Reagent Solutions for Molecular Diagnostics PCR/RT-PCR Enzymes, Optimized Mixes and dNTPs
MOLECULAR REAGENTS FOR MDX APPLICATIONS
BLOOD BANKING
HUMAN DIAGNOSTICS
VET HEALTH
GENETIC SCREENING
FOOD TESTING
ENVIRONMENTAL
DNA BARCODING
WATER TESTING
Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety. Products include PCR and RT-PCR enzymes, ultra-pure dNTPs and buffer systems for IVD assay development and optimization. In addition to these reagents, Meridian has also developed a range of ready-to-use solutions for molecular diagnostic manufacturers. These include enzymes and mixes formulated for lyophilization, internal quality controls to monitor extraction and co-purification of inhibitor in qPCR/RT-qPCR assays as well as a unique mix designed for direct qPCR from crude lysates.
www.MeridianLifeScience.com
Product Selection Chart
• Suitable •• Recommended
••
••
••
Low DNA qPCR Mix
MDX030
Chemical
•• ••
••
Fast qPCR Mix
MDX020
Antibody
MDX031 & MDX060
• ••
Hi-Throughput dUTP Mix
Antibody
•• ••
Inhibitor-Tolerant qPCR Mix
MDX013
Antibody
•• •• •• •• ••
Inhibitor-Tolerant RT-qPCR Mix
MDX016
Antibody
•• ••
Fast 1-Step RT-qPCR Mix
MDX032
Antibody
•• •• ••
Low LOD 1-Step RT-qPCR Mix
MDX025
Antibody
••
• •
Lyo-Compatible MMLV-RT
MDX042
No
• ••
RNase-Tolerant MMLV-RT
MDX043
No
• •
MMLV-RT
MDX044
No
••
•• ••
•
Air-Dryable ™ qPCR Mix
MDX082
Antibody
••
•• •• • •
Air-Dryable ™ 1-Step RT-qPCR Mix
MDX095
Antibody
••
•• •• •• •
Air-Dryable ™ Direct DNA qPCR Blood
MDX092
Antibody
MDX021 & MDX023
••
•• ••
•
Lyo-Ready ™ qPCR Mix
Antibody
••
• ••
Lyo-Ready ™ 1-Step RT-qPCR Mix
MDX024
Antibody
••
• ••
Lyo-Ready ™ 1-Step RT-qPCR Virus Mix
MDX062
Antibody
••
••
•• ••
Lyo-Ready ™ LAMP Mix
MDX097
No
••
••
Bst DNA Polymerase
MDX012
No
••
••
•
High Conc. Glycerol-Free Bst
MDX018
No
• • •
Taq DNA Polymerase
MDX001
No
•• •• •• ••
Taq HS DNA Polymerase
MDX008
Antibody
••
•• •
Glycerol-Free Taq HS
MDX011
Antibody
••
•• ••
••
Aptamer Taq HS (Glycerol-Free)
MDX015
Aptamer
MDX009 & MDX010
••
••
Low DNA Taq HS
Chemical
••
High-Fidelity Pfu
MDX003
No
••
•• ••
High-Specificity Pfu HS
MDX006
Aptamer
2
Optimized Mixes
PRODUCT
APPLICATION CAT NO. VOLUME REACTIONS
qPCR (DNA Template) Hot-Start Master Mixes Low DNA qPCR Mix Ideal for microbial and fungal DNA detection assays.
5 mL
500 Rxn
MDX030
100 mL
10,000 Rxn
5 mL
500 Rxn
Fast qPCR Mix Suitable for fast high-multiplex qPCR assays.
MDX020
100 mL
10,000 Rxn
2 mL
500 Rxn
Fast qPCR Mix, 5x Suitable for fast high-multiplex qPCR assays (5x concentration).
MDX072
40 mL
10,000 Rxn
2 mL
500 Rxn
Digital PCR Mix, 5x Suitable for digital PCR (dPCR) assays (5x concentration).
MDX074
40 mL
10,000 Rxn
Hi-throughput dUTP qPCR Mix Optimized formulation for efficient dUTP incorporation to remove background PCR product contamination. Inhibitor-Tolerant qPCR Mix Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant qPCR Mix, 5x Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants (5x concentration). RT-qPCR (RNA Template) Hot-Start Master Mixes Fast 1-Step RT-qPCR Mix RT-qPCR mix formulated for fast, automated, high-throughput systems. Inhibitor-Tolerant RT-qPCR Mix, 4x All-in-one, 4x master mix designed for RT-qPCR from crude samples such as sputum, stool and saliva. No sample extraction required. Low LOD 1-Step RT-qPCR Mix Suitable for detecting RNA and DNA viruses at very low levels for applications such as blood bank or transplant viral testing.
