Product Information
Quality Stringent control systems guarantee that Meridian’s dNTPs are of the highest quality and consistency to be found anywhere in the industry. A further assurance of this is the ISO certification of our purpose-built state-of-the-art nucleotide manufacturing facilities in the Luckenwalde Biotechnology Park, Berlin-Brandenburg Life Science Cluster, Germany. With ISO certification implemented, Meridian demonstrates its commitment to being a reliable and techni- cally competent partner, for our customers, suppliers and future collaborators. With increasing level of sophistication of molecular processes and regulatory requirements, it has become essential to achieve highest dNTP quality, with minimal batch-to-batch variations. Meridian’s ultra-high purity deoxynucleoside triphosphates (dNTPs) are manufactured to the highest purity standards and perfectly suited for a wide range of applications from PCR (including low copy, long range, qPCR), isothermal amplification, cDNA synthesis/ reverse transcription to next-generation sequencing (NGS). Purity Purity of dNTPs is an essential starting parameter for any highly sensitive diagnostic assays. Inhibitors and contaminants generally exert their effects through negative interaction with template, interference with enzymes such as DNA polymerases, or by other means, such as sequestration of essential co-factors. These can individually contribute to reducing the efficiency of the assay and cause it to fail overall. Meridian’s ultra-pure dNTPs are manufactured to the highest purity standards in the industry. Our dNTPs are enzymatically synthesized (Fig. 1) from premium quality raw materials, using highly specific production systems in our purpose-built facilities. The manufacturing process eliminates trace amounts of impurities and inhibitors that can affect performance. These include: • dNDPs (deoxynucleotide diphosphates) and dNMPs (deoxynucleotide monophosphates), that can act as competitive inhibitors of enzymes, leading to reduced extension efficiency (Fig. 2). • Modified dNTPs (e.g., oxidized or deaminated forms), leading to lead to base misincorporation and mutations. • Residual chemical contaminants from synthesis, these include protecting groups and organic solvents that can inhibit or denature enzyme activity and reduce efficiency.
• DNase and RNase contaminants that can affect cDNA synthesis (Fig. 3). • Nickases and proteases, which can compromise the template (Fig. 4).
Fig.1 Enzymatic synthesis of dNTP Monophosphorylated nucleotides (dNMPs) are converted to deoxynucleotide diphosphates (dNDPs) and then nucleoside triphosphates (dNTPs) using kinase proteins. The synthesized dNTPs can then be used directly in nucleic acid amplification.
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