Tropical & Vector-Borne Diseases

Tropical & Vector-Borne Diseases ANTIGENS & ANTIBODIES FOR COMMERCIAL IMMUNOASSAY DEVELOPMENT

Tropical and vector-borne diseases account for more than 17% of all infectious diseases, causing more than 1 million deaths annually. Recognizing infected patients early in their clinical course is critical to preventing outbreaks. Meridian offers a range of antigens and antibodies suitable for the development of sensitive and specific immunoassays that detect tropical and vector-borne diseases. TROPICAL & VECTOR-BORNE DISEASES • Alphavirus • Borrelia burgdorferi (Lyme) • Borrelia garinii • Chagas • Chikungunya • Dengue • Ebola • Japanese Encephalitis Virus • Lassa Virus • Leishmania • Leptospirosis • Malaria • Marburg Virus • Mayaro Virus • Monkeypox • Newcastle Disease • Nipah Virus • Tick-borne Encephalitis Virus • Tsutsugamushi • West Nile Virus • Yellow Fever Virus • Zika Virus Reagents Suitable for Rapid Assays

Pairs Table

CAPTURE DETECTION

CAPTURE DETECTION

CAPTURE DETECTION

C01914M C01913M C01914M C01652M C01652M C01649M C01652M C01650M C01652M C01651M C01838M C01961M BN1087 BN1088 BN1088 BN1087 C01964M C01965M BN1170 BN1171 BN1171 BN1170 BN1169 BN1171 BN1170 BN1171 BN1171 BN1170 BN1169 BN1171 BN1176 BN1175 BN1176 BN1176 C01976M C01978M C01976M C01979M C01976M C01980M C01977M C01978M C01977M C01979M C01977M C01980M BN1161 BN1161

Lassa NP BN1168 BN1167 Malaria (Pan HRP-2) C01817M C01816M Malaria ( P. falciparum pLDH) C01833M C01835M Malaria (Pan pLDH) C01834M C01835M

C01912M C01906M C01912M C01907M C01912M C01911M C01912M C01910M C01912M C01909M C01906M C01911M C01906M C01907M C01906M C01909M C01906M C01910M C01909M C01911M C01909M C01910M C01909M C01907M C01907M C01911M C01907M C01910M C01910M C01911M

Dengue Virus NS1

C01584M C01586M C01584M C01585M C01836M C01837M

Malaria ( P. falciparum HRP-2)

Yellow Fever NS1

Nipah Virus G protein

C01975M C01974M

BN1163 BN1166 BN1163 BN1164 BN1164 BN1166 BN1165 BN1166 C01867M C01868M C01887M C01888M C01888M C01888M C01887M C01889M C01888M C01890M C01889M C01888M BN1092 BN1094 BN1094 BN1092

Ebola NP

Tsutsugamushi

Ebola VP40

Ebola sGP

Zika Virus NS1

Antigen/Antibody Pairs

ANTIGEN ANTIBODY

Japanese Encephalitis Virus NS1 (JEV NS1)

R01710 C01930M C01914M C01931M C01838M C01932M

Malaria ( P. falciparum HRP-2)

R - Reversible | L - Lateral Flow

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ANTIBODIES

Ebola Virus (Continue) BN1170

Newcastle Disease Virus C01629M

Alphavirus C01643M ELISA, IFA Borrelia burgdorferi C65551M

(NP), ELISA, LF, pair (NP), ELISA, LF, pair (VP40) ELISA, LF, pair (VP40) ELISA, LF, pair (VP40) ELISA, LF, pair (sGP) ELISA, LF, pair (sGP) ELISA, LF, pair (NP), ELISA, LF, WB, pair (NP), ELISA, LF, WB, pair

(RNP) ELISA, IFA, IHC

BN1171 BN1172 BN1173 BN1174 BN1175 BN1176

C86012M C86014M

(HN Glycoprotein) ELISA, HIA (HN Glycoprotein) ELISA, HIA

ELISA, IFA, LF ELISA, IFA, LF

Nipah Virus G Protein C01974M ELISA, pair C01975M ELISA, pair Tsutsugamushi BN1163 ELISA, LF, pair BN1164 ELISA, LF, pair BN1165 ELISA, LF, pair BN1166 ELISA, LF, pair West Nile Virus, Envelope Protein C01538M ELISA, IFA C01541M ELISA, IFA C01542M ELISA, IFA Yellow Fever Virus NS1 BN1089 ELISA, LF BN1090 ELISA, LF BN1091 ELISA, LF C01906M ELISA, LF, pair C01907M ELISA, LF, pair C01908M ELISA, LF C01909M ELISA, LF, pair C01910M ELISA, LF, pair C01911M ELISA, LF, pair C01912M ELISA, LF, pair Zika Virus, Envelope Protein C01860M ELISA, LF C01861M ELISA, LF C01862M ELISA, LF C01863M ELISA, LF C01937M ELISA, IFA Zika Virus, NS1 Protein BN1092 ELISA, LF, pair BN1093 ELISA, LF BN1094 ELISA, LF, pair C01864M ELISA, LF C01865M ELISA, LF C01866M ELISA, LF C01867M ELISA, LF, pair C01868M ELISA, LF, pair C01869M ELISA, LF C01870M ELISA, LF C01887M ELISA, IFA, LF, pair C01888M ELISA, IFA, LF, pair C01889M ELISA, IFA, LF, pair C01890M ELISA, IFA, LF, pair

