Reagent Solutions for Molecular Diagnostics

Reagent Solutions for Molecular Diagnostics PCR/RT-PCR Enzymes, Optimized Mixes and dNTPs

MOLECULAR REAGENTS FOR MDX APPLICATIONS

BLOOD BANKING

HUMAN DIAGNOSTICS

VET HEALTH

GENETIC SCREENING

FOOD TESTING

ENVIRONMENTAL

DNA BARCODING

WATER TESTING

Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety. Products include PCR and RT-PCR enzymes, ultra-pure dNTPs and buffer systems for IVD assay development and optimization. In addition to these reagents, Meridian has also developed a range of ready-to-use solutions for molecular diagnostic manufacturers. These include enzymes and mixes formulated for lyophilization, internal quality controls to monitor extraction and co-purification of inhibitor in qPCR/RT-qPCR assays as well as a unique mix designed for direct qPCR from crude lysates.

www.MeridianLifeScience.com

Product Selection Chart

• Suitable •• Recommended

      

••

••

••

Low DNA qPCR Mix

MDX030

Chemical

•• ••

••

Fast qPCR Mix

MDX020

Antibody

MDX031 & MDX060

• ••

Hi-Throughput dUTP Mix

Antibody

•• ••

Inhibitor-Tolerant qPCR Mix

MDX013

Antibody

•• •• •• •• ••

Inhibitor-Tolerant RT-qPCR Mix

MDX016

Antibody

•• ••

Fast 1-Step RT-qPCR Mix

MDX032

Antibody

•• •• ••

Low LOD 1-Step RT-qPCR Mix

MDX025

Antibody

••

• •

Lyo-Compatible MMLV-RT

MDX042

No

• ••

RNase-Tolerant MMLV-RT

MDX043

No

• •

MMLV-RT

MDX044

No

     

••

•• ••

Air-Dryable ™ qPCR Mix

MDX082

Antibody

••

•• •• • •

Air-Dryable ™ 1-Step RT-qPCR Mix

MDX095

Antibody

••

•• •• •• •

Air-Dryable ™ Direct DNA qPCR Blood

MDX092

Antibody

MDX021 & MDX023

••

•• ••

Lyo-Ready ™ qPCR Mix

Antibody

••

• ••

Lyo-Ready ™ 1-Step RT-qPCR Mix

MDX024

Antibody

••

• ••

Lyo-Ready ™ 1-Step RT-qPCR Virus Mix

MDX062

Antibody

••

••

•• ••

Lyo-Ready ™ LAMP Mix

MDX097

No

••

••

Bst DNA Polymerase

MDX012

No

••

••

High Conc. Glycerol-Free Bst

MDX018

No

• • •

Taq DNA Polymerase

MDX001

No

•• •• •• ••

Taq HS DNA Polymerase

MDX008

Antibody

••

•• •

Glycerol-Free Taq HS

MDX011

Antibody

••

•• ••

••

Aptamer Taq HS (Glycerol-Free)

MDX015

Aptamer

MDX009 & MDX010

••

••

Low DNA Taq HS

Chemical

••

High-Fidelity Pfu

MDX003

No

••

•• ••

High-Specificity Pfu HS

MDX006

Aptamer

2

Optimized Mixes

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

qPCR (DNA Template) Hot-Start Master Mixes Low DNA qPCR Mix Ideal for microbial and fungal DNA detection assays.

5 mL

500 Rxn

MDX030

100 mL

10,000 Rxn

5 mL

500 Rxn

Fast qPCR Mix Suitable for fast high-multiplex qPCR assays.

MDX020

100 mL

10,000 Rxn

2 mL

500 Rxn

Fast qPCR Mix, 5x Suitable for fast high-multiplex qPCR assays (5x concentration).

MDX072

40 mL

10,000 Rxn

2 mL

500 Rxn

Digital PCR Mix, 5x Suitable for digital PCR (dPCR) assays (5x concentration).

MDX074

40 mL

10,000 Rxn

Hi-throughput dUTP qPCR Mix Optimized formulation for efficient dUTP incorporation to remove background PCR product contamination. Inhibitor-Tolerant qPCR Mix Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant qPCR Mix, 5x Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants (5x concentration). RT-qPCR (RNA Template) Hot-Start Master Mixes Fast 1-Step RT-qPCR Mix RT-qPCR mix formulated for fast, automated, high-throughput systems. Inhibitor-Tolerant RT-qPCR Mix, 4x All-in-one, 4x master mix designed for RT-qPCR from crude samples such as sputum, stool and saliva. No sample extraction required. Low LOD 1-Step RT-qPCR Mix Suitable for detecting RNA and DNA viruses at very low levels for applications such as blood bank or transplant viral testing.

