Reagent Solutions for Molecular Diagnostics

Enzymes, Mixes & Other Molecular Reagents. Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety.

PCR/LAMP Enzymes, Optimized Mixes and dNTPs

Specimen-specific™Master Mixes Ready-to-use master mixes designed for direct qPCR or LAMP from a variety of clinical specimens such as blood, saliva, urine and stool. Mixes can also be used with purified templates to minimize the effects of potential contaminants or carry-over. Lyo-Ready™and Air-Dryable™Options Creating an ambient-temperature stable assay has never been easier. Lyo-Ready™ and Air-Dryable™ mixes are highly concentrated (typically 4x), glycerol-free, and contain an optimized blend of excipients for lyophilization or air-drying.

Point-of-care Testing: Glycerol-free Enzymes

PCR, Isothermal & NGS Enzymes Various formulations of Taq (aptamer hot-start, etc.), Pfu, MMLV-RT, Bst and enzymes for NGS library preparation designed with specialized buffers to create your own master mix Broad range of highly purified enzymes that are free from glycerol. These enzymes are specially designed to be compatible with lyophilization or air-drying, allowing for easy integration into ambient- temperature point-of-care platforms.

Internal qPCR Controls

dNTPs & General Reagents

Internal controls are designed to confirm the success of the extraction step and reduce the chance of obtaining a false negative result. Innovative VLP- Extraction Controls allow you to monitor extraction, reverse transcription and amplification using a single control. The sequences can be customized to meet the design of a specific assay.

A one-stop solution for sodium and lithium dNTPs salts (available as mixes or individual components), UDG, proteinase K, RNase inhibitor and other assay components such as Taq hot-start antibody.

Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety. Products include PCR/RT-PCR and LAMP enzymes, ultra-pure dNTPs and buffer systems for IVD assay development and optimization. In addition to these reagents, Meridian has also developed a range of ready-to-use solutions for molecular diagnostic manufacturers. These include enzymes and mixes formulated for lyophilization or air-drying, internal quality controls to monitor extraction and co-purification of inhibitor in qPCR/RT-qPCR assays, as well as a unique mix designed for direct qPCR from a variety of clinical specimens (e.g. blood, saliva, urine and stool).

www.MeridianBioscience.com/lifescience

Product Selection Chart • Recommended

For Air- Drying

Direct from samples

2X Master Mix

4X Master Mix

For Purified DNA/RNA

Proof- Reading

For Lyophilization

Hot-Start

Cat#

• • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • •

Taq DNA Polymerase

MDX001

Taq HS DNA Polymerase

MDX008

Antibody

•

Glycerol-Free Taq HS 50 U/µL

MDX011

Antibody

Low DNA Taq HS 5 U/µL

MDX009

Chemical

Aptamer Taq HS (Glycerol-Free)

MDX015

Aptamer

• •

High-Fidelity Pfu

MDX003

Antibody

•

Glycerol-Free High-Fidelity Pfu

MDX203

Antibody

Bst DNA Polymerase

MDX012

Antibody

• •

High Conc. Glycerol-Free Bst (100 U/μL)

MDX018

Lyo-Compatible MMLV-RT

MDX042

•

RNase-Tolerant MMLV-RT

MDX043

MMLV-RT

MDX044

• • • •

• •

55C MMLV-RT

MDX117

Glycerol-Free AMV Reverse Transcriptase (HC)

MDX209

Glycerol-Free T4 DNA Ligase (HC)

MDX200

Glycerol-Free T7 RNA Polymerase (HC)

