Thermo) | Ultraplex ™ (QuantBio)
Three respiratory pathogens, Influenza A, Middle East Respiratory syndrome coronavirus (MERS-CoV) and Respiratory Syncytial Virus (RSV) were amplified in a triplex qPCR assay in the presence of 35% Universal Transport Media (UTM) with artificial sputum swab. The results illustrate that a higher performance was achieved with Air-Dryable ™ Direct RNA/DNA qPCR Saliva ( red ) compared to the inhibitor-tolerant RT-qPCR mixes TaqPath ™ (Thermo, black ) or Ultraplex ™ (QuantBio, grey ).
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