ToRCH & Childhood Diseases

REAGENTS FOR SEROLOGY TESTING

7420

EBV Native Antigen • Prepared from a detergent extraction of P 3 HR-1 or P 3 H 3 cells that have been induced by TPA and sodium butyrate • Partially purified >10% viral protein • Buffer: Carbonate, pH 9.5±0.2 EBV EBNA-1 Recombinant Antigen (full length) • Represents full length EBNA-1 and contains a His-tag at the N-terminus • Produced in E. coli • Buffer: 6M Urea, 20mM Phosphate, pH 8.0, 1M Sodium Chloride, 0.1% Polyoxyethylene(10)Tridecylether EBV p138 (EA-D) Recombinant Antigen • Represents the complete ORF of the BALF2 gene (Early Antigen D) • Contains a His-tag at the N-terminus • Produced in E. coli • 6M Urea, 20mM Phosphate, pH 8.0, 1M Sodium Chloride, 0.1% Polyoxyethylene(10)Tridecylether EBV VCA (gp125) Antigen • Purified antigen concentrate from P 3 H 3 cells • Concentration: ~ 30 μg/mL (Non-Interfering Protein Assay) • Buffer: 20 mM Tris-HCl, pH 7.4 containing 3M Magnesium Chloride

IgG Detection for ELISA and WB Assays

R01522

8202

IgM Detection for ELISA, WB, Lateral Flow and CLIA Assays

R01525

C66405M

MAb to EBV (gp125) • Reactive against EBV VCA gp125

C65023M

MAb to EBV (VCA) • Recognizes EBV, specific for VCA MAb to EBV (EBNA-1) • Specific for envelope glycoprotein complex 220/350

IFA Detection

C65221M

• EBV glycoprotein gp220/350 is the major glycoprotein associated with the EBV envelope (the 220 kd protein is the result of RNA splicing)

EBV VCA p23 Recombinant Antigen • Represents the ORF of the BLRF2 gene (a.a. 2-162) coding for the EBV tegument protein BLRF2 (23kDa) • Produced in E. coli (> 95% pure SDS-PAGE), lyophilized • Lyophilized from 20 mM Phosphate Buffer, pH 8, 1 M Sodium Chloride EBV VCA p18 Recombinant Antigen • Represents the truncated form of the viral capsid protein VP 26 (EBV gene BFRF3)

R01570

IgM Detection for ELISA and WB Assays

R01571

• Produced in E. coli (≥ 98% pure SDS-PAGE) • Buffer: 20 mM PBS @ pH 7 and 1 M NaCl