MDX201

•

High-Specificity Pfu HS Mix

MDX006

Aptamer

Digital PCR Mix, 5x

MDX072

Antibody

• • • • • •

Fast qPCR Mix

MDX020

Antibody

Fast 1-Step RT-qPCR Mix

MDX032

Antibody

Hi-Throughput dUTP qPCR Mix

MDX031

Antibody

Low DNA qPCR Mix

MDX030

Chemical

Low LOD 1-Step RT-qPCR Mix

MDX025

Antibody

• • • • • • • • • •

Inhibitor-Tolerant qPCR Mix

MDX013

Antibody

•

Inhibitor-Tolerant RT-qPCR Mix

MDX016

Antibody

Inhibitor-Tolerant qPCR Mix, 5x

MDX073

Antibody

• • •

• • • • • • •

Lyo-Ready™ qPCR Mix

MDX021

Antibody

Lyo-Ready™ 1-Step RT-qPCR Mix

MDX024

Antibody

Lyo-Ready™ 1-Step RT-qPCR Virus Mix

MDX062

Antibody

MDX122 & MDX123 MDX132 & MDX133 MDX142 & MDX143 MDX152 & MDX153

• • • •

Lyo-Ready™ Direct DNA and RNA/DNA qPCR Blood Mixes

Antibody

Lyo-Ready™ Direct DNA and RNA/DNA qPCR Saliva Mixes

Lyo-Ready™ Direct DNA and RNA/DNA qPCR Stool Mixes

Antibody

Lyo-Ready™ Direct DNA and RNA/DNA qPCR Urine Mixes

Antibody

Product Selection Chart • Recommended

For Air- Drying

Direct from samples

2X Master Mix

4X Master Mix

For Purified DNA/RNA

Proof- Reading

For Lyophilization

Hot-Start

Cat#

• • • • • • • • • •

• • • •

• • • • • • • • • •

• • • • • • • • • • • • • • • • • • • • • • • •

Lyo-Ready™ Genotyping Direct qPCR Blood Mix

MDX128

Antibody

Lyo-Ready™ Genotyping Direct qPCR Stool Mix

MDX148

Antibody

Lyo-Ready™ Genotyping Direct qPCR Urine Mix

MDX158

Antibody

Lyo-Ready™ Genotyping Direct qPCR FFPE

MDX168

Antibody

MDX082 & MDX095 MDX092 & MDX121 MDX130 & MDX131 MDX140 & MDX141 MDX150 & MDX151

• • • • • •

Air-Dryable™ qPCR Mix and RT-qPCR Mixes

Antibody

Air-Dryable™ Direct DNA and RNA/DNA qPCR Blood Mixes

Antibody

Air-Dryable™ Direct DNA and RNA/DNA qPCR Saliva Mixes

Antibody

Air-Dryable™ Direct DNA and RNA/DNA qPCR Stool Mixes

Antibody

Air-Dryable™ Direct DNA and RNA/DNA qPCR Urine Mixes

Air-Dryable™ Direct DNA qPCR Plant Mix

MDX116

Antibody

Bst DNA Polymerase

MDX012

•

High Conc. Glycerol-Free Bst

MDX018

MDX118 & MDX119 MDX097 & MDX108 MDX124 & MDX125 MDX134 & MDX135 MDX144 & MDX145 MDX154 & MDX155

• • • • • •

•

• • • • • •

Air-Dryable™ LAMP and RT-LAMP Mix

• • • • •

Lyo-Ready™ LAMP Mix and RT-LAMP Mix

Lyo-Ready™ Direct DNA and RNA/DNA LAMP Blood Mixes

Lyo-Ready™ Direct DNA and RNA/DNA LAMP Saliva Mixes

Lyo-Ready™ Direct DNA and RNA/DNA LAMP Stool Mixes

Lyo-Ready™ Direct DNA and RNA/DNA LAMP Urine Mixes

• •

Glycerol-Free T4 DNA Ligase (HC)

MDX200

• • •

Glycerol-Free High-Fidelity Pfu

MDX203

High-Fidelity Pfu

MDX003

Antibody

•

High-Specificity Pfu HS Mix

MDX006

Aptamer

NGS Clean and Select Beads

MDX041

NGS Library Quantification

MDX039

Primers and probes supplied

Extraction & amplification Extraction & amplification Extraction, reverse transcription & amplification Extraction, reverse transcription & amplification Used to determine

