DIAGNOSIS Diagnosis is usually made by detection of Toxo-specific IgG and IgM antibodies. A test that only measures IgG is used to determine if a person has previously been infected. When it is necessary to try to estimate the time of infection, such as in pregnancy, an IgM test is also used along with other tests such as an IgG avidity test. Diagnosis can also be made by direct observation of the parasite in stained tissue sections, cerebrospinal fluid (CSF), or other biopsy material. REAGENTS FOR SEROLOGY TESTING
8158
T. gondii Native Antigen • Purified whole tachyzoite preparation, RH strain • Propagated in Vero cells • Protein concentration: ~0.5 mg/mL by BCA Assay • Buffer: PBS, pH 7.4 containing 1% Triton x-100 T. gondii Native Antigen, Concentrated • Purified whole tachyzoite preparation, RH strain • Propagated in Vero cells • High protein concentration (~1mg/ml) • Buffer: PBS, pH 7.4 containing 1% Triton x-100
IgG & IgM Detection for ELISA and WB Assays
8200
8159
T. gondii Native Antigen, IgM • Purified membrane fraction (highly specific for Toxoplasma p30), RH strain • Protein concentration ~ 0.5 mg/mL (BCA Assay) • Buffer: Phosphate Buffered Saline, pH 7.4 containing 2% N-octyl- -D-Glucopyranoside (OGP). T. gondii Chimeric Recombinant Antigen • Contains immunogenic regions of the Toxoplamsa protein • Produced in P. pastoris and contains a His tag at the N-terminus • Buffer: 20 mM phosphate pH 7, 0.1 M KCL
IgM Detection for ELISA and WB Assays
R01794
IgG & IgM Detection for ELISA, CLIA and Lateral Flow
R01573
T. gondii p30 (SAG1) Recombinant Antigen • Recombinant protein (E. coli) , ≥ 95% pure (SDS-PAGE)
• Contains a His-tag at the N-terminus • Calculated molecular weight 36.6kDa • Protein concentration: ~0.8mg/ml • Buffer: 20 mM Phosphate Buffer, pH 8.0, 1 M Sodium Chloride, 0.1% Polyoxyethylene (10) Tridecyl Ether
IgG Detection for EIA Assays
ToRCH & Childhood Diseases- Reagents for Assay Development 10
Powered by FlippingBook