Air-Dryable Direct Saliva

Efficient Amplification using the mix in a wet or dried down format

Air-Dryable ™ Direct DNA qPCR Saliva

Air-Dryable ™ Direct DNA qPCR Saliva was used in a 10-fold serial dilution of Epstein Barr virus target (80,000, 8,000, 800 and 80 copies respectively), in presence of 20% artificial sputum in both liquid ( blue ) and air-dried ( red ) formats. The results illustrate that the dried mix retains the ability to efficiently amplified to the same level as the wet mix with the same level of sensitivity and reproducibility.

80,000 copies 800 copies 8,000 copies 80 copies

Air-dried | Liquid

Air-Dryable ™ Direct RNA/DNA qPCR Saliva

Air-Dryable ™ Direct DNA/RNA qPCR Saliva was used in a 10-fold serial dilution of VLP-RNA Extraction Control MDX068 (10,000, 1,000, 100 and 10 copies respectively), in the presence of 5% artificial sputum in both liquid ( blue ) and air-dried ( red ) formats. The results illustrate that the dried mix retains the ability to efficiently amplify to the same level as the wet mix with the same level of sensitivity and reproducibility.

10,000 copies 100 copies 1,000 copies 10 copies

Air-dried | Liquid

High performance using standard or fast cycling conditions for greater assay flexibility and reduced run times

Air-Dryable ™ Direct DNA qPCR Saliva Standard cycling

Fast cycling

Mycobacterium tuberculosis DNA target was amplified using standard qPCR cycling conditions and the fastest cycling conditions available on a Quantstudio ™ 7 Flex Real- Time PCR System in the presence of 20% artificial sputum. The results illustrate the higher performance and end-fluorescence of Air-Dryable Direct DNA qPCR Saliva ( blue ) in comparison to mixes from KAPA Probe Force (Roche, green ) and Ultraplex ™ (QuantBio, grey ) in both standard (3 min @ 95ºC, 40 cycles of 10 sec @ 95ºC and 25 sec @ 60ºC) and fast (3 min @ 95ºC, 40 cycles of 1 sec @ 95ºC and 1 sec @ 60ºC) qPCR cycling conditions.

MDX130 | KAPA Probe Force (Roche) | Ultraplex ™ (QuantBio)