Lyo- Compatible

Custom sequence

Fluorescence dye

DNA

RNA

Cat#

• •

• Recommended qPCR Extraction Control Red qPCR Extraction Control Orange

MDX026

Quasar® 670

MDX027

Cal Fluor® 560

•

VLP-RNA Extraction Control Red

MDX068

Cy5

•

VLP-RNA Extraction Control Orange

MDX069

HEX

www.MeridianBioscience.com/lifescience 3

Optimized Master Mixes

Inhibitor-Tolerant qPCR/RT-qPCR

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

UNIVERSAL Inhibitor-Tolerant qPCR Mix Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant RT-qPCR Mix, 4x All-in-one, 4x master mix designed for RT-qPCR from crude samples such as sputum, stool and saliva. No sample extraction required.

5 mL

500 Rxns

MDX013

100 mL 10,000 Rxns

1 mL

200 Rxns

MDX016

50 mL 10,000 Rxns

BLOOD, PLASMA, SERUM Air-Dryable ™ Direct DNA and RNA/DNA qPCR Blood, 4x Inhibitor-tolerant qPCR and RT-qPCR mixes ideal for oven or air-drying technologies. Can be used for direct detection using whole blood, serum or plasma samples. Lyo-Ready ™ Direct DNA and RNA/DNA qPCR Blood, 4x Ready-to-use, glycerol-free qPCR and RT-qPCR mixes formulated with a specialized blend of excipients and highly suited for direct detection using whole blood, serum or plasma samples. Lyo-Ready ™ Genotyping Direct qPCR Blood, 4x Glycerol-free, one-tube formulation optimized for SNP genotyping applications with dual-label probes. Formulated for overcoming inhibitors found in blood samples and can be used with direct amplification protocols on blood, serum or plasma samples. SALIVA, SPUTUM Air-Dryable ™ Direct DNA and RNA/DNA qPCR Saliva, 4x Inhibitor-tolerant qPCR and RT-qPCR mixes ideal for oven or air-drying technologies. Can be used for direct detection using saliva or sputum samples.

5 mL 1,000 Rxns

MDX092 & MDX121

50 mL

10,000 Rxns

5 mL 1,000 Rxns

MDX122 & MDX123

50 mL

10,000 Rxns

5 mL 1,000 Rxns

MDX128

50 mL

10,000 Rxns

5 mL 1,000 Rxns

MDX130 & MDX131

50 mL

10,000 Rxns

2 mL

500 Rxns

Inhibitor-Tolerant qPCR Mix, 5x Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants (5x concentration).

MDX073

40 mL

10,000 Rxns

5 mL 1,000 Rxns

Lyo-Ready ™ Direct DNA and RNA/DNA qPCR Saliva, 4x Ready-to-use, glycerol-free qPCR and RT-qPCR mixes formulated with a specialized blend of excipients and highly suited for direct detection using saliva and sputum samples. URINE Air-Dryable ™ Direct DNA and RNA/DNA qPCR Urine, 4x Inhibitor-tolerant qPCR and RT-qPCR mixes ideal for oven or air-drying technologies. Can be used for direct detection using urine samples. Lyo-Ready ™ Direct DNA and RNA/DNA qPCR Urine, 4x Ready-to-use, glycerol-free qPCR and RT-qPCR mixes formulated with a specialized blend of excipients and highly suited for direct detection using urine samples.. Lyo-Ready™ Genotyping Direct qPCR Urine, 4x Glycerol-free, one-tube formulation optimized for SNP genotyping applications with dual-label probes. Formulated for overcoming inhibitors found in urine and can be used with direct amplification protocols on urine samples. STOOL Air-Dryable ™ Direct DNA and RNA/DNA qPCR Stool, 4x Inhibitor-tolerant qPCR and RT-qPCR mixes ideal for oven or air-drying technologies. Can be used for direct detection using stool samples. Lyo-Ready ™ Direct DNA and RNA/DNA qPCR Stool, 4x Ready-to-use, glycerol-free qPCR and RT-qPCR mixes formulated with a specialized blend of excipients and highly suited for direct detection using stool samples.