5 mL 500 Rxn 100 mL 10,000 Rxn 500 Rxn 100 mL 10,000 Rxn 5 mL
MDX031
MDX013
2 mL
500 Rxn
MDX073
40 mL
10,000 Rxn
10 mL 1,000 Rxn 100 mL 10,000 Rxn 200 Rxn 50 mL 10,000 Rxn 1 mL 10 mL 1,000 Rxn 100 mL 10,000 Rxn
MDX032
MDX016
MDX025
Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes Air-Dryable ™ qPCR Mix, 4x Ready-to-use qPCR mix ideal for oven or air-drying technologies.
5 mL 1,000 Rxn 50 mL 10,000 Rxn
MDX082
5 mL
1,000 Rxn
Air-Dryable ™ RT-qPCR Mix, 4x Ready-to-use RT-qPCR Mix ideal for oven or air drying technologies. Air-Dryable ™ Direct DNA qPCR Blood, 4x Inhibitor-tolerant qPCR mix ideal for oven or air-drying technologies. Can be used for direct detection using whole blood, serum or plasma samples. Lyo-Ready ™ qPCR Mix Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.
MDX095
50 mL
10,000 Rxn
5 mL
1,000 Rxn
MDX092
50 mL
10,000 Rxn
5 mL
500 Rxn
MDX021
100 mL
10,000 Rxn
Human Diagnostics Environmental
Vet Health Water Testing
Blood Banking Food Testing
Genetic Screening DNA Barcoding
www.MeridianLifeScience.com 3
Optimized Mixes continued
PRODUCT
APPLICATION
CAT NO. VOLUME REACTIONS
Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes Continued Lyo-Ready ™ qPCR Mix, 2.6x
8 mL
1,000 Rxn
MDX023
Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets (2.6x concentration). Lyo-Ready ™ 1-Step RT-qPCR Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ 1-Step RT-qPCR Virus Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients and highly suited for amplification of RNA viruses with a high secondary structure. Loop-Mediated Isothermal Amplification (LAMP) Master Mixes Lyo-ready ™ LAMP Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.
100 mL 12,500 Rxn
10 mL
1,000 Rxn
MDX024
100 mL
10,000 Rxn
10 mL
1,000 Rxn
MDX062
100 mL
10,000 Rxn
5 mL
800 Rxn
MDX097
50 mL
8,000 Rxn
Polymerases
PRODUCT
APPLICATION
CAT NO. VOLUME REACTIONS
DNA Polymerases
100 µL
500 Units
Taq DNA Polymerase DNA Polymerase provided with optimized buffer system for fast PCR reactions across a range of templates. (*Enzyme only)
10 mL 50,000 Units
MDX001
10 mL
50,000 Units*
100 µL
500 Units
Taq HS DNA Polymerase Antibody-mediated hot-start enzyme ideal for fast multiplex reactions. Robust performance with low-copy number targets even in the presence of PCR inhibitors. (*Enzyme only) Glycerol-Free Taq HS 50 U/µL Lyophilization-compatible, high concentration (50 U/µL), glycerol free DNA enzyme for automated high-throughput testing (provided as separate antibody, enzyme and dilution buffer). Aptamer Taq HS (Glycerol-Free) 50 U/μL A high concentration, lyophilization-compatible Taq DNA polymerase containing a DNA aptamer which binds reversibly to the polymerase. Suitable for developing highly specific, high-throughput assays.
10 mL
50,000 Units
MDX008
10 mL
50,000 Units*
20 µL
1,000 Units
MDX011
500 µL 25,000 Units
20 µL 1,000 Units
MDX015
500 µL
50,000 Units
100 µL
500 Units
Low DNA Taq HS 5 U/µL Heat-activated, thermostable DNA polymerase suited amplification of bacterial and fungal DNA. Low DNA Taq HS 10 U/µL Heat-activated, thermostable DNA polymerase suited to amplification of bacterial and fungal DNA.
MDX009
10 mL
50,000 Units
50 µL
500 Units
MDX010
5 mL
50,000 Units
Human Diagnostics Environmental
Vet Health Water Testing
Blood Banking Food Testing
Genetic Screening DNA Barcoding
4
Polymerases continued
PRODUCT
APPLICATION
CAT NO. VOLUME REACTIONS
High-Fidelity Polymerases High-Fidelity Pfu 3’ - 5’ proofreading exonuclease activity with an error rate of 3.0 x 10 -6 and generates blunt-ended amplicons up to 5 kb in length. High-Specificity Pfu HS Mix High-fidelity, aptamer-based hot start DNA polymerase tailored for low GC bias amplification.