5 mL 500 Rxn 100 mL 10,000 Rxn 500 Rxn 100 mL 10,000 Rxn 5 mL

MDX031

MDX013

2 mL

500 Rxn

MDX073

40 mL

10,000 Rxn

10 mL 1,000 Rxn 100 mL 10,000 Rxn 200 Rxn 50 mL 10,000 Rxn 1 mL 10 mL 1,000 Rxn 100 mL 10,000 Rxn

MDX032

MDX016

MDX025

Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes Air-Dryable ™ qPCR Mix, 4x Ready-to-use qPCR mix ideal for oven or air-drying technologies.

5 mL 1,000 Rxn 50 mL 10,000 Rxn

MDX082

5 mL

1,000 Rxn

Air-Dryable ™ RT-qPCR Mix, 4x Ready-to-use RT-qPCR Mix ideal for oven or air drying technologies. Air-Dryable ™ Direct DNA qPCR Blood, 4x Inhibitor-tolerant qPCR mix ideal for oven or air-drying technologies. Can be used for direct detection using whole blood, serum or plasma samples. Lyo-Ready ™ qPCR Mix Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.

MDX095

50 mL

10,000 Rxn

5 mL

1,000 Rxn

MDX092

50 mL

10,000 Rxn

5 mL

500 Rxn

MDX021

100 mL

10,000 Rxn

Human Diagnostics Environmental

Vet Health Water Testing

Blood Banking Food Testing

Genetic Screening DNA Barcoding

www.MeridianLifeScience.com 3

Optimized Mixes continued

PRODUCT

APPLICATION

CAT NO. VOLUME REACTIONS

Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes Continued Lyo-Ready ™ qPCR Mix, 2.6x

8 mL

1,000 Rxn

MDX023

Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets (2.6x concentration). Lyo-Ready ™ 1-Step RT-qPCR Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ 1-Step RT-qPCR Virus Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients and highly suited for amplification of RNA viruses with a high secondary structure. Loop-Mediated Isothermal Amplification (LAMP) Master Mixes Lyo-ready ™ LAMP Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.

100 mL 12,500 Rxn

10 mL

1,000 Rxn

MDX024

100 mL

10,000 Rxn

10 mL

1,000 Rxn

MDX062

100 mL

10,000 Rxn

5 mL

800 Rxn

MDX097

50 mL

8,000 Rxn

Polymerases

PRODUCT

APPLICATION

CAT NO. VOLUME REACTIONS

DNA Polymerases

100 µL

500 Units

Taq DNA Polymerase DNA Polymerase provided with optimized buffer system for fast PCR reactions across a range of templates. (*Enzyme only)

10 mL 50,000 Units

MDX001

10 mL

50,000 Units*

100 µL

500 Units

Taq HS DNA Polymerase Antibody-mediated hot-start enzyme ideal for fast multiplex reactions. Robust performance with low-copy number targets even in the presence of PCR inhibitors. (*Enzyme only) Glycerol-Free Taq HS 50 U/µL Lyophilization-compatible, high concentration (50 U/µL), glycerol free DNA enzyme for automated high-throughput testing (provided as separate antibody, enzyme and dilution buffer). Aptamer Taq HS (Glycerol-Free) 50 U/μL A high concentration, lyophilization-compatible Taq DNA polymerase containing a DNA aptamer which binds reversibly to the polymerase. Suitable for developing highly specific, high-throughput assays.

10 mL

50,000 Units

MDX008

10 mL

50,000 Units*

20 µL

1,000 Units

MDX011

500 µL 25,000 Units

20 µL 1,000 Units

MDX015

500 µL

50,000 Units

100 µL

500 Units

Low DNA Taq HS 5 U/µL Heat-activated, thermostable DNA polymerase suited amplification of bacterial and fungal DNA. Low DNA Taq HS 10 U/µL Heat-activated, thermostable DNA polymerase suited to amplification of bacterial and fungal DNA.

MDX009

10 mL

50,000 Units

50 µL

500 Units

MDX010

5 mL

50,000 Units

Human Diagnostics Environmental

Vet Health Water Testing

Blood Banking Food Testing

Genetic Screening DNA Barcoding

4

Polymerases continued

PRODUCT

APPLICATION

CAT NO. VOLUME REACTIONS

High-Fidelity Polymerases High-Fidelity Pfu 3’ - 5’ proofreading exonuclease activity with an error rate of 3.0 x 10 -6 and generates blunt-ended amplicons up to 5 kb in length. High-Specificity Pfu HS Mix High-fidelity, aptamer-based hot start DNA polymerase tailored for low GC bias amplification.