MDX132 & MDX133

50 mL 10,000 Rxns

5 mL 1,000 Rxns

MDX150 & MDX151

50 mL 10,000 Rxns

5 mL

1,000 Rxns

MDX152 & MDX153

50 mL 10,000 Rxns

5 mL

1,000 Rxns

MDX158

50 mL 10,000 Rxns

5 mL 1,000 Rxns

MDX140 & MDX141

50 mL

10,000 Rxns

5 mL 1,000 Rxns

MDX142 & MDX143

50 mL

10,000 Rxns

Optimized Master Mixes continued

Inhibitor-Tolerant qPCR/RT-qPCR continued

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

STOOL continued Lyo-Ready™ Genotyping Direct qPCR Stool, 4x

5 mL 1,000 Rxns

Glycerol-free, one-tube formulation optimized for SNP genotyping applications with dual-label probes. Formulated for overcoming inhibitors found in stool and can be used with direct amplification protocols on crude stool samples.

MDX148

50 mL

10,000 Rxns

PLANT

5 mL 1,000 Rxns

Air-Dryable ™ Direct DNA qPCR Plant Mix Inhibitor-tolerant qPCR mix designed for multiplex amplification of DNA targets from plants.

MDX116

50 mL

10,000 Rxns

FFPE Lyo-Ready™ Genotyping Direct qPCR FFPE,4x Optimised for highly specific allelic discrimination with excellent cluster separation from FFPE tissue, even after lyophilization, ideal for high throughput screening.

5 mL 1,000 Rxns

MDX168

50 mL

10,000 Rxns

Ambient-Temperature Stable qPCR/RT-qPCR Hot-Start Master Mixes MIXES CAN BE USED AS A LIQUID OR AIR-DRIED TO CREATE AMBIENT-TEMPERATURE STABLE MIXES Air-Dryable ™ qPCR Mix, 4x Inhibitor-tolerant qPCR mix designed for multiplex amplification of DNA targets from a wide range of clinical specimens.

5 mL 1,000 Rxns

MDX082

50 mL 10,000 Rxns

5 mL 1,000 Rxns

Air-Dryable ™ RT-qPCR Mix, 4x Inhibitor-tolerant RT-qPCR mix designed for multiplex amplification of DNA targets from a wide range of clinical specimens.

MDX095

50 mL

10,000 Rxns

MIXES CAN BE USED AS A LIQUID OR LYOPHILIZED TO CREATE AMBIENT-TEMPERATURE STABLE MIXES Lyo-Ready ™ qPCR Mix Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.

5 mL

500 Rxns

MDX021

100 mL

10,000 Rxns

8 mL 1,000 Rxns

Lyo-Ready ™ qPCR Mix, 2.6x Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets (2.6x concentration). Lyo-Ready ™ 1-Step RT-qPCR Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ 1-Step RT-qPCR Virus Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients and highly suited for amplification of RNA viruses with a high secondary structure.

MDX023

100 mL 12,500 Rxns

10 mL 1,000 Rxns

MDX024

100 mL

10,000 Rxns

10 mL 1,000 Rxns

MDX062

100 mL

10,000 Rxns

www.MeridianBioscience.com/lifescience 5

Optimized Master Mixes continued

LAMP Master Mixes

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

UNIVERSAL Air-Dryable ™ LAMP and RT-LAMP Mixes, 4X Ready-to-use, glycerol-free LAMP and RT-LAMP master mixes ideal for oven or air-drying technologies. Lyo-Ready ™ LAMP Mix Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. Lyo-Ready ™ RT-LAMP Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.

5 mL

800 Rxns

MDX119 & MDX118

50 mL

8,000 Rxns

5 mL

800 Rxns

MDX097

50 mL

8,000 Rxns

5 mL

800 Rxns

MDX108

50 mL 8,000 Rxns

BLOOD, PLASMA, SERUM Lyo-Ready ™ Direct DNA and RNA/DNA LAMP Blood, 4x

5 mL

800 Rxns

MDX124 & MDX125

Ready-to-use, glycerol-free LAMP master mixes formulated with a specialized blend of excipients for lyophilization. Suited for direct detection using whole blood, serum or plasma samples.

