Product Specification Table dATP
dCTP
dGTP
dTTP
dUTP
dATP 100 mM solution dCTP 100 mM solution dGTP 100 mM solution dTTP 100 mM solution dUTP 100 mM solution
Product
2’-deoxyadenosine – 5’-triphosphate
2’-deoxycytidine – 5’-triphosphate
2’-deoxyguanosine-5’- triphosphate
2’-deoxythymidine-5’- triphosphate
2’-deoxyuridine-5’- triphosphate
Nomenclature
C 10 H 12 N 5 O 12 P 3 * 514.9 g/mol 100 mM ± 5%
C 9 H 12 N 3 O 13 P 3 * 490.9 g/mol 100 mM ± 5%
C 10 H 12 N 5 O 13 P 3 * 530.9 g/mol 100 mM ± 5%
C 10 H 13 N 2 O 14 P 3 * 505.9 g/mol 100 mM ± 5%
C 9 H 12 N 2 O 14 P 3 * 492.884 g/mol 100 mM ± 5%
Formula
Molecular Weight
Concentration Appearance
Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution
7.5 7.0
7.5 7.0
7.5 7.0
7.5 7.0
7.5 7.0
pH of Lithium Solution pH of Sodium Solution
Purity (HPLC)
≥ 99%
≥ 99%
≥ 99%
≥ 99%
≥ 99%
dNDP (HPLC Area)
< 1%
< 1%
< 1%
< 1%
< 1%
Negative 259 nm
Negative 272 nm
Negative 252 nm
Negative
Negative 262 nm
DNase, RNase
267 nm
λ max pH 7.0
15.4 E x mmol -1 x cm -1
9.1 E x mmol -1 x cm -1
13.7 E x mmol -1 x cm -1
9.6 E x mmol -1 x cm -1
10.0 E x mmol -1 x cm -1
ε at λ max at pH 7.0
0.78 ± 0.03 0.15 ± 0.02
0.82 ± 0.03 0.98 ± 0.02
1.16 ± 0.05 0.66 ± 0.02
0.65 ± 0.03 0.73 ± 0.02
0.75 ± 0.03 0.38 ± 0.02
A 250 /A 260 A 280 /A 260 Storage Stability
-20°C
-20°C
-20°C
-20°C
-20°C
≤ 24 months
≤ 24 months
≤ 24 months
≤ 24 months
≤ 24 months
*All dNTP formulations are designed for convenience and flexibility. Individual nucleotides (dATP, dCTP, dGTP, dTTP and dUTP) are supplied in lithium (Li 4 ) or sodium (Na 3 ) salts. Applications Meridian ultra-pure dNTPs possess the highest level of purity in the industry and have been extensively validated for use in a wide variety of molecular biology applications. Validated applications PCR, RT-PCR qPCR, RT-qPCR Long-Range & High-Fidelity PCR cDNA synthesis Isothermal amplification Cloning Next-generation sequencing Sanger sequencing Genotyping Stability and Storage Applications Stability and Storage Meridian ultra-pure dNTPs are available in sodium salts (pH 7.0) or lithium salts (pH 7.5) in highly purified water, to ensure functionality and long-term stability. Our QC results demonstrate that Meridian’s dNTP functionality is not only retained after multiple freeze-thaw cycles but can also greatly exceed the standard 2-year storage recommendation time in a -20°C constant-temperature freezer.
A
Batch 1
Batch 2
Fig. 5 Meridian’s dNTP mix remains stable after multiple rounds of freeze-thawing and long-term storage. A) Two different batches of Meridian 100 mM dNTP mix were freeze-thawed up to 20 times and a two-fold serial dilution was used in a PCR reaction with 10 ng of λ DNA and Taq DNA polymerase to amplify a 2 kb fragment. Reactions with both batches produced identical PCR products, whether freeze-thawed once or multiple times. B) Stability of dNTPs were also assayed for PCR functionality for dNTPs stored at -20°C for up to two years. All dNTP batches performed exceptionally in PCR reactions with λ DNA (10 ng) and amplified with Taq DNA polymerase regardless of age of the dNTPs.
5 cycles
B
10 cycles
Year 0
15 cycles
Year 2
20 cycles
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