200 µL

500 Units

MDX003

10 mL

25,000 Units

5 mL

200 Rxn

MDX006

100 mL

4,000 Rxn

Isothermal Polymerases

1 mL

-

Bst DNA Polymerase Stable, heat resistant DNA polymerase optimized for LAMP and contains 5´ > 3´ DNA polymerase activity and strong strand displacement activity.

MDX012

20 mL

-

8 µL

-

High Conc. Glycerol-Free Bst Lyophilization-compatible DNA polymerase for isothermal applications.

MDX018

80 µL

-

Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media. Reverse Transcriptase Lyo-compatible MMLV-RT High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays.

2 mL

-

MDX019

100 mL

-

8 µL

1,000 Rxn

MDX042

80 µL

10,000 Rxn

200 µL

1,000 Rxn

RNase-Tolerant MMLV-RT Mixture of MMLV-RT and RNase inhibitor ideal for RT-qPCR assays.

MDX043

2 mL

10,000 Rxn

200 µL

1,000 Rxn

MMLV-RT MMLV-RT suitable for generating first strand cDNA of up to 9 kb.

MDX044

2 mL

10,000 Rxn

Next-Generation Sequencing NGS Clean and Select Beads Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries. NGS ER Enzyme Mix A mix that processes both 3’ and 5’ overhangs generating product that is 5’ phosphorylated with 3’ A overhang.

50 mL

-

MDX041

500 mL

-

6 mL

1,000 Rxn

MDX040

NGS Ligase Enzyme that catalyzes the ligation of adaptors to 3’ A overhangs.

2 mL

1,000 Rxn

MDX037

NGS Library Quantification qPCR-based assay for the quantification of only adapter-ligated library molecules.

1 Kit

500 Rxn

MDX039

Human Diagnostics Environmental

Vet Health Water Testing

Blood Banking Food Testing

Genetic Screening DNA Barcoding

www.MeridianLifeScience.com 5

Components

PRODUCT

APPLICATION

CAT NO. VOLUME REACTIONS

Buffers NGS High-Fidelity Pfu Buffer, 10x Optimized for use with High-Fidelity Pfu (MDX003) .

5 mL

1,000 Rxn

MDX038

NGS End-Repair Buffer, 5x Optimized for use with NGS ER Enzyme Mix (MDX040) .

10 mL

1,000 Rxn

MDX035

NGS Ligase Buffer, 5x Optimized for use with NGS Ligase (MDX037) .

3 mL

1,000 Rxn

MDX036

Taq PCR Buffer, 5x Optimized for use with Taq DNA Polymerase (MDX001) and Taq HS DNA Polymerase (MDX008) . Bst Reaction Buffer, 10x Optimized for use with Bst DNA Polymerase (MDX012) and High Conc. Glycerol-Free Bst (MDX018) .

2 mL

500 Rxn

MDX002

5 mL

-

MDX076

10 mL

-

5 mL

-

Enzyme Dilution Buffer, 1x A glycerol containing 1x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX078

100 mL

-

5 mL

-

Enzyme Dilution Buffer (10x) Glycerol free A glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration. Tissue Extract-PCR Buffers Lysis and neutralization buffer optimized for use with Taq HS DNA Polymerase (MDX008) to perform PCR direct from crude lysate.

MDX080

10 mL

-

1 Kit

1,000 Rxn

MDX004

2.5 mL 500 Rxn 50 mL 10,000 Rxn

Fast qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) .

MDX033

1-Step RT-qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) and RNase-Tolerant MMLV-RT (MDX043) . Lyo-Ready qPCR Buffer, 2.5x Optimized for use with Glycerol-Free Taq HS (MDX011) . In order to produce lyophilized, ambient-temperature stable qPCR reagents, dNTPs, MgCl 2 and glycerol-free Taq DNA polymerase are not included in the buffer.

2.5 mL

500 Rxn

MDX034

50 mL 10,000 Rxn

8 mL

1,000 Rxn

MDX022

100 mL 12,500 Rxn

2.5 mL 500 Rxn 100 mL 20,000 Rxn

Lyo-Ready qPCR Buffer w/o Excipients, 4x Optimized for use with Glycerol-Free Taq HS (MDX011) .

MDX061

Taq Dilution Buffer This Buffer provides the optimal conditions to store Meridian polymerases, conferring long-term stability at -20 °C (MDX008) . Inhibitor-Tolerant PCR Buffer, 5x Used with Taq HS DNA Polymerase (MDX008) for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media. General Reagents Uracil DNA Glycosylase (UDG)* Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free. Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA.