50 mL

8,000 Rxns

SALIVA, SPUTUM Lyo-Ready ™ Direct DNA and RNA/DNA LAMP Saliva, 4x Ready-to-use, glycerol-free LAMP master mixes formulated with a specialized blend of excipients for lyophilization. Suited for direct detection using saliva and sputum samples. STOOL Lyo-Ready ™ Direct DNA and RNA/DNA LAMP Stool, 4x Ready-to-use, glycerol-free LAMP master mixes formulated with a specialized blend of excipients for lyophilization. Suited for direct detection using stool samples. URINE Lyo-Ready ™ Direct DNA and RNA/DNA LAMP Urine, 4x Ready-to-use, glycerol-free LAMP master mixes formulated with a specialized blend of excipients for lyophilization. Suited for direct detection using urine samples.

5 mL

800 Rxns

MDX134 & MDX135

50 mL

8,000 Rxns

5 mL

800 Rxns

MDX144 & MDX145

50 mL

8,000 Rxns

5 mL

800 Rxns

MDX154 & MDX155

50 mL

8,000 Rxns

Optimized Master Mixes continued

qPCR (DNA Template) Hot-Start Master Mixes

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

5 mL

500 Rxns

Low DNA qPCR Mix Ideal for microbial and fungal DNA detection assays.

MDX030

100 mL

10,000 Rxns

5 mL

500 Rxns

Fast qPCR Mix Suitable for fast high-multiplex qPCR assays.

MDX020

100 mL

10,000 Rxns

2 mL

500 Rxns

Fast qPCR Mix, 5x Suitable for fast high-multiplex qPCR assays (5x concentration).

MDX072

40 mL

10,000 Rxns

2 mL

500 Rxns

Digital PCR Mix, 5x Suitable for digital PCR (dPCR) assays (5x concentration).

MDX074

40 mL

10,000 Rxns

5 mL

500 Rxns

Hi-throughput dUTP qPCR Mix Optimized formulation for efficient dUTP incorporation to remove background PCR product contamination.

MDX031

100 mL 10,000 Rxns

10 mL 1000 Rxns

Hi-throughput dUTP qPCR Lo-ROX Optimized formulation with ROX dye for efficient dUTP incorporation to deliver very low background, allowing greater sensitivity.

MDX060

100 mL 10,000 Rxns

RT-qPCR (RNA Template) Hot-Start Master Mixes

10 mL 1,000 Rxns

Fast 1-Step RT-qPCR Mix RT-qPCR mix formulated for fast, automated, high-throughput systems.

MDX032

100 mL 10,000 Rxns

Low LOD 1-Step RT-qPCR Mix Suitable for detecting RNA and DNA viruses at very low levels for applications such as blood bank or transplant viral testing.

10 mL

1,000 Rxns

MDX025

100 mL 10,000 Rxns

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Polymerase

DNA Polymerases

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

100 µL 500 Units 10 mL 50,000 Units 10 mL 50,000 Units* 100 µL 500 Units 10 mL 50,000 Units 10 mL 50,000 Units*

Taq DNA Polymerase DNA Polymerase provided with optimized buffer system for fast PCR reactions across a range of templates. (*Enzyme only)

MDX001

Taq HS DNA Polymerase Antibody-mediated hot-start enzyme ideal for fast multiplex reactions. Robust performance with low-copy number targets even in the presence of PCR inhibitors. (*Enzyme only) Glycerol-Free Taq HS 50 U/µL Lyophilization-compatible, high concentration (50 U/µL), glycerol free DNA enzyme for automated high-throughput testing (provided as separate antibody, enzyme and dilution). Aptamer Taq HS (Glycerol-Free) 50 U/μL A high concentration, lyophilization-compatible Taq DNA polymerase containing a DNA aptamer which binds reversibly to the polymerase. Suitable for developing highly specific, high-throughput assays.

MDX008

20 µL 1,000 Units

MDX011

500 µL 25,000 Units

20 µL 1,000 Units

MDX015

500 µL

50,000 Units

100 µL 500 Units

Low DNA Taq HS 5 U/µL Heat-activated, thermostable DNA polymerase suited amplification of bacterial and fungal DNA.

