SARS-CoV-2 Recombinant Antigens

For COVID-19 Serology Testing. Serology testing is an important tool for monitoring the evolution of an outbreak and serology tests that detect neutralizing antibodies are particularly useful for evaluating vaccine-induced protective immunity.

SARS-CoV-2 Genome Structure

SARS-CoV-2 Recombinant Antigens For COVID-19 Serology Testing

Spike (S1 & S2)

Membrane (M)

Nucleocapsid (N)

ssRNA (+ sense, ~30kb in length)

Envelope (E)

Serology testing is an important tool for monitoring the evolution of an outbreak and serology tests that detect neutralizing antibodies are particularly useful for evaluating vaccine-induced protective immunity. As new SARS-CoV-2 variants emerge with mutations in the spike S protein, their potential to become “escape mutants” and negatively impact vaccine efficacy needs to be evaluated through neutralization testing. Meridian’s panel of SARS-CoV-2 mutant recombinant antigens represent the World Heath Organization’s major variants of concern and are suitable for use in neutralization studies and as controls in serology assays. They complement the existing range of high-performing SAR-CoV-2 wild-type antigens, several of which are in current EUA approved COVID-19 IgG/IgM serology tests.

SARS-CoV-2 Recombinant Antigens

Wild-type SARS-CoV-2

Mutant SARS-CoV-2 Spike, S1 RBD

Trimeric Spike, S1 Protein (His-Tag) Represents amino acids 14 - 683 of SARS-CoV-2 plus the synthetic trimeric domain Spike Protein, S1 Subunit (His-Tag) Represents amino acids 14 - 683 of the SARS-CoV-2 S1 subunit Spike Protein, N-terminal Domain (NTD) S1 Subunit (His-Tag) Represents amino acids 16 - 305 of the SARS-CoV-2 N-terminal domain Spike Protein, S1 Subunit RBD (His-Tag) Represents amino acids 330 - 521 of the SARS-CoV-2 S1 subunit, RBD domain

Omicron mutant (B.1.1.529.1) Represents Omicron variant with 15 mutations on the RBD

9544

9566

L452R and T478K mutant (His-Tag) Represents B.1.617.2 (Delta Variant), a.a. 319 - 541

9606

9556

N501Y mutant (His-Tag) Represents B.1.1.7 strain, (Alpha variant) a.a. 319 - 541 K417N, E484K and N501Y mutant (His-Tag) Represents B.1.351 strain, (Beta variant) a.a. 319 - 541 K417T, E484K and N501Y mutant (His-Tag) Represents P.1 strain, (Gamma variant) a.a. 319 - 541

9567

9557

9568

9558

9569

9560

Nucleocapsid Protein (His-Tag)

L452R mutant (His-Tag) Represents Epsilon variant, a.a. 319 - 541

9598

9552

Spike Protein, S1 Subunit RBD (His-Tag)

L452R and E484Q mutant (His-Tag) Represents B.1.167 strain (Kappa variant), a.a. 319-541

9599