C65550M B65302R

ELISA, IFA, IHC(p), WB (Rabbit PAb)

Chikungunya Virus BN1079

C01964M C01965M C01966M C01967M C02027M C02026M

ELISA, LF ELISA, LF ELISA, LF ELISA, LF ELISA, LF ELISA, LF ELISA, LF ELISA, LF

(NP), ELISA, WB (NP), ELISA, WB (NP) ELISA, WB

BN1080 BN1081 BN1082 BN1083 BN1084 BN1085 BN1086

(GP) ELISA

C02025S (VP40, NP, GP) ELISA Japanese Encephalitis Virus NS1 (JEV NS1) BN1161 ELISA, LF, pair BN1162 ELISA, LF, C01976M ELISA, pair C01977M ELISA, pair C01978M ELISA, pair C01979M ELISA, pair C01980M ELISA, pair Lassa Nucleoprotein BN1167 ELISA, LF, pair BN1167 ELISA, LF, pair Leptospira biflexa B65401R ELISA, IFA (Rabbit PAb) Malaria (Pan) C66509M (pLDH) ELISA, LF C01835M (pan pLDH) ELISA, LF, pair C01834M (pan pLDH) ELISA, LF, pair Plasmodium falciparum (Malaria) C01584M (HRP-2) ELISA, LF, pair C01585M (HRP-2) ELISA, LF, pair C01586M (HRP-2) ELISA, LF, pair C01816M (HRP-2) ELISA, LF, pair C01817M (HRP-2) ELISA, LF, pair C01836M (HRP-2) ELISA, LF, pair C01837M (HRP-2) ELISA, LF, pair C01833M (pLDH) ELISA, LF, pair C86943M (MSP-1) ELISA, WB C01930M (HRP-2) ELISA, LF, pair Plasmodium vivax (Malaria) C86328M (CSP) ELISA, WB C86634M (MSP1) ELISA, WB C86636M (MSP1) ELISA, WB Marburg Virus C01424M ELISA, WB C01425M ELISA, WB C01426M ELISA, WB

C01640M C01641M C01642M

ELISA, IFA, LF ELISA, IFA, LF ELISA, IFA, LF

Chikungunya Virus, Envelope Protein C01891M ELISA, LF C01892M ELISA, LF C01893M ELISA, LF C01894M ELISA, LF C01895M ELISA, LF Dengue Virus C03045M

(Types 1, 2, 3, 4) ELISA, IFA, DB

Dengue Virus NS1 BN1087

ELISA, LF, pair ELISA, LF, pair ELISA, IFA, pair ELISA, IFA, pair ELISA, IFA, pair

BN1088

C01649M C01650M C01651M C01652M C01838M C01896M C01899M C01913M C01914M C01898M C01961M

ELISA, IFA, LF, pair

ELISA, LF ELISA, LF ELISA, IFA

ELISA, IFA, LF, pair ELISA, IFA, LF, pair

ELISA, IFA

ELISA, LF, pair

Ebola Virus BN1169

(NP), ELISA, LF, pair (VP40), ELISA, WB (VP40), ELISA, WB (VP40), ELISA, WB (VP40), ELISA, WB (VP40), ELISA, WB (VP40), ELISA, WB (VP40) ELISA, WB (VP40) ELISA, WB

C01968M C01969M C01970M C01971M C01972M C01973M C02028M C02029M

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ANTIGENS

Borrelia afzelii R01576, E.coli

Dengue Virus Type 2 R02220, Native ELISA, LF R01657, Drosophila

Plasmodium falciparum (Malaria) BN1128, E.coli (HRP2), ELISA,LF R01478, E.coli (HRP-2) ELISA, LF R01596, E.coli (HRP-2) ELISA, LF, WB R01710, E.coli (HRP-2) ELISA, LF, pair BN1126, HEK (pLDH), ELISA, LF R01597, E.coli (pLDH) LF R01595, E.coli (pAldolase) LF R01603, E.coli (MSP1) ELISA, LF R01604, E.coli (MSP1) ELISA, LF Plasmodium vivax (Malaria) BN1127, HEK (pLDH) ELISA, LF R01439, E.coli (MSP1) ELISA, LF R01440, E.coli (CSP) ELISA, LF R01598, E.coli (pLDH) ELISA, LF, WB R01601, E.coli (MSP1) ELISA, LF R01602, E.coli (MSP1) ELISA, LF Tick-borne Encephalitis Virus R18082, E.coli (Glycoprotein E) ELISA, WB Tsutsugamushi BN1177, E.coli (GST-tag)ELISA, LF