5 mL

-

MDX007

100 mL

-

2 mL

500 Rxn

MDX075

100 mL 20,000 Rxn

2 mL

-

MDX019

100 mL

-

10 mL

10,000 Units

MDX054

25 mL

500 mg

MDX055

* Uracil DNA Glycosylase (MDX054) is not available in the US

6

Components continued

PRODUCT

APPLICATION

CAT NO. VOLUME REACTIONS

General Reagents Continued RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays. Taq HS Antibody A mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity at room temperature. For use in hot-start PCR.

250 µL 10,000 Units

MDX056

2.5 mL 100,000 Units

20 µL

1,000 Units

MDX014

250 µL 12,500 Units

Ready-to-use sequencing grade dNTPs available individually, as sets, or pre-blended dNTP mixes. Ultra-pure (>99% by HPLC) free of PCR inhibitors, DNase and RNase.

dNTPs

PRODUCT

CAT NO. VOLUME

dNTPs Lithium Salt

4 x 250 µL

dNTP Set, 100 mM Provided as four separate solutions at 100 mM concentration (dATP, dCTP, dGTP & dTTP).

MDX050

4 x 50 mL

500 µL

dNTP Mix, 100 mM Supplied as a mixture (dATP, dCTP, dGTP & dTTP). Total concentration of mix is 100 mM (25 mM of each nucleotide).

50 mL

MDX051

100 mL

1 L

dUTP Mix, 50 mM Supplied as a mixture (10 mM dATP, dCTP, dGTP and 20 mM dUTP).

50 mL

MDX058

dUTP, 100 mM

50 mL

MDX059

dATP, 100 mM

50 mL

MDX046

dCTP, 100 mM

50 mL

MDX047

dGTP, 100 mM

50 mL

MDX048

dTTP, 100 mM

50 mL

MDX049

dNTPs Sodium Salt dNTP Mix, 10 mM sodium salt*

Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire

MDX067

dNTP Mix, 40 mM sodium salt*

MDX083

dNTP Mix, 100 mM sodium salt*

MDX084

dATP Mix, 100 mM sodium salt*

MDX063

dCTP Mix, 100 mM sodium salt*

MDX064

dGTP Mix, 100 mM sodium salt*

MDX065

dTTP Mix, 100 mM sodium salt*

MDX066

* Not available in China

www.MeridianLifeScience.com 7

qPCR and RT-qPCR Extraction Controls

The qPCR Extraction Controls are E. coli cells of a known concentration containing the Internal Control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample DNA). Genetic material from the test sample and the DNA extraction control are simultaneously extracted by common extraction methods with the control being as sensitive to the same inhibition and extraction failure as the test sample. Designed for validating the steps within a qPCR or RT-qPCR assay to produce more reliable and accurate results. qPCR

Product Highlights

• Extraction control undergoes the same sample processing as test sample • Can be used with a variety of sample types including inhibitor-rich samples such as blood, urine and sputum

• Confirms successes of extraction step and monitors co-purification of PCR inhibitor inhibitors • Probe based designed specifically for multiplex qPCR assays • Control DNA is unique with no homology to any organism

PRODUCT

CAT NO. VOLUME REACTIONS

qPCR Extraction Control RED (Quasar ® 670)

10 mL

2,000 Rxn

MDX026

qPCR Extraction Control ORANGE (Cal Fluor ® 560)

10 mL

2,000 Rxn

MDX027

VLP-RNA Extraction Control is RNA molecule without any known homology, protected in a protein nanocage, harnessing the stability of viral structures. VLP-RNA Extraction Control is spiked into the biological sample and undergoes the entirety of the sample processing, enabling users of RT-qPCR assay to not only validate the success of the extraction and the reverse transcription steps, but also to determine the presence of PCR inhibitors, reducing the chance of obtaining false negatives and resulting in reliable and accurate results. The VLP-RNA Extraction Control is provided with its own detection system and can have its sequence customized if necessary. RT-qPCR

Product Highlights

• Contains a defined number of copies of target RNA molecules, encapsidated within a virus-like particle (VLP) • RNA sequence is customizable up to 1000nt • Non-infectious material for ease of handling and shipping

• Closely mimics the test sample, undergoing the same processing route from lysis and extraction to RT-qPCR detection • Compatible with commonly used RNA extraction methods and lyophilization

PRODUCT

CAT NO.

REACTIONS

1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL) 1 mL (~1x10 4 copies/µL) 20 mL (~1x10 4 copies/µL)

VLP-RNA Extraction Control Red

MDX068

VLP-RNA Extraction Control Orange

MDX069

VLP-RNA Extraction Control

Please enquire

MDX071

Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299

Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com

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