ToRCH & Childhood Diseases

DIAGNOSIS Laboratory diagnosis of enterovirus is important given that they can cause serious infections and surveillance is required to manage recurring outbreaks. Diagnostic methods include virus isolation, nucleic acid testing (NAT), and serological tests such as ELISA, complement fixation (CF), and neutralization assays. In particular, IgM EIAs have proven very useful due to their high specificity with the benefits of a lower cost and a reduced need for experienced personnel and dedicated laboratories. The application of IgM EIA is especially important in cases of meningitis when cerebral spinal fluid samples are not available and for the diagnosis of other enterovirus diseases with other clinical symptoms such as fever, enteritis, and HFMD. Also, the rapid serotype identification of enterovirus is important in differentiating non-poliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination, especially in age groups in which oral poliovirus vaccines are usually administered. REAGENTS FOR SEROLOGY TESTING

C01670M

MAb to Enterovirus Pan-reactive VP3 • Reacts with VP3 of many enterovirus types MAb to Enterovirus 70 • Reacts to most enterovirus type 70 • Non-reactive to many other Enteroviruses

Antigen Detection ELISA and IFA Assays

C01700M

• Specifically reactive with: rhinovirus Type 1a, 2, 14, 16, 17, 27, 42, 70, 80, coxsackie A7, A9, A16, A2, coxsackie B1, B2, B3, B4, B5, B6, echovirus Types 4, 6, 9, 11, 30, 34, EV71 and poliovirus

C30510M

MAb to Poliovirus 3 • Developed using the Sabin vaccine strain of poliovirus 3 MAb to Poliovirus 1 • Developed using the Sabin vaccine strain of poliovirus 1 Enterovirus A Coxsackievirus A16 Recombinan Antigen • Produced in E. coli (> 85% Purity) • Buffer: Tris Buffer, pH 8.0, 0.09% Sodium Azide Enterovirus A EV71 Recombinant Antigen • Produced in E. coli (> 85% Purity) • Buffer: Tris Buffer, pH 8.0, 0.09% Sodium Azide Enterovirus D EV70 Recombinant Antigen • Produced in E. coli (> 85% Purity) • Buffer: Tris Buffer, pH 8.0, 0.09% Sodium Azide

Antigen Detection ELISA and IFA Assays

C30027M

R01763

Control in Lateral Flow Assays and Immunogen

R01764

R01765