ELISA 性能, COVID-19 单抗 9548 & 9547
检测步骤
加入 100μl样本稀释液， 37°C热浴 60 分钟 步骤1
步骤2
步骤3
步骤4
步骤5
清洗 (3 次)
加入100μl 偶联稀释液, 37°C热浴 30 分钟
清洗 (3 次)
加入100μl TMB, 室温10分钟
ELISA #1: 偶联标记: COVID-19 单抗 9548 – HRP 偶联 | 包被: COVID-19 单抗 9547 - 2μg/mL | 抗原: SARS-CoV-2核蛋白重组抗原
稀释
1/1,000
1/2,000
1/4,000
1/8,000
1/16,000
1/32,000
Blank
0.017 0.020 0.017 0.018 0.014 0.015 0.010 0.010 0.012 0.009 0.008 0.010 NaN NaN NaN NaN 3.711 3.749 2.680 2.715 1.758 1.812 1.046 1.194
10μg/mL 1μg/mL
3.881 NaN 3.482 3.586 3.033 3.077 2.249 2.259 1.397 1.414 0.869 0.920 500ng/mL 3.733 3.387 2.879 2.933 2.409 2.488 1.776 1.782 0.896 1.055 0.569 0.711 250ng/mL 2.970 2.692 2.290 2.334 1.865 1.956 1.333 1.330 0.814 0.806 0.447 0.481 100ng/mL 2.006 1.887 1.514 1.585 1.204 1.201 0.820 0.846 0.513 0.533 0.282 0.293 10ng/mL 0.254 0.241 0.143 0.192 0.138 0.135 0.080 0.087 0.050 0.054 0.029 0.028 1ng/mL 0.029 0.024 0.016 0.016 0.014 0.015 0.017 0.017 0.008 0.013 0.008 0.011
ELISA #2: 偶联标记: COVID-19 单抗 9548 – HRP 偶联 | 包被: COVID-19 单抗 9547 – 8μg/mL | 抗原: SARS-CoV-2核蛋白重组抗原
稀释
1/1,000
1/2,000
1/4,000
1/8,000
1/16,000
1/32,000
Blank
0.045 0.051 0.040 0.043 0.032 0.033 0.022 0.021 0.016 0.015 0.012 0.012 NaN NaN NaN NaN NaN NaN NaN NaN 2.886 2.883 1.717 1.814 NaN NaN NaN NaN NaN NaN 3.833 3.833 2.719 2.705 1.641 1.704 NaN NaN NaN NaN NaN NaN 3.502 3.439 2.280 2.347 1.386 1.429
10ug/mL 1ug/mL 500ng/mL 250ng/mL
NaN NaN NaN NaN 3.715 3.567 2.817 2.836 1.816 1.726 1.004 1.075 100ng/mL 3.571 3.433 2.915 2.957 2.373 2.281 1.662 1.616 1.016 1.008 0.517 0.561 10ng/mL 0.475 0.445 0.347 0.349 0.240 0.232 0.152 0.150 0.088 0.088 0.042 0.047 1ng/mL 0.055 0.099 0.045 0.063 0.044 0.043 0.029 0.028 0.015 0.019 0.011 0.013
活病毒检测 抗体对9547和9548可与活病毒反应。通过测流免疫检测，结果显示， 该抗体对可检测出约 2 x 10 3 TCID 50 /mL COVID-19 灭活病毒 (来自Zeptomatrix, USA)，或 2 x 10 1 TCID 50 /mL COVID-19 活病毒。
测流免疫检测结果
对照组
实验组
订购信息: Sara Zhou
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