MDX009

10 mL

50,000 Units

RNA Polymerase Glycerol-Free T7 RNA Polymerase (HC)

50 uLL 50,000 Units

A high concentration (1,000 U/µL) DNA-dependant RNA Polymerase with strict specificity to DNA template containing the T7 promoter sequence, it is an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications including preparation of mRNA on a commercial scale and point-of-care diagnostic applications (NASBA).

MDX201

500,000 Units

500 uL

High-Fidelity Polymerases High-Fidelity Pfu 3’ - 5’ proofreading exonuclease activity with an error rate of 3.0 x 10 -6 and generates blunt-ended amplicons up to 5 kb in length.

200 µL 500 Units

MDX003

10 mL

25,000 Units

Glycerol-Free High-Fidelity Pfu (HC) A high‑concentration (20 U/µL), glycerol‑free, high-fidelity thermostable DNA polymerase, a novel buffer system containing magnesium, dNTPs and excipients, to provide high yield and sensitivity, perfect for target enrichment, NGS library amplification, gene expression, cloning and site-directed mutagenesis.

25 µL

500 Units

MDX203

1 mL 20,000 Units

5 mL

200 Rxns

High-Specificity Pfu HS Mix High-fidelity, aptamer-based hot-start DNA polymerase tailored for low GC bias amplification.

MDX006

100 mL

4,000 Rxns

Isothermal Polymerases

1 mL 8,000 Units

Bst DNA Polymerase (8U/µL) Stable, heat resistant DNA polymerase optimized for LAMP and contains 5´ > 3´ DNA polymerase activity and strong strand displacement activity.

MDX012

160,000 Units

20 mL

80 µL 8,000 Units

High Conc. Glycerol-Free Bst (100U/µL) Lyophilization-compatible DNA polymerase for isothermal applications.

MDX018

800 µL 80,000 Units

Polymerase continued

Isothermal Polymerases

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

Glycerol-Free T7 RNA Polymerase (HC, 1,000 U/uL) A high concentration (1,000 U/µL) DNA-dependant RNA Polymerase with strict specificity to DNA template containing the T7 promoter Sequence. It is an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications including preparation of mRNA on a commercial scale and point-of-care diagnostic applications (NASBA).

50 uLL 50,000 Units

MDX201

500,000 Units

500 uL

50 μL 2,500 Units

Glycerol-Free RNase H (HC, 50 U/uL) An RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA when hybridized to DNA, and produces 5´ phosphate-terminaed oligoribonucleotides and single-stranded DNA.

MDX202

250 μL 12,500 Units

Reverse Transcriptase 55C MMLV-RT Thermostable reverse transcriptase with activity up to 60°C. Ideal for RNA with high secondary structure such as viral genomes. Formulation is compatible with lyophilization and air-drying applications.

200 µL 1,000 Units

MDX117

2 mL 10,000 Units

Lyo-compatible MMLV-RT High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays. RNase-Tolerant MMLV-RT Mixture of MMLV-RT and RNase inhibitor ideal for RT-qPCR assays.

8 µL 1,000 Rxns

MDX042

80 µL 10,000 Rxns

200 µL 1,000 Rxns 2 mL 10,000 Rxns

MDX043

200 µL 1,000 Rxns

MMLV-RT MMLV-RT suitable for generating first strand cDNA of up to 9 kb.

MDX044

2 mL 10,000 Rxns

Next-Generation Sequencing

NGS Clean and Select Beads Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries.

50 mL

MDX041

500 mL

Glycerol-free T4 DNA Ligase (HC) Glycerol-free, high concentration enzyme (50 U/μL) that catalyzes the formation of a phosphodiester bond between adjacent 5’ phosphate and 3’ hydroxyl groups in duplex DNA or RNA.

20 µL 1,000 Units

MDX200

200 µL 10,000 Units

NGS Library Quantification qPCR-based assay for the quantification of only adapter-ligated library molecules.

