Polymerase continued
Isothermal Polymerases
PRODUCT
APPLICATION CAT NO. VOLUME REACTIONS
Glycerol-Free T7 RNA Polymerase (HC, 1,000 U/uL) A high concentration (1,000 U/µL) DNA-dependant RNA Polymerase with strict specificity to DNA template containing the T7 promoter Sequence. It is an ideal enzyme for in-vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications including preparation of mRNA on a commercial scale and point-of-care diagnostic applications (NASBA).
50 uLL 50,000 Units
MDX201
500,000 Units
500 uL
50 μL 2,500 Units
Glycerol-Free RNase H (HC, 50 U/uL) An RNA-specific endonuclease that hydrolyzes the phosphodiester bonds of RNA when hybridized to DNA, and produces 5´ phosphate-terminaed oligoribonucleotides and single-stranded DNA.
MDX202
250 μL 12,500 Units
Reverse Transcriptase 55C MMLV-RT Thermostable reverse transcriptase with activity up to 60°C. Ideal for RNA with high secondary structure such as viral genomes. Formulation is compatible with lyophilization and air-drying applications.
200 µL 1,000 Units
MDX117
2 mL 10,000 Units
Lyo-compatible MMLV-RT High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays. RNase-Tolerant MMLV-RT Mixture of MMLV-RT and RNase inhibitor ideal for RT-qPCR assays.
8 µL 1,000 Rxns
MDX042
80 µL 10,000 Rxns
200 µL 1,000 Rxns 2 mL 10,000 Rxns
MDX043
200 µL 1,000 Rxns
MMLV-RT MMLV-RT suitable for generating first strand cDNA of up to 9 kb.
MDX044
2 mL 10,000 Rxns
Next-Generation Sequencing
NGS Clean and Select Beads Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries.
50 mL
-
MDX041
500 mL
-
Glycerol-free T4 DNA Ligase (HC) Glycerol-free, high concentration enzyme (50 U/μL) that catalyzes the formation of a phosphodiester bond between adjacent 5’ phosphate and 3’ hydroxyl groups in duplex DNA or RNA.
20 µL 1,000 Units
MDX200
200 µL 10,000 Units
NGS Library Quantification qPCR-based assay for the quantification of only adapter-ligated library molecules.
1 Kit
500 Rxns
MDX039
High-Fidelity Pfu (10x) High-fidelity PCR product containing a hot-start antibody, separate 10x Pfu Reaction Buffer and MgCl 2 , ideal for high yields in NGS library amplification. Glycerol-Free High-Fidelity Pfu (HC) A high‑concentration (20 U/µL), glycerol‑free, high-fidelity thermostable DNA polymerase, a novel buffer system containing magnesium, dNTPs and excipients, to provide high yield and sensitivity, perfect for target enrichment, NGS library amplification, gene expression, cloning and site-directed mutagenesis. High-Specificity Pfu HS Mix High-Specificity Pfu HS Mix highly suited for target enrichment, NGS library amplification, and cloning applications to provide superior amplification of GC- and AT-rich targets.
200 µL 500 Units
MDX003
10 mL 25,000 Units
25 µL
500 Units
MDX203
1 mL 20,000 Units
5 mL
200 Rxns
MDX006
100 mL 4,000 Rxns
Point-of-Care | Environmental | Vet Health | Liquid Biopsy | Food Testing | Human Diagnostics |
www.MeridianBioscience.com/lifescience 9
Powered by FlippingBook