200 µL
500 Units
MDX003
10 mL
25,000 Units
5 mL
200 Rxn
MDX006
100 mL
4,000 Rxn
Isothermal Polymerases
1 mL
-
Bst DNA Polymerase Stable, heat resistant DNA polymerase optimized for LAMP and contains 5´ > 3´ DNA polymerase activity and strong strand displacement activity.
MDX012
20 mL
-
8 µL
-
High Conc. Glycerol-Free Bst Lyophilization-compatible DNA polymerase for isothermal applications.
MDX018
80 µL
-
Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media. Reverse Transcriptase Lyo-compatible MMLV-RT High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays.
2 mL
-
MDX019
100 mL
-
8 µL
1,000 Rxn
MDX042
80 µL
10,000 Rxn
200 µL
1,000 Rxn
RNase-Tolerant MMLV-RT Mixture of MMLV-RT and RNase inhibitor ideal for RT-qPCR assays.
MDX043
2 mL
10,000 Rxn
200 µL
1,000 Rxn
MMLV-RT MMLV-RT suitable for generating first strand cDNA of up to 9 kb.
MDX044
2 mL
10,000 Rxn
Next-Generation Sequencing NGS Clean and Select Beads Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries. NGS ER Enzyme Mix A mix that processes both 3’ and 5’ overhangs generating product that is 5’ phosphorylated with 3’ A overhang.
50 mL
-
MDX041
500 mL
-
6 mL
1,000 Rxn
MDX040
NGS Ligase Enzyme that catalyzes the ligation of adaptors to 3’ A overhangs.
2 mL
1,000 Rxn
MDX037
NGS Library Quantification qPCR-based assay for the quantification of only adapter-ligated library molecules.
1 Kit
500 Rxn
MDX039
Human Diagnostics Environmental
Vet Health Water Testing
Blood Banking Food Testing
Genetic Screening DNA Barcoding
www.MeridianLifeScience.com 5
Components
PRODUCT
APPLICATION
CAT NO. VOLUME REACTIONS
Buffers NGS High-Fidelity Pfu Buffer, 10x Optimized for use with High-Fidelity Pfu (MDX003) .
5 mL
1,000 Rxn
MDX038
NGS End-Repair Buffer, 5x Optimized for use with NGS ER Enzyme Mix (MDX040) .
10 mL
1,000 Rxn
MDX035
NGS Ligase Buffer, 5x Optimized for use with NGS Ligase (MDX037) .
3 mL
1,000 Rxn
MDX036
Taq PCR Buffer, 5x Optimized for use with Taq DNA Polymerase (MDX001) and Taq HS DNA Polymerase (MDX008) . Bst Reaction Buffer, 10x Optimized for use with Bst DNA Polymerase (MDX012) and High Conc. Glycerol-Free Bst (MDX018) .
2 mL
500 Rxn
MDX002
5 mL
-
MDX076
10 mL
-
5 mL
-
Enzyme Dilution Buffer, 1x A glycerol containing 1x dilution buffer, for the dilution of enzymes to reaction concentration.
MDX078
100 mL
-
5 mL
-
Enzyme Dilution Buffer (10x) Glycerol free A glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration. Tissue Extract-PCR Buffers Lysis and neutralization buffer optimized for use with Taq HS DNA Polymerase (MDX008) to perform PCR direct from crude lysate.
MDX080
10 mL
-
1 Kit
1,000 Rxn
MDX004
2.5 mL 500 Rxn 50 mL 10,000 Rxn
Fast qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) .
MDX033
1-Step RT-qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) and RNase-Tolerant MMLV-RT (MDX043) . Lyo-Ready qPCR Buffer, 2.5x Optimized for use with Glycerol-Free Taq HS (MDX011) . In order to produce lyophilized, ambient-temperature stable qPCR reagents, dNTPs, MgCl 2 and glycerol-free Taq DNA polymerase are not included in the buffer.
2.5 mL
500 Rxn
MDX034
50 mL 10,000 Rxn
8 mL
1,000 Rxn
MDX022
100 mL 12,500 Rxn
2.5 mL 500 Rxn 100 mL 20,000 Rxn
Lyo-Ready qPCR Buffer w/o Excipients, 4x Optimized for use with Glycerol-Free Taq HS (MDX011) .
MDX061
Taq Dilution Buffer This Buffer provides the optimal conditions to store Meridian polymerases, conferring long-term stability at -20 °C (MDX008) . Inhibitor-Tolerant PCR Buffer, 5x Used with Taq HS DNA Polymerase (MDX008) for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media. General Reagents Uracil DNA Glycosylase (UDG)* Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free. Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA.
