Sensitive detection in multiplex assays using sputum samples (up to 60% sputum) or Universal Transport Media (up to 50% UTM) swab containing sputum
Lyo-Ready ™ Direct DNA qPCR Saliva
Mycoplasma pneumoniae
ADV
CMV
0% Artificial sputum | 10% Artificial sputum | 20% Artificial sputum | 60% Artificial sputum
Mycoplasma pneumoniae genomic DNA, adenovirus (ADV) and cytomegalovirus (CMV) were amplified in a triplex qPCR assay in the presence of 0% ( Black ), 10% ( red ), 20% ( blue ) and 60% ( green ) artificial sputum. The results illustrate that the performance of the Lyo-Ready ™ Direct DNA qPCR Saliva is not affected by increasing concentrations of sputum.
Lyo-Ready ™ Direct RNA/DNA qPCR Saliva
Influenza A
MERS-CoV
RSV
MDX133 | TaqPath™ 1-Step | UltraPlex® 1-Step ToughMix
Three respiratory pathogens, Influenza A, Middle East Respiratory syndrome coronavirus (MERS-CoV) and Respiratory Syncytial Virus (RSV) were amplified in a triplex qPCR assay in the presence of 50% Universal Transport Media (UTM) swab containing sputum. The results illustrate that a higher multiplexing capacity and reproducibility was achieved with lyophilized Lyo-Ready ™ Direct RNA/DNA qPCR Saliva ( red ) compared to TaqPath ™ 1-Step Multiplex Master Mix ( black ) or UltraPlex ® 1-Step ToughMix ( grey )
Efficient amplification using the mix in a wet or dried down format
Lyo-Ready ™ Direct DNA qPCR Saliva
Lyo-Ready ™ Direct DNA qPCR Saliva was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100 and 10 copies respectively), in presence of 20% artificial sputum in both liquid ( blue ) and lyophilized ( red ) formats. The results illustrate that the lyophilized mix retains the ability to efficiently amplified to the same level as the liquid mix with the same level of sensitivity and reproducibility.
10,000 copies 100 copies 1,000 copies 10 copies
Lyophilized | Liquid
Powered by FlippingBook