1 Kit

500 Rxns

MDX039

High-Fidelity Pfu (10x) High-fidelity PCR product containing a hot-start antibody, separate 10x Pfu Reaction Buffer and MgCl 2 , ideal for high yields in NGS library amplification. Glycerol-Free High-Fidelity Pfu (HC) A high‑concentration (20 U/µL), glycerol‑free, high-fidelity thermostable DNA polymerase, a novel buffer system containing magnesium, dNTPs and excipients, to provide high yield and sensitivity, perfect for target enrichment, NGS library amplification, gene expression, cloning and site-directed mutagenesis. High-Specificity Pfu HS Mix High-Specificity Pfu HS Mix highly suited for target enrichment, NGS library amplification, and cloning applications to provide superior amplification of GC- and AT-rich targets.

200 µL 500 Units

MDX003

10 mL 25,000 Units

25 µL

500 Units

MDX203

1 mL 20,000 Units

5 mL

200 Rxns

MDX006

100 mL 4,000 Rxns

www.MeridianBioscience.com/lifescience 9

Components

Buffers

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

Taq PCR Buffer, 5x Optimized for use with Taq DNA Polymerase (MDX001) and Taq HS DNA Polymerase (MDX008) . Bst Reaction Buffer, 10x Optimized for use with Bst DNA Polymerase (MDX012) and High Conc. Glycerol-Free Bst (MDX018) .

2 mL

500 Rxns

MDX002

5 mL

MDX076

10 mL

5 mL

Enzyme Dilution Buffer, 1x A glycerol containing 1x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX078

100 mL

5 mL

Enzyme Dilution Buffer (10x) (Glycerol free) A glycerol-free,10x dilution buffer, for the dilution of enzymes to reaction concentration.

MDX080

10 mL

Tissue Extract-PCR Buffers Lysis and neutralization buffer optimized for use with Taq HS DNA Polymerase (MDX008) to perform PCR direct from crude lysate.

1 Kit

1,000 Rxns

MDX004

2.5 mL 500 Rxns

Fast qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) .

MDX033

50 mL 10,000 Rxns

2.5 mL 500 Rxns

1-Step RT-qPCR Buffer, 4x Optimized for use with Taq HS DNA Polymerase (MDX008) and RNase-Tolerant MMLV-RT (MDX043) . Lyo-Ready ™ qPCR Buffer, 2.5x Optimized for use with Glycerol-Free Taq HS (MDX011) . In order to produce lyophilized, ambient-temperature stable qPCR reagents, dNTPs, MgCl 2 and glycerol-free Taq DNA polymerase are not included in the buffer.

MDX034

50 mL 10,000 Rxns

8 mL 1,000 Rxns

MDX022

100 mL 12,500 Rxns

2.5 mL 500 Rxns

Lyo-Ready ™ qPCR Buffer w/o Excipients, 4x Optimized for use with Glycerol-Free Taq HS (MDX011) .

MDX061

100 mL 20,000 Rxns

5 mL

Taq Dilution Buffer Taq Dilution Buffer provides the optimal conditions to store Meridian polymerases, conferring long-term stability at -20 °C (MDX008) . Inhibitor-Tolerant PCR Buffer, 5x Used with Taq HS DNA Polymerase (MDX008) for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Inhibitor-Tolerant Bst Buffer Optimized buffer for MDX012 and MDX018 that increases the amplification speed, sensitivity, and tolerance to salt and other inhibitors found in clinical samples and transport media. General Reagents Uracil DNA Glycosylase (UDG)* Enzyme that efficiently hydrolyzes uracil from ssDNA or dsDNA. Endonuclease, exonuclease, nickase and RNase-free. Proteinase K Solution RNase and DNase free, ideal for removing endogenous nucleases when purifying native DNA or RNA.

MDX007

100 mL

2 mL

500 Rxns

MDX075

100 mL 20,000 Rxns

2 mL

MDX019

100 mL

10 mL

10,000 Units

MDX054

25 mL

500 mg

MDX055

250 µL 10,000 Units

RNase Inhibitor Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C to control for contaminants in RT-PCR assays.