5 mL
-
MDX007
100 mL
-
2 mL
500 Rxn
MDX075
100 mL 20,000 Rxn
2 mL
-
MDX019
100 mL
-
10 mL
10,000 Units
MDX054
25 mL
500 mg
MDX055
* Uracil DNA Glycosylase (MDX054) is not available in the US
6
Components continued
PRODUCT
APPLICATION
CAT NO. VOLUME REACTIONS
General Reagents Continued RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays. Taq HS Antibody A mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity at room temperature. For use in hot-start PCR.
250 µL 10,000 Units
MDX056
2.5 mL 100,000 Units
20 µL
1,000 Units
MDX014
250 µL 12,500 Units
Ready-to-use sequencing grade dNTPs available individually, as sets, or pre-blended dNTP mixes. Ultra-pure (>99% by HPLC) free of PCR inhibitors, DNase and RNase.
dNTPs
PRODUCT
CAT NO. VOLUME
dNTPs Lithium Salt
4 x 250 µL
dNTP Set, 100 mM Provided as four separate solutions at 100 mM concentration (dATP, dCTP, dGTP & dTTP).
MDX050
4 x 50 mL
500 µL
dNTP Mix, 100 mM Supplied as a mixture (dATP, dCTP, dGTP & dTTP). Total concentration of mix is 100 mM (25 mM of each nucleotide).
50 mL
MDX051
100 mL
1 L
dUTP Mix, 50 mM Supplied as a mixture (10 mM dATP, dCTP, dGTP and 20 mM dUTP).
50 mL
MDX058
dUTP, 100 mM
50 mL
MDX059
dATP, 100 mM
50 mL
MDX046
dCTP, 100 mM
50 mL
MDX047
dGTP, 100 mM
50 mL
MDX048
dTTP, 100 mM
50 mL
MDX049
dNTPs Sodium Salt dNTP Mix, 10 mM sodium salt*
Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire
MDX067
dNTP Mix, 40 mM sodium salt*
MDX083
dNTP Mix, 100 mM sodium salt*
MDX084
dATP Mix, 100 mM sodium salt*
MDX063
dCTP Mix, 100 mM sodium salt*
MDX064
dGTP Mix, 100 mM sodium salt*
MDX065
dTTP Mix, 100 mM sodium salt*
MDX066
* Not available in China
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qPCR and RT-qPCR Extraction Controls
The qPCR Extraction Controls are E. coli cells of a known concentration containing the Internal Control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample DNA). Genetic material from the test sample and the DNA extraction control are simultaneously extracted by common extraction methods with the control being as sensitive to the same inhibition and extraction failure as the test sample. Designed for validating the steps within a qPCR or RT-qPCR assay to produce more reliable and accurate results. qPCR
Product Highlights
• Extraction control undergoes the same sample processing as test sample • Can be used with a variety of sample types including inhibitor-rich samples such as blood, urine and sputum
• Confirms successes of extraction step and monitors co-purification of PCR inhibitor inhibitors • Probe based designed specifically for multiplex qPCR assays • Control DNA is unique with no homology to any organism
PRODUCT
CAT NO. VOLUME REACTIONS
qPCR Extraction Control RED (Quasar ® 670)
10 mL
2,000 Rxn
MDX026
qPCR Extraction Control ORANGE (Cal Fluor ® 560)
10 mL
2,000 Rxn
MDX027
VLP-RNA Extraction Control is RNA molecule without any known homology, protected in a protein nanocage, harnessing the stability of viral structures. VLP-RNA Extraction Control is spiked into the biological sample and undergoes the entirety of the sample processing, enabling users of RT-qPCR assay to not only validate the success of the extraction and the reverse transcription steps, but also to determine the presence of PCR inhibitors, reducing the chance of obtaining false negatives and resulting in reliable and accurate results. The VLP-RNA Extraction Control is provided with its own detection system and can have its sequence customized if necessary. RT-qPCR
Product Highlights
• Contains a defined number of copies of target RNA molecules, encapsidated within a virus-like particle (VLP) • RNA sequence is customizable up to 1000nt • Non-infectious material for ease of handling and shipping
• Closely mimics the test sample, undergoing the same processing route from lysis and extraction to RT-qPCR detection • Compatible with commonly used RNA extraction methods and lyophilization
PRODUCT
CAT NO.
REACTIONS
1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL) 1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL)
VLP-RNA Extraction Control Red
MDX068
VLP-RNA Extraction Control Orange
MDX069
VLP-RNA Extraction Control
Please enquire
MDX071
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com
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