Mumps Mumps is an enveloped single-strand RNA virus of the Rubulavirus genus that causes painful swelling of the salivary glands. It is highly contagious and predominantly affects children.
The mumps virus resides in the mucus of the nose and throat of an infected person and it is mainly spread through coughing and sneezing. Symptoms may not appear for 12-25 days after transmission, however an infected person is contagious from 3 days prior to the onset of symptoms to 9 days after. In general, only supportive care is needed to resolve a mumps infection but occasionally it causes serious complications including meningitis, encephalitis, deafness and orchitis (inflammation of the testicles in males). Before the routine vaccination program was introduced in the United States (in 1967) and other countries, mumps was a common illness in infants, children and young adults. However, due to vaccination the disease is becoming rare. DIAGNOSIS
COUNTRIES USING MUMPS VACCINE IN NATIONAL IMMUNIZATION SCHEDULE, 2012
No (74 countries or 38%) Yes (120 countries or 62%) Not available Not applicable
Standard assays that detect mumps include viral detection methods, RT-PCR and serologic assays in both EIA and IFA formats. Specifically, assays that detect both IgM and IgG antibodies work well for diagnosing mumps infection in immunologically naïve individuals. However, the IgM response and viral shedding that occurs in persons who have been previously vaccinated or naturally infected are moderate in duration and intensity, making detection difficult. Recent research has shown that the enzyme-linked immunospot (ELISPOT) assay could be used as a more reliable diagnostic. This immunoassay method is based on the sandwich ELISA technique and is highly sensitive and the enables detection of activated mumps-specific, antibody-secreting B cells in whole blood.
REAGENTS FOR SEROLOGY TESTING
8099
Mumps Native Antigen • Prepared from a glycine extraction of infected (Enders Strain) LLC-MK2 cells • >10% viral protein, partially purified to reduce host cell components • Protein concentration: 0.2-1.0mg/mL by OD 260/280nm • Buffer: 0.1M Glycine, pH 9.3–9.7 Mumps Grade III Native Antigen • Prepared from infected (Enders Strain) LLC-MK2 cells • Purified by ion exchange chromatography to remove host cell protein • Buffer: 0.1 Na 2 CO 3 , 0.1M NaCl, pH 7.9-8.3
IgG Detection for EIA Assays
EV9529
IgM Detection for EIA Assays
C01719M C01720M C01721M
MAb to Mumps Virus Nucleoprotein • Reacts with Mumps Virus Nucleoprotein
ELISA & IFA Detection Assays
24
ToRCH & Childhood Diseases- Reagents for Assay Development
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