MDX056

100,000 Units

2.5 mL

# Uracil DNA Glycosylase (MDX054) is not available in the US

Components continued

General Reagents continued

PRODUCT

APPLICATION CAT NO. VOLUME REACTIONS

100 µL 4,000 Units

RNase Inhibitor (Glycerol Free) A highly efficient glycerol-free inhibitor of a broad spectrum of eukaryotic RNases that does not inhibition polymerase or reverse transcriptase activity.

MDX120

200,000 Units

500 µL

20 µL 1,000 Units

Taq HS Antibody A mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity at room temperature. For use in hot-start PCR.

MDX014

250 µL 12,500 Units

Ready-to-use sequencing grade dNTPs available individually, as sets, or pre-blended dNTP mixes. Ultra-pure (>99% by HPLC) free of PCR inhibitors, DNase and RNase.

dNTPs

dNTPs Lithium Salt

PRODUCT

CAT NO.

VOLUME

4 x 250 µL

dNTP Set, 100 mM lithium salt Provided as four separate solutions at 100 mM concentration (dATP, dCTP, dGTP & dTTP).

MDX050

4 x 50 mL

500 µL

50 mL

dNTP Mix, 100 mM lithium salt Supplied as a mixture (dATP, dCTP, dGTP & dTTP). Total concentration of mix is 100 mM (25 mM of each nucleotide).

MDX051

100 mL

1 L

dUTP Mix, 50 mM lithium salt Supplied as a mixture (10 mM dATP, dCTP, dGTP and 20 mM dUTP).

50 mL

MDX058

dUTP, 100 mM lithium salt

50 mL

MDX059

dATP, 100 mM lithium salt

50 mL

MDX046

dCTP, 100 mM lithium salt

50 mL

MDX047

dGTP, 100 mM lithium salt

50 mL

MDX048

dTTP, 100 mM lithium salt

50 mL

MDX049

* Not available in China

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dNTPs continued

dNTPs Sodium Salt

PRODUCT

CAT NO.

VOLUME

Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire Please Enquire

dNTP Mix, 10 mM sodium salt*

MDX067

dNTP Mix, 40 mM sodium salt*

MDX083

dNTP Mix, 100 mM sodium salt*

MDX084

dATP Mix, 100 mM sodium salt*

MDX063

dCTP Mix, 100 mM sodium salt*

MDX064

dGTP Mix, 100 mM sodium salt*

MDX065

dTTP Mix, 100 mM sodium salt*

MDX066

dUTP, 100mM, sodium salt*

MDX096-20

qPCR and RT-qPCR Extraction Controls

qPCR Extraction Controls

PRODUCT

CAT NO. VOLUME

REACTIONS

qPCR Extraction Control RED (Quasar ® 670) E. coli cells of a known concentration containing the Internal Control DNA sequence with no known homology to any organism, and supplied with Quasar ® 670 fluorophores for multiplex qPCR. qPCR Extraction Control ORANGE (Cal Fluor ® 560) E. coli cells of a known concentration containing the Internal Control DNA sequence with no known homology to any organism, and supplied with Cal Fluor ® 560 fluorophores for multiplex qPCR.

10 mL

2,000 Rxns

MDX026

10 mL

2,000 Rxns

MDX027

RT-qPCR Extraction Controls

PRODUCT

CAT NO. VOLUME

REACTIONS

1 mL

250 Rxns

VLP-RNA Extraction Control Red Virus-like particle (VLP) containing a defined number of copies of target RNA molecules ( 1x10 4 copies/µL ). supplied with Quasar ® 670 fluorophores for multiplex RT- qPCR. VLP-RNA Extraction Control Orange Virus-like particle (VLP) containing a defined number of copies of target RNA molecules ( 1x10 4 copies/µL ). supplied with Cal Fluor ® 560 fluorophores for multiplex RT- qPCR.

MDX068

20 mL

5,000 Rxns

1 mL

250 Rxns

MDX069

20 mL

5,000 Rxns

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