(Osp-C) ELISA, WB (VlsE) ELISA, WB (p41), ELISA, WB

R01609, E.coli R01707, E.coli

(NS1) ELISA, LF, WB (Envelope) ELISA, LF (prM Envelope) ELISA

R01660, Drosophila

R01767, Insect

Borrelia burgdorferi R8A131, E.coli

Dengue Virus Type 3 R01688, Native ELISA, LF R01661, Drosophila

(Osp-A) ELISA, LF (Osp-C) ELISA, LF (VlsE) ELISA, WB

R8A123, E.coli R01523, E.coli R01526, E.coli

(Envelope) ELISA, LF

(p14 Flagellin), ELISA, LF, WB

R01658, Drosophila

(NS1) ELISA, LF

Dengue Virus Type 4 BN1131 E.coli

Borrelia garinii R01521, E.coli

(VP40) ELISA, LF (VP40) ELISA, LF

(p14 Flagellin) ELISA, LF, WB, DB

BN1132 E.coli R01689, Native

ELISA, LF

R01610, E.coli (VlsE) ELISA, LF, CLIA, WB Chagas (Trypanosoma cruzi) R01589, E.coli (FRA) ELISA, LF, WB R01587, E.coli (1F8) ELISA, LF, WB

R01662, Drosophila

(Envelope) ELISA, LF (NS1) ELISA, LF, WB

R01591, E.coli

R01663, Drosophila

(NS1) ELISA, LF

Lassa BN1178, Baculovirus E LISA, LF Leishmania R01513, E.coli

R01436, E.coli R01364, E.coli

(1F8) ELISA, LF

(PEP-2, TcD, TcE and SAPA) ELISA, LF (Chimeric) ELISA, LF (Chimeric) ELISA, LF (Chimeric) ELISA, LF

( L. donovani KMP-11) ELISA, WB

R01684, E.coli R01685, E.coli R02001, E.coli

Monkeypox BN1179, HEK

West Nile Virus R8A104, E.coli

(A29L) ELISA,LF (A29L) ELISA,LF

ELISA, LF, WB

Chikungunya Virus R01653, Insect

BN1180, HEK

R8A560, E.coli

(Pre-M) ELISA, LF, WB

(W.T. gp E1) ELISA, LF, WB

R01654, Insect

(mutant gp E1) ELISA, LF, WB

Zika Virus BN1129, HEK R01635, Insect R01636, Insect

(NS1) ELISA, LF

R01768, Insect

(E1, E2, E3) ELISA

(Envelope) ELISA, CLIA, LF

(NS1) ELISA, CLIA, LF

Dengue Virus Type 1 BN1130, HEK ELSA, LF R01687, Native ELISA, LF R01656, Drosophila

(NS1) ELISA, LF

R01659, Drosophila

(Envelope) ELISA, LF

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TROUBLESHOOTING & USAGE TIPS

Envelope (E) Protein: The dominant protein present on a surface of the flavivirus virion and is a major target for neutralizing antibodies. It contains highly conserved regions therefore antibodies directed to the envelope protein tend to be cross-reactive. NS1 Protein: A glycosylated, membrane-associated, secreted glycoprotein with replication and immune evasion functions. NS1 antigens can be detected very early in infection (as early as 1 day post infection). In addition NS1 is serotype-specific, and for viruses such as Dengue where more than one serotype circulates, NS1 antigens enable virus serotyping by ELISA. The Flavivirus genera (e.g. Dengue, Zika, Japanese Encephalitis Virus, West Nile, etc) share epitopes which induce the development of cross-reactive antibodies. This leads to great difficulty in differentially diagnosing flaviviral infections, especially where flaviviruses co-circulate such as in tropical climates. Methods to increase IgG and IgM sensitivity 1. IgM-Capture Assays: Use anti-human IgM Fab fragment antibody as the capture (as opposed to a full-length anti-human IgM antibody). More Fab fragment antibodies are able to bind to the surface area of the solid substrate increasing the number of binding sites available for total IgM antibody that can be captured. 2. Lateral Flow Assays: Employ the bridging method in which colloidal gold-labelled disease specific antibody (e.g. MAb or PAb to Dengue NS1) is pre-mixed with recombinant antigen (e.g. Dengue NS1) and biotinylated anti-human IgM. Conjugating colloidal gold directly to the antigen can inhibit its ability to bind to captured IgM. Furthermore, using a gold-conjugated PAb, which has a broad reactivity, can further increase assay sensitivity. A PAb can bind to different antigen epitopes therefore enabling more than one PAb to bind to the same antigen simultaneously to generate a stronger signal.

Reducing cross-reactivity between flaviviruses in EIA assays To improve assay specificity, it is necessary to remove any cross-reacting antibodies that could bind to the antigen and cause a false result. Defined epitope blocking ELISAs have successfully been used to increase the specificity and for differentiating flaviviral infections through targeting epitopes on NS1 or E protein. By including low concentrations of unconjugated antigens representing the potential cross-reactive species, it is possible to block their binding to the target antigen.

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